Tj Cradick, Ph.D. Email and Phone Number
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TJ Cradick PhD is the Chief Technology Officer (CTO) at HuidaGene Therapeutics, a global, clinical-stage biotech company that has developed a number of propriatary gene editing toolsIn additions the other positions, TJ consults on CRISPR, Gene Editing, and Epigenetic, building and advising new Gene Editing companies. See consulting info, my papers and presentations athttps://GeneEditingFrontiers.comPreviously, TJ Cradick was the Chief Scientific Officer (CSO) at Excision BioTherapeutics, a clinical-stage biotech company that is developing CRISPR-based therapies to cure viral infectious diseases and improve the lives of chronically ill patients. TJ led Excision's scientific vision and strategy, oversaw the research and development of its pipeline projects, and managed collaborations with academic and industry partners. TJ is the former Head of Genome Editing at CRISPR Therapeutics. As the 2nd US CRISPR Tx employee, he had many responsibilities through the company’s progression to a clinical stage company with >300 employees. TJ was faculty and Director of the Protein Engineering Facility at the Georgia Institute of Technology, where the research included bioinformatics and assays for CRISPR / Cas9 on- & off-target events.TJ is a recognized leader and innovator in the field of CRISPR/Cas9 technology, having published several influential papers on its applications, mechanisms, and optimization. He is also a Scientific Advisory Board Member at Eucalyptus Bio (CRISP-HR Therapeutics, Inc.) and at Catalytic Data Science. TJ is passionate about advancing the science and practice of CRISPR/Cas9 to address unmet medical needs and transform the lives of patients.
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Cso And PrincipalGene Editing Frontiers LlcCambridge, Ma, Us -
Chief Technology OfficerHuidagene Therapeutics Oct 2024 - PresentShanghai, Cn -
Cso / PrincipalGene Editing Frontiers Llc Apr 2024 - Presenthttps://GeneEditingFrontiers.com Providing fractional CSO functions, plus a consultant & advisor on all aspects of Gene Editing, Gene & Cell Therapy, including CRISPR, TALENS, Zinc Finger Nucleases, Base Editing and Gene Writing, Bioinformatics & Computational Biology (including CRISPR off-target assays) and FDA filings.Strategy, experimental design, due diligence, IP (and of course, CRISPR IP).Currently working on FDA filings, addressing clinical holds, advising on strategy and NewCo creation. -
Scientific Advisory Board MemberEucalyptus Bio Oct 2020 - PresentIndependent Scientific Advisory Board member.
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Scientific Advisory Board MemberCatalytic Data Science Jul 2020 - PresentCharleston, South Carolina, UsIndependent Scientific Advisory Board member. -
Chief Scientific Officer (Cso)Excision Biotherapeutics Jul 2021 - Nov 2023San Francisco , Ca, UsExcision is developing CRISPR-based therapies to cure viral infectious diseases and improve the lives of chronically ill patients. Excision EBT-101 clinical trial for HIV was awarded Fast Track designation and continues to enroll. Pipeline projects include HIV, HBV, and HSV. https://www.Excision.Bio/ -
Strategic AdvisorGene Editing Frontiers Llc Jan 2020 - Feb 2021Consultant & Advisor on Gene Editing, Gene & Cell Therapy.CRISPR, TALENS, Zinc Finger Nucleases. Bioinformatics & Computational Biology. Strategy, experimental design, due diligence, IP. -
Head Of Genome EditingCrispr Therapeutics Mar 2015 - Nov 2019Boston, Ma, UsHead of Genome Editing and 2nd U.S. employee. CRISPR Therapeutics is translating CRISPR-Cas9, a breakthrough genome-editing technology, into transformative medicines to cure human diseases. Mutations in genes that are causing diseases are specifically targeted by CRISPR-Cas9 and repaired using the cellular repair machinery. Research in the Genome Editing group centers on nuclease activity while improving and ensuring specificity.Highly purchased book chapter published: Cellular Therapies: Gene Editing and Next-Gen CAR T Cells (2016) Novel Immunotherapeutic Approaches to the Treatment of Cancer, 203-247. -
Director Of Protein Engineering FacilityGeorgia Institute Of Technology Apr 2011 - Mar 2015Atlanta, Georgia , Us♦ Developed CRISPR gene editing technologies, including methods to characterized nuclease specificity to drive further improvements. This research resulted in 21 publications and book chapters (so far), plus several patents (see below).♦ Developed and patented exhaustive CRISPR on- and off-target search method, including mismatches and /or differences in length between guide RNA and genomic DNA, termed bulges or gaps. COSMID software is an examples that employs this method.♦ Developed bioinformatic systems for biochemical modeling of protein binding and assisting with protein and experimental design, including TALEN design (SAPTA) and off-target analysis (PROGNOS and COSMID https://crispr.bme.gatech.edu/)♦ Collaborated (within Georgia Tech & Emory, and outside) on targeting genetic mutations using nucleases that we designed.♦ Engineered Zinc Finger Nucleases, TAL Effector Nucleases (TALENs), and CRISPR/ Cas systems and tested hundreds of nucleases, assaying their specificity and modeling their off-target cleavage events.♦ Developed assays and vector systems for improved protein design and high-throughput protein selections or evaluation (See book chapters). -
Biotech / Patent ConsultantBiotechnology Consulting 2010 - 2011Molecular and cell biology consulting (genetics, recombination, cloning and expression systems, assay development) for local and international clients.
