X. Cyril Chen (He/Him) Email and Phone Number
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Accomplished biomedical engineer with Research and Development expertise and achievements in microfluidics, optics and photo-physics. 15 years’ experience in design & engineering hardware automation module and complete systems for complex biological workflow and integration in both industrial and academic settings. Demonstrated success in dynamic start-up environment showcased by being an essential contributor for successful multi-million-dollar acquisition. Areas of expertise include: • Next Generation Sequencing (NGS) instrument design, build, maintenance and revisions from breadboard to pre-commercial prototype• Design & optimization of microfluidic systems for precise delivery of small volume of costly reagents• Full prototype system build including NGS sequencers, laser scanning multiphoton microscopes, ultrafast transient absorption spectrometers with femtosecond lasers• Fluorescence applications for DNA and protein detection and characterization • Multiple disciplines of light microscopy including epifluorescence, confocal, multiphoton, second-harmonic imaging, STED of super-resolution, and TIRF.• Cell culturing, fixation and immunostaining for cell focal adhesions and F-actin filaments.• Serial immunohistochemical (IHC) staining for multiple antigens on FFPE tissue slides• Solidworks for mechanical designs.• 3D printing for fast hardware prototyping and lab equipment improvements.• Motion (stages) and temperature (thermal cycling and isothermal) control.• LabView programming for instrument control, data acquisition and analysis.• Image analysis using ImageJ and LabView.• Spectroscopic skills for detection of gas and liquid molecules, including UV-VIS, FTIR, Laser spectrometers like ICOS and LIDAR, and platereader for fluorescence and absorption• Strong photonics background including light sources, photodetectors, optical filters, polarization components, and nonlinear optics.• Extensive experience with characterization and application of pulsed lasers.
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Principal Systems EngineerFactorial Biotechnologies Sep 2022 - PresentSan Carlos, Ca, UsLead workflow automation of single cell genomics. -
Principal Product EngineerBerkeley Lights, Inc. Nov 2021 - Jul 2022Emeryville, Ca, UsResponsibilities at Berkeley Lights Inc. focused on improving product quality of the flagship Beacon optofluidic system, an automated on-chip cell culturing, imaging, manipulating machine with versatile single cell capabilities. Led investigation and resolution of system and assay for customer concerns that involved multiple departments/disciplines. Designed and performed tests for root cause and failure mode analysis. Provided guidance for design practices and controls to improve system reliability. Reviewed specifications for new product development. -
Staff EngineerElement Biosciences Oct 2020 - Nov 2021San Diego, California, UsJoined a system engineering team to develop the AVITITM sequencing platform based on the Element Avidity Sequencing chemistry. The sequencing platform is a high throughput imaging system with temperature and fluid controls for automated NGS sequencing workflows. The role focused on system integration and validation. Also led developing tools and methods to test sequencer performance. -
Principal Biological EngineerAgilent Technologies May 2018 - May 2020Santa Clara, Ca, Us(Agilent Technologies acquired Lasergen, Inc. in 2018)Lead Engineer for development of sequencer instrument for the innovative NGS sequencing technology based on Lightning TerminatorTM chemistry. The R&D role involved working closely with molecular biologists, chemists and software developers and required a combination of engineering efforts in different disciplines and a working knowledge of biological and biochemical sciences• Improved performance metrics and turn-around-time by performing NGS experiments by modifying modules including motion, fluidics, temperature controllers.• Developed several off-sequencer systems and analyses as orthogonal tests to identify and implement performance improvement projects.• Collaborated with OEM engineering firms for transfer of development and manufacturing phase of the sequencers, by knowledge transfer, overseeing projects on several modules and reviewing verification testing protocols and results. • Interacted with several cross-functional and global teams for multiple ongoing projects identified by milestone-based OKRs (objective and key results) following several project management schemes including Agile, Scrum, Confluence, Jira and version control systems.• Lead and resolved several critical troubleshooting discussions including root cause analysis, repeatability, reproducibility, verification, validation, tracking and trending and performance-based issues.• Participated in advancing the program intellectual property value with several inventions.• Responsible for writing and presenting scientific summaries of several key advancements to diverse audience including engineering, biology, chemistry and management teams -
