Dan Gonzalez Email & Phone Number

Ronkonkoma, New York, United States

Growing embryonic stem (ES) cells for their injection into mouse blastocysts as part of the production process towards genetically engineered mouse models.Maintaining biological cabinets, incubators and other lab equipment sterile by practicing proper aseptic technique and routine cleaning.Feeder cell preparation and irradiation.ES cell electroporation.Neuro2a cell transfection.Coordinating with other departments for injections, DNA extractions, ES cell expansion/growth and electroporation.Maintaining records of ES cell clone storage, electroporation, clone expansions, dna extractions.Maintaining detailed notes on cell quality, morphology, and confluency during experiments for quality control and troubleshooting

Ronkonkoma

Providing assistance to senior scientists in tasks such as DNA sequencing, molecular cloning procedures including PCR, DNA prep, Gel - electrophoresis, the harvesting and clarification of bacterial cultures,enzyme digestion and analysis, and DNA transformation.Prepping laboratory equipment and materials, making buffers, media and reagents. Accounting for, purchasing and procurement of lab supplies and reagents.Autoclaving and sterilizing lab instruments and glassware as needed.Maintaining lab and work-spaces up to OSHA standards. Keeping and disposing of materials according to the GHS, SDS, and MSDS guidelines.