Daniel Mcpartlin, Phd Email & Phone Number

Ireland

Dublin

Cruiserath, Dublin

Dublin

• Project Title: Development of recombinant antibody fragments for toxin and microbial contaminant detection and investigations of microcystin and azaspiracid toxicity.Supervisor: Prof. Richard O'Kennedy
• Successfully developed novel antibodies to detect azaspiracid (AZA), microcystin-LR (MC-LR) and B. cereus in seawater, freshwater and milk, respectively.
• Successfully discovered the toxicological effects of combinations of AZA and MC-LR on the human liver.
• Key technical skills: microbiology, bacterial protein expression, immunoassay development and validation, mammalian cell culture, ELISA, SDS-PAGE, Western Blot, PCR, chromatography (IMAC, SEC), data analysis. Software.
• Key transferable skills:o Problem-solving: In a PhD, you are constantly faced with new and varied tasks. This requires being able to think on your feet and to be adaptable in order to successfully achieve targets in a.
• Seven peer-reviewed publications, including three first-author articles.

School Of Biotechnology, Dublin City University

• Responsible for operating and maintaining Biacore 4000 and 3000 SPR systems.
• Successfully carried out Biacore analyses for international collaborators as well as members of the laboratory team. This work was featured in peer-reviewed publications.
• Liaised with GE Healthcare technicians and specialists to optimise the running of and outputs from the Biacore systems.

Dublin City University, Dublin, Ireland

During my time as Chair of the BRS, I helped to organise an outreach partnership with the Aisling Project in Ballymun. This partnership saw a number of PhD students and staff from the School of Biotechnology set up scientific workshops for the children of the Aisling Project. Also during my time as the Chair, the partnership between the BRS and the Aisling.

Dublin City University, Glasnevin, Dublin 9

• Responsible for the pilot-scale production of recombinant antibody fragments. Antibody production was scaled up from 500 mL shaker culture to a 10 L Eppendorf BioFlo 320 reactor. Antibodies were purified using.
• Carried out product quality checks to a high standard. This required analysis of samples by Äkta Pure analytical chromatography, SDS-PAGE and Western blotting analysis.
• Successfully produced and certified high purity antibodies that have been sold to laboratories around the world.

• Responsible for carrying out all bioburden water quality testing on site. This involved testing up to 50 samples per day of high purity, distilled or tap water using a number of analyses to extremely high standard of.
• Successful completion of my duties required intimate knowledge of regulatory specifications, strict adherence to current good manufacturing practices (cGMP), accurate documentation using LIMS and escalating issues as.
• Successfully identified a potentially harmful contamination in two operations systems. These issues were escalated with the operators and the contamination was dealt with immediately.

Doctor Of Philosophy - Phd, Biotechnology, 1.1

Bachelor Of Science (Bs), Biotechnology, First Class Honours

Activities and Societies: DCU gym, swimming, Biological Research Society.

Education record

Activities and Societies: St. Declan's gaelic football and hurling teams.