Staff ScientistLasergen Oct 2011 - May 2018Houston, Texas, UsLead Engineer for development of sequencer instrument for the innovative NGS sequencing technology based on Lightning TerminatorTM chemistry. The R&D role involved working closely with molecular biologists, chemists and software developers and required a combination of engineering efforts in different disciplines and a working knowledge of biological and biochemical sciences.• Designed and built prototype sequencers for early phase proof of principle research of LT chemistry and optimized hardware and protocols to advance the technology, as an essential contributor to the Lasergen acquisition by Agilent Technologies for $195 million in 2018. • Designed and executed custom, non-standard experimentation to investigate and resolve challenging issues including non-specific dye binding on flowcell surface, uneven reactivity, high failure rate of critical modules.• Implemented and supported several SOPs following Quality Standards using ISO 13485 principles.• Implemented preventative maintenance programs for sequencers and trained junior engineers in operation, maintenance and troubleshooting of modules• Evaluated single molecule capabilities of prototype systems by hardware redesign, proof of principle experiment design and result summary• Modified sequencer to enable automated serial immunohistochemical (IHC) staining and imaging for multiple antigens on FFPE tissue slides with photocleavable fluorescence probes. -
Postdoctoral ResearcherUniversity Of Wisconsin-Madison Sep 2010 - Oct 2011Madison, Wi, Us(Research group relocated to UW Madison in 2010)Worked in Dr. Paul Campagnola’s group to develop innovative nonlinear optical methods to study cancer cells and tissues for disease diagnosis and investigation of cancer metastasis mechanism.• Built a multiphoton microscope used for 3D fabrication of protein structures as models of extracellular matrix (ECM) enabling study of cell haptotaxis and cancer metastasis.• Developed cell immunostaining protocol to stain focal adhesions and cytoskeleton to investigate cell-ECM interaction.• Developed a circular dichroism (CD) method for second-harmonic generation (SHG) microscopy and successfully utilized it to detect abnormal collagen structure in diseased osteogenesis imperfecta (OI) connective tissue. -
Postdoctoral ResearcherUniversity Of Connecticut Health Center Sep 2006 - Aug 2010Farmington, Connecticut, UsWorked in Dr. Paul Campagnola’s group to develop innovative nonlinear optical methods to study cancer cells and tissues for disease diagnosis and investigation of cancer metastasis mechanism. • Built a multiphoton microscope used for 3D fabrication of protein structures as models of extracellular matrix (ECM) enabling study of cell haptotaxis and cancer metastasis. • Developed cell immunostaining protocol to stain focal adhesions and cytoskeleton to investigate cell-ECM interaction. • Developed a circular dichroism (CD) method for second-harmonic generation (SHG) microscopy and successfully utilized it to detect abnormal collagen structure in diseased osteogenesis imperfecta (OI) connective tissue.
X. Cyril Chen (He/Him) Skills
X. Cyril Chen (He/Him) Education Details
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University Of Southern CaliforniaPhysical Chemistry -
University Of Science And Technology Of ChinaChemical Physics
Frequently Asked Questions about X. Cyril Chen (He/Him)
What company does X. Cyril Chen (He/Him) work for?
X. Cyril Chen (He/Him) works for Factorial Biotechnologies
What is X. Cyril Chen (He/Him)'s role at the current company?
X. Cyril Chen (He/Him)'s current role is Systems Engineer in Biosciences.
What is X. Cyril Chen (He/Him)'s email address?
X. Cyril Chen (He/Him)'s email address is cc****@****chc.edu
What schools did X. Cyril Chen (He/Him) attend?
X. Cyril Chen (He/Him) attended University Of Southern California, University Of Science And Technology Of China.
What skills is X. Cyril Chen (He/Him) known for?
X. Cyril Chen (He/Him) has skills like Cancer Research, Spectroscopy, Research And Development, Lasers, Troubleshooting, 3d Printing, Cell Culture, Engineering, Immunostaining, Microfluidics, Labview, Cancer.
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