• Independently planned and executed cytometry assays with biotherapeutic molecules utilizing flow- and imaging flow-cytometry instruments, the FACSymphony A5 and the Amnis ImageStream, respectively• Developed, adapted and optimized flow- and imaging flow-cytometry assays • Applied advanced knowledge of multi-parameter flow cytometry to effectively develop and optimize complex cell-based bioanalytical and functional assays• Critically analyzed experimental data and communicated the results across multiple teams with diverse scientific and technical backgrounds• Collaborated within and without the group; fostered partnerships in a matrix environment; worked closely with cross-functional teams to plan and execute deliverables and prioritize activities according to project goals• Adhered to scientific rigor and quality management system standards, including electronic record keeping using ELN, data peer-review, quality control and reporting• Complied with all safety training and good laboratory practices

• Contributed key design features to a microfluidic platform for modeling tumor-immune interactions• Collaborated in multidisciplinary teams to complete complex project objectives on tight timelines• Developed methods and protocols for novel platforms• Worked on several microfluidic cell processing projects, including electroporation, viral transduction, and acoustic separation• Coordinated the planning, execution, and analysis of experiments with electrical and mechanical engineers• Isolated, cultured, and characterized immune cells from primary human tissues• Devised multi-color panels for both flow cytometry and confocal microscopy, in order to identify and characterize cell populations• Presented data and key concepts to audiences with widely varied backgrounds

• Characterized and profiled tumor infiltrating lymphocytes on a population level by flow cytometry, and on the single cell level using Enumeral’s proprietary platform• Successfully led a screening campaign that yielded a patent application for matched heavy and light chain antibody sequences against a specific immuno-modulatory receptor• Developed cell-based functional assays to compare novel drug candidates with competitors• Devised and performed screens of primary B cells from autoimmune patients for specific anti-self antibodies• Devised and performed screens of individual primary human B cells for specific anti-viral antibodies• Improved overall yield and reliability of Enumeral’s platform through process optimization, developing it from its academic origins to a much more robust, commercially viable technique• Used varied imaging techniques such as multi-color flow cytometry, high-content imaging, fluorescence microscopy, multiplexed protein microarrays, and fluorescence-based virus neutralization assays• Managed and mentored a research assistant

• Executed the experiments required for a successful Investigational New Drug (IND) application with the FDA.• Performed functional characterization of novel monoclonal antibodies that modulate immune function.• Analyzed the method of action (MOA) of the antibody in vivo using murine tumor models.• Analyzed the MOA of the antibodies using ex vivo and in vitro cellular immunology studies including flow cytometry, ELISPOT, cell proliferation studies, mixed lymphocyte reaction (MLR), etc.• Developed and optimized cell-based- and flow cytometry-based assays, ELISAs, and Biacore™ assays.• Mammalian cell culture, animal handling, and human tissue handling in a BL2 environment.• Regularly presented results to Research and Pre-Clinical departments.• Managed day-to-day operations and directed contract worker on anti-drug hybridoma project. • Two publications in preparation at time of staff reduction.

• Measured in vivo antigen-specific immune response to vaccine candidates with both cell-based and biochemical-based assays (with FACS, ELISA, ELISPOT, viral plaque assays, etc.).• Developed and optimized cell- and flow cytometry-based assays.• Conducted clinical trial experiments using Good Laboratory Practices.• Collaborated in a wide variety of multidisciplinary, team-based projects.• Virology, mammalian cell culture, and animal handling in a BL2 environment. • Laboratory Safety Committee member representing the Immunology group.• Trained by BD Biosciences to operate and maintain the FACSCanto cytometer.• Regularly presented results to colleagues.