•Prepare human brain tissue homogenates, and analyze samples via Western blot.•Organize and manage the IBC protocol, ACUP protocol, Carlson Biosafetyanimal colony, and CIS facilities mouse colony management.•Data Analysis and data preparation for publication and grants.•Oversee and train students in laboratory safety and experimental technique.•Prepare human and mouse tissue homogenates for experiments and Westernblot analysis.•Use of scrapie cell culture system to predict the scrapie infectivity of differentmutations in prion protein.•Use of fluorescence resonance energy transfer to predict altered molecularassociation of prion protein in prion disease.•Drug Intraperitoneal injections into mice for ongoing experiments.•Patient DNA extraction from blood and human tissue; followed by DNA sequenceanalysis to genetically screen for Creutzfeldt-Jakob disease.•Preparation of 300 μm and 700 μm mouse brain sections using a vibratome withRinger solution, carbogen (95% O2/5%CO2), and ice•Gonadotropin releasing hormone (GnRH) neuron secretion analyses areperformed in the presence of veratridine, which opens Na+channels causingdepolarization of the neuron. Then analysis is done via radioimmunoassay (RIA)using GnRH antibody radiolabeled with I125 to quantify the amount of hormonebeing released by the neuron.•Protein purification using baculovirus expression system and pMAL proteinfusion and purification system.•Organized and maintained Neuroblastoma, SMB, HEK and COS7 cell culture•Bacterial and yeast transformation, molecular cloning, DNA sequencinganalysis, UV Mutagenesis, gene disruption, site directed mutagenesis, Westernblots, and enzyme kinetic assays.•DNA purification, Restriction Enzyme digest, PolymeraseChain Reaction, and RT-PCR•Experience with tissue fixation, embedding, histology, operation of thetransmission electron microscope (TEM) and Confocal Microscope.•ELISA and antibody purification, NMR, and IR spectroscopy