D Wayne Lang Email & Phone Number

2000-2008Oncology Target Discovery through INDTarget discovery by retroviral library screening, followed by protein validation using standard Cell Biology techniques. Protein expression in E. coli, sf21 and Mammalian cell lines. Si RNA transfections combined with rt-PCR and Pulsed BrdU Cell cycle FACs analysis were used to evaluate targets. ELISA assays, In Cell Western assays ,Western blots, proliferation assays and Cell Cycle selected FACS screening assays were used in support of SAR on selected targets. Validated Oncology targets were partnered to collaborators, and additional targets kept for in-house use, which led to an IND/clinical trials with the Aurora Kinase inhibitor R763. 2008-2016Discovery Biology,moving from Small molecule to Antibody developmentCell and Molecular Biology for Metabolic and Immune Oncology IndicationsUse of standard cloning techniques and ligase independent methods for manipulation of transcription enhancers, promoters and gene family members in order to create protein expression vectors. Transient transfection for assays, and selection of cell lines for Immuno-Oncology and Muscle metabolism target work (assays and protein production). Transfections of mammalian cell lines, drug selection, protein expression and concentration in the Expi-293 cell line for small scale preclinical antibody experiments. Assay development as needed: Biochemical assay development for small molecule inhibitors of various members of the TGFb receptor family, including ELISAs, FRET assays, Lanthascreen binding assay, and the AlphaScreen SureFire assay. Small Molecule inhibitor projects also included RT-PCR and western blots and small animal MRI image acquisition and analysis, Subcloning , PCR and nucleic acid purification were performed when required.Alanine Scan for Epitope Mapping of the dimerization regions of the PVR1/Tigit interfaces. (Q5 mutagenesis based on crystal structures)

Listed skills include Molecular And Cellular Biology, Protein Expression, Site Directed Mutagenesis, Rt Pcr/Taqman, and 3 others.

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  Colby College, Sfsu

  Cell Biology