Manufactured CRISPR Cas9 knock-in constructs in both plasmid and AAV format from cloning to validation on cell linesAssisted with developing new standard operating protocols (SOP) for manufacturing and Quality Control (QC)Designed gRNAs through Benchling’s molecular biology platform via specific genes of interest Cloned plasmid constructs via Phusion, In-Fusion, transformation, backbone and vector creation, creation of stable cells for transformations, and validated constructs via colony PCR and sequencingPurified Adeno-associated virus (AAV) using ultracentrifugation method with iodixanol gradientCell culture: Aseptic technique maintaining multiple cell lines (HEK-293 WT/Cas9, HUVEC, U2OS), thawing/recovery of cryopreserved cells, cell passaging, cell counts, and transfection of CRISPR constructsRan experiments using immunohistochemistry (IHC), microscopy, and flow cytometric analysis for reagent validationWorked in a biotech accelerator environment with shared lab space and equipment requiring reservations

Assisted the lab's investigation of rodent heart and spinal cord regeneration as a hands-on research technician Experience in bacteria colonization, plating of bacteria, DNA extraction from bacteria, cloning, sequencing of DNA, transfection, and creating glycerol stocks for future useProficiency in running Western Blots from primary tissues with chemiluminescent and fluorescent imaging Conducted RNA extractions, cDNA production, qPCR assays, DNA purification, and PCR (tissue biopsy, digestion, gel electrophoresis and imaging)Involvement with mouse echocardiographs involving echo mouse prep, imaging, data collection and extractionSectioning of mouse and zebrafish organs and slide stainingOrganized breeding and maintenance of over 50 genetic mouse models. Provided post-surgical care for spinal cord transected mice. Mouse drug dosing via I.P, I.V. (tail vein) and SC. Maintenance of mouse database including mouse transfers, colony numbers, and management. Created SOPs and trained lab members

Managed animal orders including the production of over 100 transgenic and immunocompromised (nude) mice per month for principal investigators Created unique genetic compositions, interpreted genotyping results, and maintained detailed pedigree recordsExperience with animal restraint and handling, injections, surgical techniques, necropsy, animal identification, and euthanasia according to AVMA guidelinesProvided excellent customer service and detailed inventories for PI’s, also facilitated animal transfers and importsTrained new employees in colony management as well as educated and guided researchers in proper breeding and colony management, including advising appropriate mouse numbers and genetics for experimental designs

· Experience scheduling, communication between different factions within a production and cast members, and maintaining budgeting and expense reports· Managing and organization of film production and set building as well as running front of house