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Masters level biotechnology professional with over 25 years of experience in laboratory research including expertise in microbiology, proteomics, environmental research, molecular biology, and analytical chemistry (HPLC). I am a creative individual with a deep work ethic, a passion for knowledge, and a focus on quality. I have contributed to institutions through method development, organization, and enhanced efficiency.My Career Highlights Include:•Oregon Bioscience (OBA) Medical Devices Foundations Certificate - 80 classroom-hours including Quality Systems, Project management, and Statistical Data Analysis.•Manufacture and QA of MHC tetramers for detection of antigen specific T-cells in an ISO regulated environment (MBL).•Managed protein crystallization, structure project that resulted in >$150K in achieved milestones (deCODE).•Discovered E.coli mutant that produces >90% succinic acid by anaerobic fermentation and resulted in 4 patents with >$500k in royalties (Argonne Nat Lab)•Developed, manufactured and marketed product line of luminometry reagents (Packard Instruments).Core Capabilities: •Classical microbiology techniques - Aerobic & Anaerobic culture and CSTR fermentation.•Protein expression and purification using AKTA systems•Protein crystallization screens •Spectrometric Enzyme Assays•DNA cloning•DNA and Protein electrophoresis gels •PCR•HPLC - organic acids, sugars, amines, cations and anions, ethanol, and vitamins•Luminometry assays - cell viability assay, neutrophil function, ATP & NADH based assays.
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Senior ScientistPurity Labs Jan 2015 - Aug 2015Lake Oswego, OrHPLC (Shimadzu) analysis of nutraceuticals including vitamins A, D, E, Niacin, Thiamine, Pyridoxine, Riboflavin, Pantothenate, and Biotin. Microbial analysis of food, environmental samples, and nutraceuticals. -
Laboratory ScientistMbl International Corporation May 2013 - Apr 2014Desplaines, IlManufacturing and quality assurance (QA) of Class I & II MHC tetramers (for detection of specific T-cell receptors) in an ISO regulated environment. Manufactured Class I and Class II tetramers (for sensitive detection of specific T-cell receptors) using inclusion body expression (B2M, monomer), monomer extraction and re-folding, protein concentration, protein purification, HPLC quantitation of monomers, biotinylation and avidin purification, spectrophotometry and QC analysis of intermediate… Show more Manufacturing and quality assurance (QA) of Class I & II MHC tetramers (for detection of specific T-cell receptors) in an ISO regulated environment. Manufactured Class I and Class II tetramers (for sensitive detection of specific T-cell receptors) using inclusion body expression (B2M, monomer), monomer extraction and re-folding, protein concentration, protein purification, HPLC quantitation of monomers, biotinylation and avidin purification, spectrophotometry and QC analysis of intermediate and final products. Show less -
MicrobiologistArgonne National Laboratory Nov 2012 - May 2013Argonne, Il• Developed and implemented detailed protocol for screening halophiles and thermophiles for ability to plug pores in oil formation reservoirs to enhance oil recovery. • Researched literature and prepared a draft report on microbial forensics analysis techniques. -
Microbiologist, Energy SystemsArgonne National Laboratory Nov 2009 - Feb 20129700 S. Cass, Argonne, Il 60439• Integrated succinic acid fermentation and purification using EDI (electrodeionization)• cultured and tested clostrida species for production of ethanol from syngas• tested enzyme immobilization techniques for carbonic anhydrase for carbon dioxide capture project• produced GFOR enzyme using Zymomonas mobilis anaerobic fermentations for EDI experiments.• HPLC analysis of sugars, organic acids, ethanol, cations/anions, and amines. -
Research ScientistDecode Biostructures (Now Beryllium) Apr 2005 - Nov 2009Woodridge, Il• Project leader for antibiotic bacterial protein target with 13 co-crystallized ligand structures. E. coli fermentations (2-10L) for protein expression. • Optimized protein expression. • Protein purification on AKTA systems. • Crystal Screening and Freezing crystals. •Western Blot. Dot blot. •Page gels.
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Microbiologist, Environmental And Biology ResearchArgonne National Laboratory Aug 1988 - Apr 2005Argonne, Il• Small scale Fermentation (1-4L).• Aerobic and anaerobic bacterial growth. • Spectrophotometric enzyme assays.• DNA mini and midi purification preps. • Restriction digests. • Made competent cells. • Transformation and Transduction. • SDS-Page gels. DNA gels.• Generated unique bacterial strain that produces succinic acid • Expression of proteins in E. coli for protein structure determination.• Improved throughput for protein production for… Show more • Small scale Fermentation (1-4L).• Aerobic and anaerobic bacterial growth. • Spectrophotometric enzyme assays.• DNA mini and midi purification preps. • Restriction digests. • Made competent cells. • Transformation and Transduction. • SDS-Page gels. DNA gels.• Generated unique bacterial strain that produces succinic acid • Expression of proteins in E. coli for protein structure determination.• Improved throughput for protein production for proteomics using disposable two liter plastic bottles. • Produced selenomethionine labeled proteins for structure determination.• Developed method for enzymatically converting starch waste streams to glucose. • Used HPLC for analysis and quantitation of fermentation products. Show less -
Biology Laboratory InstructorJoliet Junior College Sep 1998 - Jun 1999Joliet, IlInstructor for lecture and lab class including cell biology, chemistry, genetics, photosynthesis, metabolism. -
Research And Industrial Products ManagerLos Alamos Diagnostics Oct 1986 - Mar 1989Los Alamos, Nm• Generated contacts and collaborated with universities and industry to develop methods for rapid detection of microorganisms, cell viability, and other applications of luminescent reactions. • Produce literature and generated strategies to promote reagents and instrumentation.
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Applications MicrobiologistPackard Instruments (Now Part Of Perkinelmer, Inc.) Aug 1978 - Oct 1986Downers Grove, Il• Tested and documented applications, reagents, and equipment based on luminescent reactions. • Presented luminescent methods at professional conferences and customer demos.• Defined reagent production methods and QA procedures using GMP and GLP.
Cynthia Millard Skills
Cynthia Millard Education Details
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Biology -
Business, Management, Marketing, And Related Support Services -
Microbiology
Frequently Asked Questions about Cynthia Millard
What is Cynthia Millard's role at the current company?
Cynthia Millard's current role is Research Scientist.
What is Cynthia Millard's email address?
Cynthia Millard's email address is cs****@****hoo.com
What is Cynthia Millard's direct phone number?
Cynthia Millard's direct phone number is (630) 252*****
What schools did Cynthia Millard attend?
Cynthia Millard attended Illinois Institute Of Technology, Lewis University, University Of Illinois At Urbana-Champaign.
What are some of Cynthia Millard's interests?
Cynthia Millard has interest in Animals, Art, Reading, Science Fiction, Health, The Environment, Children, Biotechnology, Sewing, Environment.
What skills is Cynthia Millard known for?
Cynthia Millard has skills like Protein Purification, Protein Expression, Hplc, Cell Culture, Protein Chemistry, Microbiology, Dna, Molecular Biology, Biotechnology, Life Sciences, Purification, Western Blotting.
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