T cell Characterization-Leading team focused on deep phenotypic and functional characterization of engineered T cell drug product and donor material-Characterizing allogeneic donor material, intermediates, unit ops, and product attributes and how these effect process performance, product profile, and functionality -Developing analytical tools and models to study T cell differentiation, cytotoxicity, and function

Process Sciences - T cell engineering Intellia Therapeutics is a leading genome editing company focused on developing curative therapeutics using the CRISPR/Cas9 system.Duties Include:-Lead scientist for the scale-up, process development, and optimization of NTLA-6001, an allogeneic CRISPR-Cas9 gene edited CAR T cell drug product targeting CD30 for the treatment of lymphoma-Process development technical lead and SME for NTLA-5001, an autologous CRISPR-Cas9 gene edited tgTCR T cell drug product targeting WT1 for the treatment of acute myeloid leukemia

T cell Product CharacterizationJuno Therapeutics is a clinical-stage company developing novel cellular immunotherapies based on two distinct and complementary platforms – Chimeric Antigen Receptor (CAR) and T Cell Receptor (TCR) technologies. Juno’s goal is to revolutionize medicine by re-engaging the body’s immune system to treat cancer.Duties Include:-Supporting the development of JCAR017, an anti-CD19 CAR-T cell product, using in-process analytics to help understand cellular dynamics during manufacture.-Developing analytical tools to better understand the behavior and capabilities of genetically modified T cells against hematological malignancies. -Designing experimental methods that contribute to the development of safe and efficacious CAR-T cells.

CAR-T Cellular Process Development & Manufacturingbluebird bio is developing potentially transformative, one-time gene therapies for severe genetic and orphan diseases. At the heart of bluebird bio’s product creation efforts is its broadly applicable gene therapy platform for the development of new treatments for diseases with few or no clinical options. bluebird bio has two clinical stage product candidates in development, one for childhood cerebral adrenoleukodystrophy (CCALD) and one for beta-thalassemia major/sickle cell disease, and an early stage CAR-T cell program in partnership with Celgene Corporation. Duties included:-Contributing to the clinical scale, closed system development of two anti-BCMA CAR T cell product candidates; bb2121 and bb21217.-Executing procedures for the isolation, transduction, expansion and cryopreservation of gene modified autologous T cells for the treatment of relapsed/refractory multiple myeloma.-Responsible for coordinating and executing large scale expansions of gene modified T cells to support in house in vivo studies.-Conducting testing methods for the characterization of in process and post expansion T cell subpopulations via Flow Cytometry, qPCR, and ELISA.

Channing LaboratoryThe Channing Division of Network Medicine is a research division within the Department of Medicine at Brigham and Women’s Hospital. The Mission of the Channing Division of Network Medicine is: To use an integrated, network-based, systems biology-driven approach to define the etiology of complex diseases; to reclassify complex diseases based on systems pathobiological mechanisms; and to develop new treatments and preventive strategies based on these new disease classifications using systems pharmacology approaches.Duties included:-Supervising technical procedures in the bio-repository, such as; human specimen processing, RNA and DNA extractions, plating, cell stimulation, and bioassays. Responsible for the training and safety of all laboratory personnel.-Overseeing the entire laboratory cryostorage system and software. -Conducting equipment maintenance, repairs, and standardizations.-Designed and piloted over a half dozen genetic studies beginning with sample collection and leading to various downstream applications.

Brigham and Women's Hospital DNA Sequencing CoreThe purpose of the Brigham and Women's Hospital DNA Sequencing Core is to provide a high-throughput, accurate, high quality DNA sequencing service to the Longwood Medical research community.Duties Included:-Performing PCR reactions in preparation for sequencing-Maintaining and operating the Hitachi 3730XL DNA Analyzer-Organizing data for customer analysis