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Co-Owner, Scientific AdvisorProtein Delivery Startup 2009 - 2010♦ Started as consultant, then given equity.♦ Advised on molecular and cellular biology methods and technology, protein engineering, literature, and filings for a biotech startup company focused on gene and gene therapy delivery.♦ Wrote declaration and contributed to the patent filings.
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Postdoctoral Fellow, Zinc Finger Nuclease Design & TestingUniversity Of Iowa 2009 - 2010Iowa City, Iowa, UsDeveloped 14 sets of validated Zinc Finger Nucleases to create disease models in Cell Lines, Rat, Mice and Zebrafish.Set up cloning for Viral Vectors for our Zinc Finger Nucleases.Developed Bioinformatics and web interface that designs Zinc Finger Nucleases that target user-specified gene segments.ZFN-Site searches genomes for zinc finger nuclease target sites and off-target sites. TJ Cradick, Ph.D., Giovanna Ambrosini, Christian Iseli, Philipp Bucher, Anton P McCaffrey BMC Bioinformatics 2011, 12:152 http://www.biomedcentral.com/1471-2105/12/152ZFN-Site is a web interface that searches multiple genomes for ZFN off-target sites. Queries can be based on the target sequence or can be expanded using degenerate specificity to account for known ZFN binding preferences. ZFN off-target sites are outputted with links to genome browsers, facilitating off-target cleavage site screening.CL Ramirez, MT Certo, C. Mussolino, MJ Goodwin, TJ Cradick, AP McCaffrey, T. Cathomen, AM Scharenberg, JK Joung.Engineered zinc finger nickases induce homology-directed repair with reduced mutagenic effects. Nucleic Acids Research. 2012, 1-9. -
Phd Student To Post Doc, Zinc Finger Nuclease Design, Testing And DevelopmentUniversity Of Iowa 2004 - 2009Iowa City, Iowa, UsDeveloped Zinc Finger Nucleases (ZFNs) that specifically cleave Hepatitis B Viral (HBV) DNA. Developed ZFN-site bioinformatic tool to help design zinc finger proteins and scan genomes for possible on-target and off-target sites. Developed assays to measure cleavage and mis-repair of ZFN sites and off-target sites:One of two groups to develop and first present the Surveyor assay using Cel-1.Developed biochemical methods and kinetics assay to measure activity and specificity of nucleases, including a streamlined in vitro cleavage assay.TJ Cradick, K Keck, S Bradshaw, AC Jamieson and AP McCaffrey. Zinc-Finger Nucleases as a Novel Therapeutic Strategy for Targeting Hepatitis B Virus DNAs. Molecular Therapy, 2010, May;18(5):947-54 -
Associate Scientist Ii, Zinc Finger Design & Selection GroupSangamo Biosciences 2000 - 2004Brisbane, California, Us•Part of Zinc Finger and Zinc Finger Nuclease design (protein engineering) and selection group charged with producing DNA-binding domains with high activity and specificity.•Developed new in vitro display selection technologies. Selected and tested ZFPs using Bacterial Two-Hybrid, SELEX and other systems.•Chose genomic targets using Bioinformatics, Southern Analysis and DNase Hypersensitivity Mapping.•Constructed artificial transcription factors with ZFPs fused to Activation and/or Repression Domains, transfected cells, and prepared RNA for analysis. Improved standard protocols and help troubleshoot these processes.•Designed and optimized Taqman Primers and Probes and quantitated resulting changes in mRNA expression using Taqman and Affymetrix. Further analyzed the role our molecules played in gene regulation using Bioinformatic methods.•Biochemical studies of ZFPs, including Gel-Shift and Off-Rate experiments and Alanine Screens.AC Jamieson, Bo Guan, TJ Cradick, H Xiao, PD Gregory and PM Carroll. Controlling gene expression in transgenic Drosophila embryos using engineered zinc finger protein transcription factors. Biochemical and Biophysical Research Communications, 2006, Sep. 29, 348(3): 873-9. -
