Implementation of HPLC, UPLC-MS, and GC-MS techniques for characterizing non-model bacteria engineered for bio-based chemical production. Development of sample prep workflows and analytical methods implemented for waste stream/feedstock characterization, fermentation media optimization, intracellular carbon flux of engineered pathways, and product titer measurements.

Development of analytical methods, labeling techniques, and sample prep workflows to analyze human milk oligosaccharide production in engineered microbes.

Execution and optimization of LC-MS based structural analysis of therapeutic antibodies in a cGMP environment to support commercial lot testing and clinical trials.

Established, optimized, and executed HPLC and LC-MS methods to support structural characterization of screening proteins used to identify autologous T-Cell reactivity for personalized cell therapies. Optimized, validated, and executed mammalian protein expression, protein purification, and protein characterization methods at high-throughput and classical scales in support of T-cell discovery and validation of activity.

Development, validation, and optimization of analytical techniques in support of high-throughput assay development, enzyme discovery, protein engineering, and mechanistic enzymology efforts relevant to renewable biofuels manufacturing.

Investigation of pre-steady state and steady-state kinetic mechanisms in Complex II and Ferric Reductase. Anaerobic techniques, stopped flow analyses, and kinetic modeling were used to elucidate electron transfer mechanisms.

Investigation of protein motions in mutant alcohol dehydrogenases using time-resolved fluorescence spectroscopy. Enzyme mechanisms and biophysical properties of mutant proteins were characterized using a variety of chromatographic and spectroscopic methods.

Synthesis, purification, and structural determination of three benzyl-alkylated derivatives of iodoacetamide-capped tags suitable for peptide conjugation. Peptides were tested for increased sensitivity by LC-MS.

Characterized electrochemical properties of carboxylate-functionalized multi-walled carbon nanotube membranes as a function of pH.