Stood up bioreactor fermentation from installation to optimized fermentation of filamentous fungiOptimized containment of exhaust and harvest of potent compound harvestsWrote a bioreactor fermentation SOPPerformed chemical natural product extraction for isolation and LCMS analysisAnalyzed fermentation samples using LCMS and biomass Wrote SOPs for techniques such as large scale culturing and CRISPR transformationsPerformed Gene ValidationDesigned transcription overexpression and knockout fungal strainsScreened for compound candidates using enzyme based assaysUsed for yeast forward genetics for target elucidationPresented company-wide talks for the fungal platformOptimized bioreactor fermentation with environmental and genetically engineered fungal strains

Implemented and Trouble-shot the CRISPR-Cas9 integration transformation workflowPerformed scale-up of environmental fungal strains to liter scaleWrote and edited SOPs for molecular techniquesDeveloped a high-throughput workflow for spore countingTrained Scientists and implemented an onboarding plan

Performed Fungal Transformations using automationScaled up culturing from microliter scale to 32 litersScreened compound candidates for anti-fungal activity including A. fumigatus, C. albicans, and S. cerevisiae

Designed primers for quality controlResearched and designed genetic editsCultured fungal strains in high through-put using automation equipmentCreated a whole-genome sequencing workflow to be used by my teamWorked with large datasets to optimize the design, quality control, and culturing of strains

Lead development of molecular assays specializing in qPCR and RT qPCR to identify viral and bacterial nucleic acidsWriting and editing methods and SOPs for GMP usePerformed trouble shooting for sequencing of drug productsReviewed data from GMP testing