Dan Altier Email & Phone Number

Johnston, IA, US

Johnston, IA

Johnston, IA

• Insecticidal protein discovery and analysis.Senior Research Associate (SRA) III (2011 – 2018), SRA II (2001 – 2011)
• FPLC purified insecticidal proteins from plant, bacteria, and fungal sources leading to numerous novel protein discoveries for deployment in transgenic plants.
• Used E. coli expression systems and protein purification (analytical to gram scale) for construct characterization and high purity protein production for use in protein characterization, mode of action studies, and.
• Prepared trypsin digest samples of PAGE gel bands and chromatography fractions for MS/MS analysis to evaluate lead purification and enable rapid purification progress minimizing time spent on previously identified.
• Performed PAGE and Western blotting to evaluate transgenic plants for target protein expression enabling rapid transgenic event sorting and correlation of protein expression with plant efficacy data.
• Ran simulated gastric fluid digestion and SDS PAGE analysis to evaluate proteins for digestibility and identify potential allergenicity problems.

Johnston, IA

• Plant disease resistance, antifungal protein discovery, and secondary metabolite analysis.Senior Research Associate (SRA) II (2001 – 2011), SRA I (2000 – 2001), RA II (1995 – 2000).
• Executed HPLC-TOF-MS (time of flight mass spectroscopy) for the rapid purification and identification of antifungal proteins leading to novel protein discoveries for deployment in transgenic plants.
• Carried out HPLC analysis to quantify plant metabolites in response to chemical or mechanical treatments or transgenic protein expression.
• Accomplished HPLC plant metabolite profiling and characterization from recombinant inbred plant populations to identify metabolites correlated with plant phenotypical data.
• Developed colorimetric, fluorescent, and chemoluminescent assays for transgenic proteins enabling rapid transgenic event evaluation.

Master Of Science (Ms), Biochemistry

Thesis title – “Purification and Spectroscopic Characterization of a Two Component Benzoate 1, 2- Dioxygenase Form Pseudomonas putida.”

Bachelor'S Degree, Chemistry And Biology