Christopher Triana Email & Phone Number

Madison, Wisconsin Area

Greater San Diego Area

Research and Development of HBV assay for the Panther system - Diagnostics Division. Specialization in assay development for the Panther, a fully automated chemistry and immunoassay instrument. Assay development in the Diagnostics Division for HBV detection in blood, serum and plasma. Experience in Feasibility testing, Formulation review, Validation and Verification and Assay Integration in a GLP and QSR (cGMP) lab environment. • Knowledge and experience of proprietary technologies involving RT-PCR, specifically Transcription Mediated Amplification (TMA) and Hybridization Protection Assay/Dual Kinetic Assays (HPA)• Research and develop a quantitative and qualitative NAT assay (HBV)• Design and execute experiments to complete a segment of overall project for formulation studies.• Experiment with different combinations of oligos, target capture, amplification and hybridization to maximize the assay sensitivity while maintaining its accuracy. • Analyze data and troubleshoot any problematic aspects of the assay.• Develop simple and complex assay protocols to demonstrate feasibility of the assay. • Qualify and test new software to identify problems or bugs• Test clinical samples for infected viruses such as; HBV using the Panther automated machine. • Manage project’s workload, organized small meetings and updates on project progress. Inspect and manage lab safety and inventory supplies. • Present monthly presentations and reports at group meeting. • Train new hires for usage of the automated Panther machine.

La Jolla, San Diego

Purify and analyze multiple protein constructs and protein-ligand complexes for use in protein crystallization (GPCR's) and structure based drug design. Also assisted in method development, improving current laboratory practices, general organization, and managerial necessities within the lab. Structural Biology • Experience with HPLC (Dionex : Ultimate 3000 Tandem) • Mass Spectroscopy (Applied Biosystems – 3200 Q TRAP) • Fluorescence Spectroscopy • UV and IR Spectroscopy • Membrane Protein Purification • Lipidic Cubic Phase Preparation • Lyophilization • Sonication • Proficient with operating automation and screening platforms, including multiple plate formats (96 well)Molecular Biology • DNA Purification Methods such as Mini preparation of Plasmids, PCR Purification, Gel Purification • Ligation • Transformation • Digestion

Mira Mesa; West View Parkway

• Work in a fast-paced work environment while gaining hands-on experience with customer service and customer satisfaction• Develop sales techniques and abilities• Providing excellent customer service• Use of effective communication skills

Greater San Diego Area

Carried out research related to the study of iron biogeochemistry in marine microflora, and the cause and effect on algal blooms. This required extensive proficiency in DNA and RNA extraction, protein purification methods and work with bioinformation tools such as Geneious and the National Center of Biotechnology Information (NCBI).• Can efficiently perform DNA and RNA extractions and the Polymerase Chain Reaction. Additionally, Real Time Polymerase Chain Reaction/Reverse Transcriptase PCR• Experience with column chromatography (chelexing complexes, nickel affinity, and size exclusion chromatography). • Proficient on gel electrophoresis and SDS gels• Proficiency in Plasmid isolation, transformations, cloning and protein expression• Experience with bio-information software tools: Vector NTI, Geneious, and National Center of Biotechnology Information (NCBI).

Bachelor Of Science (B.S.), Biochemistry

Pursuing B.S. In Biochemistry, Chemistry

Education record

Go Mustangs!

Education record