Crystal Gregory Email & Phone Number

Fort Thomas, Kentucky, United States

Independence, Kentucky, United States

•Prepare human brain tissue homogenates, and analyze samples via Western blot.•Organize and manage the IBC protocol, ACUP protocol, Carlson Biosafetyanimal colony, and CIS facilities mouse colony management.•Data Analysis and data preparation for publication and grants.•Oversee and train students in laboratory safety and experimental technique.•Prepare human and mouse tissue homogenates for experiments and Westernblot analysis.•Use of scrapie cell culture system to predict the scrapie infectivity of differentmutations in prion protein.•Use of fluorescence resonance energy transfer to predict altered molecularassociation of prion protein in prion disease.•Drug Intraperitoneal injections into mice for ongoing experiments.•Patient DNA extraction from blood and human tissue; followed by DNA sequenceanalysis to genetically screen for Creutzfeldt-Jakob disease.•Preparation of 300 μm and 700 μm mouse brain sections using a vibratome withRinger solution, carbogen (95% O2/5%CO2), and ice•Gonadotropin releasing hormone (GnRH) neuron secretion analyses areperformed in the presence of veratridine, which opens Na+channels causingdepolarization of the neuron. Then analysis is done via radioimmunoassay (RIA)using GnRH antibody radiolabeled with I125 to quantify the amount of hormonebeing released by the neuron.•Protein purification using baculovirus expression system and pMAL proteinfusion and purification system.•Organized and maintained Neuroblastoma, SMB, HEK and COS7 cell culture•Bacterial and yeast transformation, molecular cloning, DNA sequencinganalysis, UV Mutagenesis, gene disruption, site directed mutagenesis, Westernblots, and enzyme kinetic assays.•DNA purification, Restriction Enzyme digest, PolymeraseChain Reaction, and RT-PCR•Experience with tissue fixation, embedding, histology, operation of thetransmission electron microscope (TEM) and Confocal Microscope.•ELISA and antibody purification, NMR, and IR spectroscopy

Master'S Degree, Clinical Research Coordinator, 3.8/4.0 Scale

Bs

Bs, Premedicine, Minor Chemistry