Daniel "Chris" Mclendon Email & Phone Number

Alachua, Fl, Us

· Assist with development and validation of in vivo transcription of an Artificially Expanded Genetic Information System (AEGIS) employing modified nucleic acids· Assist with characterization and validation of quantitative assay for detection of AEGIS oligonucleotides in vivo (prokaryotic) by PCR and HPLC· Assist with optimization and methods development for solid phase phosphoramidite based oligonucleotide synthesis with modified bases (AEGIS). Oligonucleotide purification by IE-HPLC/RP-HPLC or PAGE.· Provide custom manufactured oligonucleotides, consulting with customers and collaborating scientists to design specific oligos optimized for downstream applications (Next generation sequencing, diagnostics, SELEX aptamers, gene assemblies)

· Customer engagement, by TEAMS, phone, email discussing workflow, trouble shooting, consultation and providing solutions with IDT’s Next Generation Sequencing product line. · Providing customer consultation regarding NGS design, library prep, hybridization capture and amplicon sequencing.· Prospecting new leads, determining BANT qualification, and preparing information for outgoing sales calls or emails to secure new customers.· Funnel management, prospecting and customer engagement documentation management using CRM and related software (SalesLoft, PowerBI, Salesforce)

Alachua, Florida, Us

• Manage and maintain DNA synthesis laboratory• Maintain oligonucleotide synthesis orders (procurement, delivery, retains and QC) • Solid Phase (phosphoramidite) DNA synthesis chemistry specializing in modified internal and end terminal bases (eg. biotinlyation/ fluorescence labeling / LNAA’s etc.) including: amidite drying and preparation, anhydrous technique for resuspension, oligo synthesis, deprotection, crude analysis, HPLC/PAGE purification, desalting, QC analysis and delivery• Operation and preventive maintenance of DNA synthesizers instrumentation ABI3900, ABIO 394 and Mermade12• Maintain regulatory records and inventory of synthesis supplies and reagents• Research novel synthesis methods using modified bases and alternative capping and oxidation reagents

Alachua, Fl, Us

Oligonucleotide synthesis using CustomArray synthesizerDetermination of solubility of organic compounds in liquid propaneResearch investigation of pre-biotic chemistry on the lakes of TitanStructural analysis of hydrocarbons by Nuclear Magnetic Resonance (NMR)Gene assembly with Kinase dependent annealing, extension and ligationDevelopment of point of care isothermal nucleic acid detection assays targeting infectious agents including real time helicase dependent assays (HDA) using Roche Light Cycler Development of reverse transcriptase RT- HDA assays targeting HIV-RNA (ART resistance mutants)Development of multiplex helicase dependent assays (HDA) employing artificial SAMRS (Self Avoiding Molecular Recognition System) nucleotidesOptimization of SELEX (systematic evolution of ligands by exponential enrichment) technology(optimizing PCR amplification and digestion methods for accurate nucleotide conversion of natural vs. Artificially Expanded Genetic Information System (AEGIS) nucleotides). PAGE purification of natural, SAMRS and AEGIS oligonucleotides

Gainesville, Fl, Us

Develop and validate methods for qualitative immunoassays used for the species determination of meat in agricultural and commercial food products.Develop methods for detection of CNS tissue in heat processed animal feeds.Develop thermal reference meals for characterization of immunoassays.Calibration and maintenance of general laboratory equipmentPrepare graphs and summaries of data for posters, abstracts, and presentations.Write and review product literature and instructional material.

