Cynthia Millard

Cynthia Millard Email and Phone Number

Research Scientist
Cynthia Millard's Location
Portland, Oregon Metropolitan Area, United States
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About Cynthia Millard

Masters level biotechnology professional with over 25 years of experience in laboratory research including expertise in microbiology, proteomics, environmental research, molecular biology, and analytical chemistry (HPLC). I am a creative individual with a deep work ethic, a passion for knowledge, and a focus on quality. I have contributed to institutions through method development, organization, and enhanced efficiency.My Career Highlights Include:•Oregon Bioscience (OBA) Medical Devices Foundations Certificate - 80 classroom-hours including Quality Systems, Project management, and Statistical Data Analysis.•Manufacture and QA of MHC tetramers for detection of antigen specific T-cells in an ISO regulated environment (MBL).•Managed protein crystallization, structure project that resulted in >$150K in achieved milestones (deCODE).•Discovered E.coli mutant that produces >90% succinic acid by anaerobic fermentation and resulted in 4 patents with >$500k in royalties (Argonne Nat Lab)•Developed, manufactured and marketed product line of luminometry reagents (Packard Instruments).Core Capabilities: •Classical microbiology techniques - Aerobic & Anaerobic culture and CSTR fermentation.•Protein expression and purification using AKTA systems•Protein crystallization screens •Spectrometric Enzyme Assays•DNA cloning•DNA and Protein electrophoresis gels •PCR•HPLC - organic acids, sugars, amines, cations and anions, ethanol, and vitamins•Luminometry assays - cell viability assay, neutrophil function, ATP & NADH based assays.

