- Performs in vivo drug efficacy studies in murine immuno-compromised and immuno-competent cancer models - Stains, runs, and analyzes flow cytometry (FACS) studies for T cell count, phenotyping, and target expression in blood, tumors, spleens, and in culture- Executes and analyzes serum MSD assays for in vivo cytokine evaluation- Cultures large quantities of cells (ex. 30 T225s) for in vivo, in vitro, and mass spectroscopy studies- Presents project data to peers- Active participant in two internal technology development committees- Created an inventory documentation system for banked frozen samples- Authored detailed protocols for future use by group members

- Acts as the Lead Good Laboratory Practices (GLP) Research Technician in collaboration with the GLP Director to organize and execute FDA stage 2 preclinical in vivo studies for efficacy and toxicity- Utilizes the Laboratory Information Management System and Standard Operating Procedures (SOPs)- Generates and maintains an in vivo and frozen bank of over 170 different Patient Derived Xenografts (PDXs)- Experienced in PO, IV, SQ, trocar SQ, IP, IC, IO, RO, OT pancreas and mammary fat pad injections- Resects and prepares tissue by necropsy, fixing (formalin, OCT, and PFA), trimming, embedding, sectioning, and staining- Collects blood via tail, RO, and cheek bleeds, runs CBC readings, and performs bone marrow aspirates for bone marrow collection- Maintains the Heska HemaTrue and Heska HT5 chemistry analyzers- Calculates and prepares drug concentrations in vehicle solutions/suspensions- Performs tumor measurements via calipers, palpation, and IVIS imaging with bioluminescence and fluorescence- Performs survival surgeries for OT pancreas and mammary fat pad injections utilizing Ketamine/Xylazine, sutures, and staples- Tissue collection and preparation by GentleMACS enzymatic dissociation, flash freezing, and viable seed freezing

- Planned and executed in vivo metastatic and primary allograft and xenograft tumor studies with immunocompetent and immunocompromised mice for pharmaceutical anticancer research- Trained in mouse handling techniques including intravenous (IV), intraperitoneal (IP), subcutaneous, and intradermal injections- Dissected mice, measured the biodistribution of drug reagents or cancer cells within specific organs, and preserved tissues for future slide preparation- Decellularized mouse lung and liver tissue to study cell viability in 3D culture using MTT assays- Synthesized porous PLGA 3D scaffolds for cell culture- Performed IC50 studies of breast cancer cells treated with doxorubicin- Mentored a highschool student in cell culture technique and constructing a monoclonal cell line- Tested the efficacy of targeted drug delivery vehicles in in vivo primary and metastatic mouse models by measuring fluorescence and bioluminescence with a PerkinElmer IVIS Lumina II- Constructed liposomal drug delivery vehicles with cancer targeting antibodies- Cultured, spilt, and froze murine breast cancer cells- Transfected cells with a bioluminescent gene using lipofectamine- Performed flow cytometry experiments utilizing antibodies and microbeads with a LSR II- Modeled applied forces on a hydrogel trachea scaffold in SolidWorks Simulation- Synthesized hydrogel trachea scaffolds and analyzed them with a tensile tester

- Analyzed over 4,000 patent applications pertaining to surgical instruments, therapies, medical prostheses, and composite materials by interpreting technical drawings, examining technical writing, comparing to past patents, and scrutinizing the inventive concept- Led the Surgical/Medical Collaborative Work-group in composing and presenting topics of discussion on the international Collaborative Patent Classification system with the United States Patent and Trademark Office (USPTO)- Appointed subject matter expert in electrical stimulation therapy, surgical manipulation, and prosthetic devices- Elected by fellow coworkers to represent the department team in round table meetings with the Vice President and other department representatives

- Interpreted blueprint and project drawings to determine material and labor requirements- Ordered materials from suppliers, maintained inventory, and coordinated deliveries

- Named the top RHA in the northeast region out of the 45 schools affiliated with NEACURH- Negotiated initial conversations with USG to increase the RHA budget by an additional $40,000- Exercised leadership skills including conflict resolution, goal setting, delegating, and motivation

- Analyzed multiple MS-Excel files by creating histograms, scatter plots, macros, and normalizing data- Designed and constructed a prototype external power supply system to be used at locomotive crash sites by researching and purchasing the materials needed, designing a wiring diagram, soldering the devices and wires together, and testing the system using a multimeter- Developed business model proposals for the development of Locomotive Interface Gateway software- Reviewed and authored an evaluation of GE’s response to the Association of American Railways’ RFI- Co-facilitated a silent auction to raise money for the United Way of Brevard County

- Installed heat and frost insulation at the Corning Glass plant in Canton, Anheuser-Busch bottling plant in Baldwinsville, Solvay Paper Board in Solvay, and Northeast Biofuels ethanol plant in Fulton, New York- Certified in safety training courses for working in a manufacturing environment

- Synthesized medium for plankton cultures with the use of pipettes and an autoclave- Assisted in trace metal grade acid baths for sterilization of lab equipment

- Estimated, negotiated, and fulfilled landscaping contracts

- Directed traffic during road construction

- Trained Crew Members on and held Drive-Thru, Grill, Assembly, Runner, and Order Taking positions