Laboratory Duties- Operated batch and fed batch fermentations in shake flasks (125mL to 2.8L), benchtop scale (250mL-6L), pilot scale (15L-150L) and automated systems (ambr250)- Maintained seed train for various algal strains- Optimized various flask processes for titer and specific growth rate- Processed algal suspension through a spray drier and a freeze drier- Processed offline fermenter samples with use of a plate reader, cell counter, incubator and microscope- Ran offline media analytics using the Roche Cedex BioHT automated liquid handler- Conducted 96 well plate assays for additional offline media analytics and CMO quality control- Calibrated pH and DO probes, prepared addition bottles, and primed lines prior to run start- Maintained persistent batch records- Wrote detailed run reports and contributed to small team experimental planning- Cleaned, maintained and set up all available control systems for high throughput fermentation- Constant and consistent use of aseptic techniqueLab Manager Duties- In charge of consumable and equipment procurement in a time of pandemic plastic shortages- Maintained consumable supply and kept supply room organized and tidy- Coordinated purchase orders from various vendors

- Conducted supporting tasks in contribution to asset training, health, and care- Assisted in bioacoustic research sessions- Participation in roving patrols concerning nighttime asset care and security

- Cultured wheat and rice tissue for calli formation and later transformation- Dissected seeds and isolated embryos - Purified endosperm for protein analysis- Gene transformation by agrobacterium, heat shock, and electroporation in an aseptic environment- Isolated DNA from bacterial cells and plant tissue- Conducted restriction digests using a number of nucleases and plasmids- PCR and gel electrophoresis - Media making- Maintained a clean lab space to ensure efficient workflow

- Extracted DNA and RNA from tomato fruit to genotype a segregating population- Planted seedlings- Sampled leaf tissue- Assessed fruit weight- PCR and gel electrophoresis

- Assisted the excision of hearts from mice and rabbits for cardio myocyte study- Maintained a clean lab environment and ensured supplies were well stocked and accessible- Autoclaved glassware

- Conducted a study comparing P450 enzyme activity and hemolytic vitality between two populations of Mediterranean mussels- Carried out propidium iodide stains, EROD assays, - Designed an air flow system for mussel containment- Dissected mussel gill tissue- Assessed creosote based polycyclic aromatic hydrocarbon contamination

- Fed a variety of marine organisms including a leopard shark, tropical fish, and touch tank animals- Prepared food for animals- Cleaned tanks of algae, changed water, assessed water quality- Troubleshot pump and heating systems- Taught elementary school students about the exhibits