Daisy Bhatia Email & Phone Number

Utrecht, Netherlands

Melbourne, Victoria, Australia

Project title: A novel immunotherapy to enhance the therapeutic efficacy of radiotherapy against Glioblastoma: Adoptive Transfer of genetically engineered monocytesRelevant techniques learnt: molecular cloning, primer design, assay development and troubleshooting; mammalian cell culture, cell lines and primary cells (mESCs, mouse bone-marrow derived monocytes, PBMCs, bEnd3, GL261, L929 cells, etc) gene delivery by cell transfection (electroporation); mouse handling, monitoring and treatment (GL261 tumor model); ex-vivo assays, flow cytometry, RT-qPCR.

Lausanne, Vaud, Switzerland

Project title: study and optimization of antibodies against pathological TDP-43.Relevant techniques learnt: Development of functional assay to detect relevant proteinopathy-linked mRNA maturation dysfunction in human cell lines by RT-PCR and RT-qPCR; antibody purification with protein A resin, protein analysis by SDS-PAGE and western blot; cell immunostaining and microscopic analysis; mammalian cell culture (SH-SY5Y, HEK293 and CHO cells); primer design, gene delivery by cell transfection and transduction (using adeno-associated virus, AAV).

Lausanne, Vaud, Switzerland

Project title: Development of IL-21 secreting CAR-NK cells: molecular cloning and in vitro therapy testing assaysRelevant techniques learnt: DNA cloning pipeline, transfection and transduction of CAR constructs, cell culture techniques (HEK293 and NK cells), cell-based assays (coculture experiments, FACS analysis, etc).

Lausanne, Vaud, Switzerland

Project title : Development of in vitro protocol for the generation of exhausted CD8+ T cells using repeated peptide stimulation, to study the effect of different immunosuppressive factors and improve the efficacy of T cell-based therapiesRelevant techniques learnt: mouse T cell isolation, mammalian cell culture techniques (CD8+ T cells), FACS analysis.

Lausanne, Vaud, Switzerland

Project title : Generation of cGAS mutants to study cGAS regulation during the cell cycle.Relevant techniques learnt: DNA cloning pipeline and design strategies, genotyping, quantitative real-time PCR, Sequencing, SDS Page and Western Blot, mammalian cell culture basics. Snapgene and Benchling softwares.

Lausanne, Vaud, Switzerland

Teaching assistant for the core course Analysis IV for second year bachelor students in Life Sciences Engineering.Daily activities: mentor and supervise second year Bachelor's students, manage exercisesessions in person and remotely on zoom, collaborate with other teaching assistants to adaptthe exercise sessions

Master'S Degree, Life Sciences Engineering

Bachelor'S Degree, Life Sciences Engineering