Daniel Hogan Email & Phone Number

San Mateo, Ca, Us

S San Fran, California, Us

Redwood City, California, Us

• Identification of predictive biomarkers for retroviral gene therapy (Toca511) targeting glioblastoma multiforme.• Development of minimally invasive diagnostics for glioblastoma multiforme.

San Diego, Ca, Us

• Developed new design principles for oligonucleotide therapeutics targeting microRNAs (anti-miRs) that were incorporated into Regulus’ first developmental candidate RG101.• Identified mRNAs whose increased expression correlated with undesirable effects associated with some anti-miRs in model organisms using molecular profiling data, providing an integral component of the drug discovery cascade.• Defined the mechanism of action of anti-miRs in vivo. • Designed cell culture assays to eliminate pro-inflammatory and cytotoxic anti-miRs.• Characterized intra-tissue and intracellular trafficking of anti-miRs in mouse liver using biochemical separation techniques.• Established workflow to quantify anti-miR levels in specific cell types and subcellular compartments in mouse livers.• Presented research findings to Board of Directors and Scientific Advisory Board on several occasions.

Palo Alto, Ca, Us

• Analyzed DNA microarray data from hundreds of human adipocyte samples in order to characterize site-specific developmental and physiological programs.• Developed a ribosome footprinting/RNA sequencing method to systematically map the location of ribosome-associated chaperone interactions with nascent polypeptides.

Palo Alto, Ca, Us

• Systematically identified the RNA targets of 40 RNA-binding proteins in yeast by affinity immunopurification and DNA microarray hybridization.• Discovered an extensive combinatorial system for post-transcriptional regulation involving dozens or even hundreds of RNA-binding proteins.• Developed a method to systematically identify the mRNAs recruited to Argonuate proteins by specific microRNAs.• Applied a novel systematic translation profiling method to dissect the mechanism of action of microRNA-mediated regulation of gene expression.• Co-developed a method to systematically identify the nascent polypeptides associated with specific molecular chaperones.

• Used subtractive cDNA screen to Identify mRNAs differentially expressed during macronuclear development in Stylonchia lemnae.• Identified PIWI protein as being differentially expressed during macronuclear development and proposed PIWI-associated small RNAs from parental macronucleus guide DNA recombination events.• Developed RNAi method for stichotrichous ciliates by feeding them E. coli engineered to produce dsRNA of the gene of interest.

Boulder, Colorado, Us

• Developed a method to eliminate contaminating macronuclear DNA from micronuclear DNA preparations in stichotrichous ciliates.• Demonstrated the order of IES insertion and gene scrambling of the actin I gene in stichotrichous ciliates, which we published in PNAS.

Phd, Molecular Biology, Biochemistry, Genomics

Bachelor Of Science (Bs), Mcd Biology, Biochemistry With A Minor In Math