Cynthia Millard's Current Company Details

Research Scientist
Cynthia Millard Work Experience Details
  • Purity Labs
    Senior Scientist
    Purity Labs Jan 2015 - Aug 2015
    Lake Oswego, Or
    HPLC (Shimadzu) analysis of nutraceuticals including vitamins A, D, E, Niacin, Thiamine, Pyridoxine, Riboflavin, Pantothenate, and Biotin. Microbial analysis of food, environmental samples, and nutraceuticals.
  • Mbl International Corporation
    Laboratory Scientist
    Mbl International Corporation May 2013 - Apr 2014
    Desplaines, Il
    Manufacturing and quality assurance (QA) of Class I & II MHC tetramers (for detection of specific T-cell receptors) in an ISO regulated environment. Manufactured Class I and Class II tetramers (for sensitive detection of specific T-cell receptors) using inclusion body expression (B2M, monomer), monomer extraction and re-folding, protein concentration, protein purification, HPLC quantitation of monomers, biotinylation and avidin purification, spectrophotometry and QC analysis of intermediate… Show more Manufacturing and quality assurance (QA) of Class I & II MHC tetramers (for detection of specific T-cell receptors) in an ISO regulated environment. Manufactured Class I and Class II tetramers (for sensitive detection of specific T-cell receptors) using inclusion body expression (B2M, monomer), monomer extraction and re-folding, protein concentration, protein purification, HPLC quantitation of monomers, biotinylation and avidin purification, spectrophotometry and QC analysis of intermediate and final products. Show less
  • Argonne National Laboratory
    Microbiologist
    Argonne National Laboratory Nov 2012 - May 2013
    Argonne, Il
    • Developed and implemented detailed protocol for screening halophiles and thermophiles for ability to plug pores in oil formation reservoirs to enhance oil recovery. • Researched literature and prepared a draft report on microbial forensics analysis techniques.
  • Argonne National Laboratory
    Microbiologist, Energy Systems
    Argonne National Laboratory Nov 2009 - Feb 2012
    9700 S. Cass, Argonne, Il 60439
    • Integrated succinic acid fermentation and purification using EDI (electrodeionization)• cultured and tested clostrida species for production of ethanol from syngas• tested enzyme immobilization techniques for carbonic anhydrase for carbon dioxide capture project• produced GFOR enzyme using Zymomonas mobilis anaerobic fermentations for EDI experiments.• HPLC analysis of sugars, organic acids, ethanol, cations/anions, and amines.
  • Decode Biostructures (Now Beryllium)
    Research Scientist
    Decode Biostructures (Now Beryllium) Apr 2005 - Nov 2009
    Woodridge, Il
    • Project leader for antibiotic bacterial protein target with 13 co-crystallized ligand structures. E. coli fermentations (2-10L) for protein expression. • Optimized protein expression. • Protein purification on AKTA systems. • Crystal Screening and Freezing crystals. •Western Blot. Dot blot. •Page gels.
  • Argonne National Laboratory
    Microbiologist, Environmental And Biology Research
    Argonne National Laboratory Aug 1988 - Apr 2005
    Argonne, Il
    • Small scale Fermentation (1-4L).• Aerobic and anaerobic bacterial growth. • Spectrophotometric enzyme assays.• DNA mini and midi purification preps. • Restriction digests. • Made competent cells. • Transformation and Transduction. • SDS-Page gels. DNA gels.• Generated unique bacterial strain that produces succinic acid • Expression of proteins in E. coli for protein structure determination.• Improved throughput for protein production for… Show more • Small scale Fermentation (1-4L).• Aerobic and anaerobic bacterial growth. • Spectrophotometric enzyme assays.• DNA mini and midi purification preps. • Restriction digests. • Made competent cells. • Transformation and Transduction. • SDS-Page gels. DNA gels.• Generated unique bacterial strain that produces succinic acid • Expression of proteins in E. coli for protein structure determination.• Improved throughput for protein production for proteomics using disposable two liter plastic bottles. • Produced selenomethionine labeled proteins for structure determination.• Developed method for enzymatically converting starch waste streams to glucose. • Used HPLC for analysis and quantitation of fermentation products. Show less
  • Joliet Junior College
    Biology Laboratory Instructor
    Joliet Junior College Sep 1998 - Jun 1999
    Joliet, Il
    Instructor for lecture and lab class including cell biology, chemistry, genetics, photosynthesis, metabolism.
  • Los Alamos Diagnostics
    Research And Industrial Products Manager
    Los Alamos Diagnostics Oct 1986 - Mar 1989
    Los Alamos, Nm
    • Generated contacts and collaborated with universities and industry to develop methods for rapid detection of microorganisms, cell viability, and other applications of luminescent reactions. • Produce literature and generated strategies to promote reagents and instrumentation.
  • Packard Instruments (Now Part Of Perkinelmer, Inc.)
    Applications Microbiologist
    Packard Instruments (Now Part Of Perkinelmer, Inc.) Aug 1978 - Oct 1986
    Downers Grove, Il
    • Tested and documented applications, reagents, and equipment based on luminescent reactions. • Presented luminescent methods at professional conferences and customer demos.• Defined reagent production methods and QA procedures using GMP and GLP.

Cynthia Millard Skills

Protein Purification Protein Expression Hplc Cell Culture Protein Chemistry Microbiology Dna Molecular Biology Biotechnology Life Sciences Purification Western Blotting Chromatography Pcr Laboratory Gel Electrophoresis Bacterial Culture Method Development Fermentations Dna Purification Laboratory Skills

Cynthia Millard Education Details

Frequently Asked Questions about Cynthia Millard

What is Cynthia Millard's role at the current company?

Cynthia Millard's current role is Research Scientist.

What is Cynthia Millard's email address?

Cynthia Millard's email address is cs****@****hoo.com

What is Cynthia Millard's direct phone number?

Cynthia Millard's direct phone number is (630) 252*****

What schools did Cynthia Millard attend?

Cynthia Millard attended Illinois Institute Of Technology, Lewis University, University Of Illinois At Urbana-Champaign.

What are some of Cynthia Millard's interests?

Cynthia Millard has interest in Animals, Art, Reading, Science Fiction, Health, The Environment, Children, Biotechnology, Sewing, Environment.

What skills is Cynthia Millard known for?

Cynthia Millard has skills like Protein Purification, Protein Expression, Hplc, Cell Culture, Protein Chemistry, Microbiology, Dna, Molecular Biology, Biotechnology, Life Sciences, Purification, Western Blotting.

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