Daniel Hogan Email and Phone Number

• Identification of predictive biomarkers for retroviral gene therapy (Toca511) targeting glioblastoma multiforme.• Development of minimally invasive diagnostics for glioblastoma multiforme.

• Developed new design principles for oligonucleotide therapeutics targeting microRNAs (anti-miRs) that were incorporated into Regulus’ first developmental candidate RG101.• Identified mRNAs whose increased expression correlated with undesirable effects associated with some anti-miRs in model organisms using molecular profiling data, providing an integral component of the drug discovery cascade.• Defined the mechanism of action of anti-miRs in vivo. • Designed cell culture assays to eliminate pro-inflammatory and cytotoxic anti-miRs.• Characterized intra-tissue and intracellular trafficking of anti-miRs in mouse liver using biochemical separation techniques.• Established workflow to quantify anti-miR levels in specific cell types and subcellular compartments in mouse livers.• Presented research findings to Board of Directors and Scientific Advisory Board on several occasions.

• Analyzed DNA microarray data from hundreds of human adipocyte samples in order to characterize site-specific developmental and physiological programs.• Developed a ribosome footprinting/RNA sequencing method to systematically map the location of ribosome-associated chaperone interactions with nascent polypeptides.

• Systematically identified the RNA targets of 40 RNA-binding proteins in yeast by affinity immunopurification and DNA microarray hybridization.• Discovered an extensive combinatorial system for post-transcriptional regulation involving dozens or even hundreds of RNA-binding proteins.• Developed a method to systematically identify the mRNAs recruited to Argonuate proteins by specific microRNAs.• Applied a novel systematic translation profiling method to dissect the mechanism of action of microRNA-mediated regulation of gene expression.• Co-developed a method to systematically identify the nascent polypeptides associated with specific molecular chaperones.

• Used subtractive cDNA screen to Identify mRNAs differentially expressed during macronuclear development in Stylonchia lemnae.• Identified PIWI protein as being differentially expressed during macronuclear development and proposed PIWI-associated small RNAs from parental macronucleus guide DNA recombination events.• Developed RNAi method for stichotrichous ciliates by feeding them E. coli engineered to produce dsRNA of the gene of interest.

• Developed a method to eliminate contaminating macronuclear DNA from micronuclear DNA preparations in stichotrichous ciliates.• Demonstrated the order of IES insertion and gene scrambling of the actin I gene in stichotrichous ciliates, which we published in PNAS.