Gainesville, Fl, Us

Develop and validate methods for qualitative immunoassays used for the species determination of meat in agricultural and commercial food products.Formulations: optimization of ELISA buffers and reagents; sample extraction, blocking, assay diluent, conjugate diluent, initial characterization of IgG's.Column Chromatography - separation and purification of IgG's and antigen Preps.Column derivitization using various protein-ligand coupling methods -NHS, Periodate, SH. Coupling of CL4B-NHS columns or gluteraldehyde cross linked gel matrices for Immunoadsorption and/or affinity purification of IgG's.Coordinating the procurement, characterization and quality assurance of incoming raw materials and reagents used in immunoassays prior to release to production departmentProject development and management.Documents coordination – SOP’s, spec sheets, MSDS sheets, Batch Records.Provide technical assistance and troubleshooting for customers over the telephone.Provide on-site training/demonstration and technical support; develop training materials and electronic/ visual aids (Powerpoints/ PDF’s/ Technical Literature).Employee Training & Supervision.

Bethesda, Md, Us

Develop and validate methods for characterization of in vitro enzymatic activity of E3 ubiquitin ligases including: ubiquitination assays; gene silencing (siRNA); Enzyme purification; Protein Stability studies including: Radiolabeled pulse-chase studies (35S-Methionine); Immunoprecipitation and Western Blotting.Develop and validate methods for analysis of proteins and peptides by Surface Enhanced Laser Desorption Ionization-Time of Flight (SELDI-TOF) Mass Spectrometry.Molecular Cloning and Protein Expression including: purification, enzymatic digestion, ligation of plasmid DNA; site directed mutagenesis.DNA sequencing using capillary based fluorescent technology (ABI Prism).Mammalian/Bacterial Cell Culture; creating and maintaining pooled stables and clonal cell lines.Cytotoxicity Assays (MTT) using insecticides (mitochondrial complex-1 inhibitors) and other pro-apoptotic compounds.

Rochester, Minnesota, Us

Develop and validate methods for in vitro enzymatic activity from detergent purified membrane preparations including: Immunoprecipitation and Western Blotting; Enzyme Linked Immunosorbent Assays (ELISA's).Develop and validate methods for expression of fusion protein in bacterial cells (E.Coli):, cloning inducible expression vectors, PCR; restriction enzyme digestion, gel purification, and ligation of DNA.Develop and validate methods for purification of novel proteins including: glycerol or sucrose gradient fractionation, size exclusion chromatography, nickel purification of poly-histidine tagged proteins.Develop and validate methods for antibody screening, purification, conjugation and testing for use in ELISA, Western Blotting and Immunohistochemistry.Train & Supervise medical/graduate students and interns in Laboratory/Research methods.Column affinity purification of peptides, SDS-PAGE (coomassie and silver staining), Western Blotting, 2D-gel electrophoresisIn vitro fluorogenic assays for enzymatic activity, ELISA (competitive and sandwich), Biotin crosslinking (Streptavidin capture), Purification of DNA by maxi/mini prep; Enzymatic digestion of DNA, Ethanol precipitation of DNA, Transformation of plasmid DNA into bacterial cells, transfection of DNA into mammalian cells, Mammalian/bacterial cell culture, Creating and maintaining pooled stables, Separation and subcellular fractionation by differential centrifugation, RP-HPLC (UV/Vis)

Laboratory/Office management.Design, perform & statistically analyze data from Neuropharmacological experiments.Prepare written summaries & graphical representation of experimental data for publication in Scientific/Medical Journals.Train & Supervise medical/graduate students and interns in Laboratory/Research methods.Clinical Research Coordination: assisting with budget considerations and modifications & amendments to experimental protocols; direct patient contact; connecting patient to biological-monitoring systems, conducting physiological/behavioral experiments, collecting physiological data; processing, storage and subsequent analysis of blood samples from patients.

Laboratory/Office Management.Operation & maintenance of HPLC systems.Integration & Data analysis.HPLC analysis of hormone levels in human plasma.Design & conduct in vitro experiments.

Omaha, Nebraska, Us

Primary Cell Culture including: isolation, identification, culture & storage of several different cell type (aortal/cerebrovascular endothelial cells, monocytes).Operation & maintenance of most types of HPLC & cell culture equipment.Laboratory/Office Management.

Biotechnology

Receptor Pharmacology

Bachelor Of Arts, Environmental Studies