Tj Cradick, Ph.D. Email & Phone Number

Cambridge, MA, US

Shanghai, CN

https://GeneEditingFrontiers.com Providing fractional CSO functions, plus a consultant & advisor on all aspects of Gene Editing, Gene & Cell Therapy, including CRISPR, TALENS, Zinc Finger Nucleases, Base Editing and Gene Writing, Bioinformatics & Computational Biology (including CRISPR off-target assays) and FDA filings.Strategy, experimental design, due.

Independent Scientific Advisory Board member.

Charleston, South Carolina, US

Independent Scientific Advisory Board member.

San Francisco, CA, US

Excision is developing CRISPR-based therapies to cure viral infectious diseases and improve the lives of chronically ill patients. Excision EBT-101 clinical trial for HIV was awarded Fast Track designation and continues to enroll. Pipeline projects include HIV, HBV, and HSV. https://www.Excision.Bio/

Consultant & Advisor on Gene Editing, Gene & Cell Therapy.CRISPR, TALENS, Zinc Finger Nucleases. Bioinformatics & Computational Biology. Strategy, experimental design, due diligence, IP.

Boston, MA, US

Head of Genome Editing and 2nd U.S. employee. CRISPR Therapeutics is translating CRISPR-Cas9, a breakthrough genome-editing technology, into transformative medicines to cure human diseases. Mutations in genes that are causing diseases are specifically targeted by CRISPR-Cas9 and repaired using the cellular repair machinery. Research in the Genome Editing.

Atlanta, Georgia, US

♦ Developed CRISPR gene editing technologies, including methods to characterized nuclease specificity to drive further improvements. This research resulted in 21 publications and book chapters (so far), plus several patents (see below).♦ Developed and patented exhaustive CRISPR on- and off-target search method, including mismatches and /or differences in.

Molecular and cell biology consulting (genetics, recombination, cloning and expression systems, assay development) for local and international clients.

♦ Started as consultant, then given equity.♦ Advised on molecular and cellular biology methods and technology, protein engineering, literature, and filings for a biotech startup company focused on gene and gene therapy delivery.♦ Wrote declaration and contributed to the patent filings.

Iowa City, Iowa, US

Developed 14 sets of validated Zinc Finger Nucleases to create disease models in Cell Lines, Rat, Mice and Zebrafish.Set up cloning for Viral Vectors for our Zinc Finger Nucleases.Developed Bioinformatics and web interface that designs Zinc Finger Nucleases that target user-specified gene segments.ZFN-Site searches genomes for zinc finger nuclease target.

Iowa City, Iowa, US

Developed Zinc Finger Nucleases (ZFNs) that specifically cleave Hepatitis B Viral (HBV) DNA. Developed ZFN-site bioinformatic tool to help design zinc finger proteins and scan genomes for possible on-target and off-target sites. Developed assays to measure cleavage and mis-repair of ZFN sites and off-target sites:One of two groups to develop and first.

Brisbane, California, US

• Part of Zinc Finger and Zinc Finger Nuclease design (protein engineering) and selection group charged with producing DNA-binding domains with high activity and specificity.
• Developed new in vitro display selection technologies. Selected and tested ZFPs using Bacterial Two-Hybrid, SELEX and other systems.
• Chose genomic targets using Bioinformatics, Southern Analysis and DNase Hypersensitivity Mapping.
• Constructed artificial transcription factors with ZFPs fused to Activation and/or Repression Domains, transfected cells, and prepared RNA for analysis. Improved standard protocols and help troubleshoot these processes.
• Designed and optimized Taqman Primers and Probes and quantitated resulting changes in mRNA expression using Taqman and Affymetrix. Further analyzed the role our molecules played in gene regulation using Bioinformatic.
• Biochemical studies of ZFPs, including Gel-Shift and Off-Rate experiments and Alanine Screens.AC Jamieson, Bo Guan, TJ Cradick, H Xiao, PD Gregory and PM Carroll. Controlling gene expression in transgenic Drosophila.

US

Designed and developed a novel series of phage display libraries for use in high-throughput cellular assays.

San Francisco, California, US

• Thesis: Selection of Proteolytic Antibodies Against HIV gp120 (Advisor, Dr. Matthias Wabl).
• Developed Novel Phage-Based Selection for proteolytic antibodies. Tested via HPLC.
• Devised method to screen very large Antibody Libraries:
• Cloned Very Large Phage Display Antibody Libraries, both naïve and from mice I immunized with transition-state analogs.
• Assisted with troubleshooting email-based querying of Kabat Immunologic gene database for V-region primer design and other projects.

• Immunology consultant for law office handling cases of individuals with suspected autoimmune responses to breast implants.
• Advised on literature, methods, current research, and possible further experimentation.

Waltham, MA, US

• Brought in-house, developed and optimized a phage display library system and wrote SOPs for mapping the binding domains of anti-HIV antibodies and cell surface proteins. Screened phage displayed peptide libraries for.
• Engineered and expressed fusion proteins, and developed a phage expression system for structure/function analysis of protein domains for small molecule drug discovery.
• Prepared HIV clones using Long PCR for syncitia formation and neutralization assays.CL Jellis, TJ Cradick, P Rennert, P Salinas, J Boyd, T Amirault and GS Gray. Defining critical residues in the epitope for a.

Boston, Massachusetts, US

• May 1988 - Oct. 1989
• Effects of SV40 large T and small t antigens on transcriptional control.
• Operation of, and training others for use with the molecular core facility.
• Rebuilt and operated the oligo. synthesizer and assisted others with PCR primer design.

Cambridge, MA, US

Cambridge, MA, US

Tested polymers for drug delivery in Robert Langer's Laboratory.

Ph.D., Molecular And Cellular Biology

Ma, Immunology

Bs, Life Sciences

Semester At Queen Mary And Westfield College, Part Of The University Of London.

Education record