Phage Display For High-Throughput ScreeningTularik 1998 - 1999UsDesigned and developed a novel series of phage display libraries for use in high-throughput cellular assays. -
Phd Student In Immunology And Microbiology, Developing Proteolytic AntibodiesUniversity Of California, San Francisco 1993 - 1998San Francisco, California, Us•Thesis: Selection of Proteolytic Antibodies Against HIV gp120 (Advisor, Dr. Matthias Wabl). •Developed Novel Phage-Based Selection for proteolytic antibodies. Tested via HPLC.•Devised method to screen very large Antibody Libraries: •Cloned Very Large Phage Display Antibody Libraries, both naïve and from mice I immunized with transition-state analogs.•Assisted with troubleshooting email-based querying of Kabat Immunologic gene database for V-region primer design and other projects. -
Immunology Consultant For Law FirmRopers, Majeski, Kohn & Bentley 1996 - 1996•Immunology consultant for law office handling cases of individuals with suspected autoimmune responses to breast implants.•Advised on literature, methods, current research, and possible further experimentation.
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Research Associate, Hiv Reseach, Phage Display & Drug DiscoveryRepligen Corporation 1989 - 1993Waltham, Ma, Us•Brought in-house, developed and optimized a phage display library system and wrote SOPs for mapping the binding domains of anti-HIV antibodies and cell surface proteins. Screened phage displayed peptide libraries for use in small molecule drug discovery focused on the B7 /CD28 /CTLA4 interactions.•Engineered and expressed fusion proteins, and developed a phage expression system for structure/function analysis of protein domains for small molecule drug discovery.•Prepared HIV clones using Long PCR for syncitia formation and neutralization assays.CL Jellis, TJ Cradick, P Rennert, P Salinas, J Boyd, T Amirault and GS Gray. Defining critical residues in the epitope for a HIV-neutralizing monoclonal antibody using phage display and peptide array technologies. Gene, 1993 Dec. 27, 137(1):63-8. -
Research Associate, Diabetes Research, Molecular Biology LabJoslin Diabetes Center 1988 - 1989Boston, Massachusetts, UsMay 1988 - Oct. 1989•Effects of SV40 large T and small t antigens on transcriptional control.•Operation of, and training others for use with the molecular core facility.•Rebuilt and operated the oligo. synthesizer and assisted others with PCR primer design. -
Campus Tour GuideMit 1985 - 1988Cambridge, Ma, Us -
Urop Undergraduate ResearcherMit 1986 - 1986Cambridge, Ma, UsTested polymers for drug delivery in Robert Langer's Laboratory.
Tj Cradick, Ph.D. Skills
Tj Cradick, Ph.D. Education Details
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University Of IowaMolecular And Cellular Biology -
University Of California, San FranciscoImmunology -
Massachusetts Institute Of TechnologyLife Sciences -
Queen Mary University Of LondonPart Of The University Of London. -
Marist School
Frequently Asked Questions about Tj Cradick, Ph.D.
What company does Tj Cradick, Ph.D. work for?
Tj Cradick, Ph.D. works for Gene Editing Frontiers Llc
What is Tj Cradick, Ph.D.'s role at the current company?
Tj Cradick, Ph.D.'s current role is CSO and Principal.
What is Tj Cradick, Ph.D.'s email address?
Tj Cradick, Ph.D.'s email address is tj****@****rtx.com
What schools did Tj Cradick, Ph.D. attend?
Tj Cradick, Ph.D. attended University Of Iowa, University Of California, San Francisco, Massachusetts Institute Of Technology, Queen Mary University Of London, Marist School.
What are some of Tj Cradick, Ph.D.'s interests?
Tj Cradick, Ph.D. has interest in Etc, Bioinformatics, Talen And Zinc Finger Nucleases, Crispr, 2 Hybrid, Bbq, Genomics.
What skills is Tj Cradick, Ph.D. known for?
Tj Cradick, Ph.D. has skills like Cell, Molecular Biology, Protein Chemistry, Biochemistry, Pcr, Drug Discovery, Laboratory, Immunology, Assay Development, Genetics, Biotechnology, Bioinformatics.
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