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Research associate specializing in insect cell culture and recombinant protein production utilizing the BEV system. Focus in selection, testing, and production of high yield viruses. Additional experience in molecular biology and cloning techniques
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Associate Director - Mrna Process DevelopmentTome Biosciences Feb 2023 - Aug 2023
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Associate Director, Process DevelopmentGeneration Bio Nov 2021 - Feb 2023Cambridge, Massachusetts, Us -
Sr. Scientist - Upstream Process DevelopmentGeneration Bio Dec 2019 - Nov 2021Cambridge, Massachusetts, Us
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Scientist - Research Production/Upstream Process DevelopmentGeneration Bio Jun 2018 - Dec 2019Cambridge, Massachusetts, Us
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Research Associate - Upstream Process DevelopmentGeneration Bio Oct 2017 - Jun 2018Cambridge, Massachusetts, Us
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Strain Engineer (6-Month Contract)Ginkgo Bioworks, Inc. Feb 2017 - Jul 2017Boston, Ma, UsWorking in a molecular biology driven role with organism engineers and program teams to modify bacterial pathways and increase yields of products of interest- Constructed plasmids and recombinant bacterial strains to screen for product yields- Created modified E. coli genomes via Crispr/Cas9 system gene knock-outs- Assisted a late stage program to deliver final materials to the customer, and an early stage program to begin development of initial testing and proof of concept experiments- Performed basic microbiology techniques for E. coli and Streptomyces -
Research Associate Ii Molecular BiologyProtein Sciences Corporation Apr 2015 - Feb 2017Meriden, Ct, UsProduct Development/Product Realization group focused on molecular sub-cloning, qRT-PCR based assays, and increasing recombinant protein yields through manipulation of transfer plasmids.-Seamless cloning viral genes into proprietary transfer vectors. Manipulate and evaluate new transfer vectors.-mRNA extractions, design and execution of qRT-PCR primers/experiments to evaluate new constructs and illustrate presence or absence of adventitious viruses.-Optimize viral scale-up and transfection processes using traditional and novel baculovirology techniquesOther responsibilities include: write and review reports, summarize and present data, support and provide data/insight to upstream and downstream development groups, onboard new employees. -
Research Associate Ii Cell CultureProtein Sciences Corporation Jan 2011 - Mar 2015Meriden, Ct, UsProduct Development Up-Stream Development group, working specifically on developing processes for optimal production of various recombinant proteins for internal and external customers.-Insect cell culture and virus propagation in the baculovirus expression vector system (BEVS). Responsible for producing and identifying high yield clones for viruses expressing a variety of recombinant proteins .-Evaluate viruses by various cell and protein based assays. Including: PCR, SDS-PAGE, Western blot, morphology of infected cells (microscopic), titer (by plaque assay or InDevR VirocytTM2100 Virus Counter®), and infection kinetics.-Optimize fermentation conditions for recombinant protein in 3L and 15L Applikon bioreactors-Operate 5L, 15L and 45L CelliGen BLU (Eppendorf) single use bioreactors in small scale cGMP facility. Fully trained and experienced in cGMP procedures and conditions.-Respond to customer questions and needs. Adhere to specific timelines and schedules while managing work for multiple concurrent projects.-Other responsibilities include: design and execute experiments, collect and present data, and respond to customer questions and needs.
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Lab CoordinatorProtein Sciences Corporation Oct 2010 - Jan 2011Meriden, Ct, Us-Cleaned and sterilized all quality control (QC) and process development (PD) glassware and supplies, performed maintenance and cleaning on 3L and 15L Applikon bioreactors-Prepared all general use buffers for PD groups-Maintained lab cleanliness and organization.
Daniel Macri Skills
Daniel Macri Education Details
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Quinnipiac UniversityCell And Molecular Biology -
Marist CollegeChemistry
Frequently Asked Questions about Daniel Macri
What is Daniel Macri's role at the current company?
Daniel Macri's current role is Associate Director - mRNA Process Development at Tome Biosciences.
What is Daniel Macri's email address?
Daniel Macri's email address is dm****@****ces.com
What is Daniel Macri's direct phone number?
Daniel Macri's direct phone number is +120368*****
What schools did Daniel Macri attend?
Daniel Macri attended Quinnipiac University, Marist College.
What skills is Daniel Macri known for?
Daniel Macri has skills like Western Blotting, Genetics, Recombinant Protein Production, Pcr, Biotechnology, Cell Culture, Qrt Pcr, Seamless Cloning/gibson Assembly Method, Sds Page, Aseptic Technique, Plaque Purification, Colony Pcr.
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