Crystal Lee Email & Phone Number

Novato, California, United States

Upstream PD: small scale bioreactor work to optimize production of therapeutics (gene therapy and ERT).

Novato, CA

Cell Culture Process Development

-Purified antibodies for in-vitro assays on Protein G and Protein A columns for in-vitro assays (15ml to 3L) via syringe barrel and AKTA Avant.-Screened hydridoma cells via ELISA and utilized Agilent Bravo liquid handler to work with cultures in 384-well format. -Subcloned hybridoma by limited dilution method and selected high producers by screening via.

San Francisco Bay Area

Investigate the activation domain of IRE1 in mammalian cells by reconstituting GFP-tagged IRE1 truncation mutants into knock out MEF cell lines using lentiviral transduction. Assist in a high throughput screen for genetic vulnerabilities of multiple myeloma cells in vitro in combination with small molecule inhibitors of the unfolded protein response.

San Francisco Bay Area

Investigate IRE1 in the Peter Walter lab at UCSF.

San Francisco Bay Area

San Francisco Bay Area

• Maintain and subculture mammalian suspension cell cultures for in-house use and analysis

UCSF Mission Bay Campus, San Francisco, CA

San Francisco Bay Area

• Certificate Programs in Stem Cell Technology and Biomanufacturing
• Produced/purified green fluorescent protein utilizing E. coli in small scale bioreactor under cGMP conditions
• Utilized pichia pastoris expression system and the AOX1 promoter to produce human serum albumin
• Cell culture of adherent cell lines CHOK1, COS7, UV5, 3T3 and analysis with fluorescent tags
• Induced differentiation of pluripotent C2C12 and E14 cell lines into cardiomyocytes, neurons, and myosin and analysis using fluorescence microscopy

Santa Cruz, CA

• Lab Assistant II, Monoclonal Antibodies (Jan 2009 to Sept 2009)
• Performed QC of antibodies by Western Blot.
• Processed and aliquoted product for distribution. Lab Assistant I, Monoclonal Antibodies (Jun 2008 to Jan 2009)
• Performed initial extraction of crude protein from mammalian cells.
• Prepared buffers and reagents following SOPs.
• Organized and kept stock of laboratory supplies.

Certificate, Stem Cell Technology/Biomanufacturing

Relevant Coursework: Mammalian Cell Culture, Fluorescent Cell Technology, Stem Cell Technology, Fermentation and Protein Purification.

Bachelor Of Science, Chemistry

Relevant Coursework: Instrumental Analysis, Organic/Inorganic/Physical Chemistry, Advanced Organic Chemistry Lab