Hired at a newly minted antibody discovery CRO to assist with the current antibody discovery capabilities, expand the current capabilities, and play an active role in business development.

Managed a cross-functional team of scientists to implement an immunization program of mice to produce specific antibodies and to develop a single cell cloning system to isolate specific antibodies. Performed ELISAs and flow cytometry to find specific antibodies that would bind to the target antigen in the serum from immunized mice. Expressed soluble proteins and GPCRs in suspension HEK cells at scales ranging from 5 mls to 6L. Implemented a system for producing lentiviruses to create stable… Show more Managed a cross-functional team of scientists to implement an immunization program of mice to produce specific antibodies and to develop a single cell cloning system to isolate specific antibodies. Performed ELISAs and flow cytometry to find specific antibodies that would bind to the target antigen in the serum from immunized mice. Expressed soluble proteins and GPCRs in suspension HEK cells at scales ranging from 5 mls to 6L. Implemented a system for producing lentiviruses to create stable cell lines. Show less

Expressed antibodies and nanobodies in suspension HEK cells at a scale of 50 mls-3L. Purified antibodies and nanobodies using protein A chromatography on an AKTA system. Involved in designing and implementing an automated approach for small scale purification of proteins. Managed a team of scientists that expressed and purified proteins to support other groups in the company.

Analyzed and re-tooled the in-house HCP ELISA for product release including purifying and characterizing new batches of HCP antibodies and developing ways to increase the robustness of the assay. Performed analytical analysis (SDS-PAGE, RP-HPLC, SEC and 2D-PAGE, and 2D-PAGE western blots) in support of experiments.

Designed and executed experiments in support of the commercial manufacturing process including development, scale up, troubleshooting, and deviations for both upstream and downstream processes. Provided support of protein purification, isolation, and characterization. Identified and supported improvements to the commercial manufacturing process, focusing on increased product yield or quality improvements, increased efficiency or simplification of operations, risk mitigation, and reduced cycle… Show more Designed and executed experiments in support of the commercial manufacturing process including development, scale up, troubleshooting, and deviations for both upstream and downstream processes. Provided support of protein purification, isolation, and characterization. Identified and supported improvements to the commercial manufacturing process, focusing on increased product yield or quality improvements, increased efficiency or simplification of operations, risk mitigation, and reduced cycle time. Collaborated with manufacturing technology, manufacturing and quality control, R&D, validation, and quality assurance on projects and initiatives. Show less

Led ten campaigns to produce monoclonal antibodies specific for glyco-peptides. Managed CROs for peptide production and animal immunizations. Developed CRISPR protocols for gene knockout cells. Worked directly with the company president and CSO to develop the company’s scientific strategy.

Introduced the technology of expressing recombinant antibodies to the DFCI MAC and the ability to class switch antibodies using recombinant techniques.

Produced milligram scale amounts of proteins including antibodies for over 100 projects in oncology, gastroenterology, and immunology using affinity, ion-exchange and hydrophobic interaction chromatography on an AKTA HPLC.

Managed and provided customer service for over 50 antibody generation projects in many therapeutic areas, i.e., oncology, neurobiology, and microbiology. Handled external work of CROs including extensive interactions with technical and administrative staff. Screened hybridomas for specific antibody production by ELISA, immunoblotting, FACS, or productivity for over 60 different projects in oncology, neurobiology, and microbiology.

Developed a novel system for tracking RNA in cells infected with retroviruses, a class of viruses that includes HIV. Characterized the contents of the viral particles from a newly discovered human retrovirus. Directly supervised research technicians and new graduate students in the lab. Lead scientist from Tufts University in a consortium on Retroviral RNA that included groups from Johns Hopkins University and the University of Washington. Consortium presented results at a roundtable discussion… Show more Developed a novel system for tracking RNA in cells infected with retroviruses, a class of viruses that includes HIV. Characterized the contents of the viral particles from a newly discovered human retrovirus. Directly supervised research technicians and new graduate students in the lab. Lead scientist from Tufts University in a consortium on Retroviral RNA that included groups from Johns Hopkins University and the University of Washington. Consortium presented results at a roundtable discussion at the annual retroviral meeting. Reviewed over 20 journal articles for Cell, Science, Nature and the Journal of Virology. Wrote journal articles and reviewed grants for submission to the NIH. Show less

Developed an ex vivo gene therapy approach for the treatment of Parkinson's disease by engineering a cell line to produce L-DOPA and dopamine using retroviral vectors. Successfully grafted dopamine expressing cell lines into rodent models for PD, which led to significant improvement in the animal’s disease state. Co-wrote an RO1 to help fund the lab. Set up the lab of a new investigator.