Since graduating from the College of William and Mary in 2020, I have been developing protein-based and small molecule therapeutics in a cancer research lab at Virginia Commonwealth University. During my time in Dr. Fisher’s lab, I rapidly developed my skills as a researcher and lab manager, far beyond my initial job description, while simultaneously pursuing my master’s degree and thesis. Although my previous research experience was incredibly rewarding, I’m now looking to utilize my skills and knowledge for new opportunities where I can continue to make a difference.
York County Tourism Development
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Waterfront Operations SupervisorYork County Tourism Development Apr 2017 - PresentYorktown, Virginia, United States• Prepare and oversee all the events at the Yorktown Waterfront, ensuring that the farmer’s markets, concerts, and holiday celebrations proceed according to plan by coordinating with external vendors and other York County departments.• Serve as a representative for York County and maintain a positive public image with our community and visiting tourists.• Manage multiple janitorial, parking, and ambassador staff to maintain waterfront operations.• Responsible for venue showings and set up for the Yorktown Historic Freight Shed.
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Laboratory And Research Specialist IVirginia Commonwealth University Aug 2020 - May 2024Richmond, Virginia, United States• Primer design and plasmid construction for therapeutic fusion proteins and associated lentiviral vectors. • Construct expression by transformation and molecular cloning in E. coli, as well as lipofectamine transfection in mammalian cell culture.• Utilized lentiviral vectors to establish stable expression of generated constructs in Jurkat T-cells.• Evaluating changes in expression in response to fusion protein on immune cell proliferation and activation via RT-qPCR, ELISA, and western blotting. • Preparation of small molecule inhibitors for in vitro and in vivo assays.• Cell based assays such as colony formation and MTT/MTS. • Responsible for maintaining cell cultures for viral vector amplification, protein purification, and other experiments.• Process mice organ tissue and tumor samples for RNA or protein extraction.• Perform western blotting to investigate expression of target proteins.• Analyze cDNA produced from extracted RNA samples using RT-qPCR. • Maintain inventory of lab equipment, reagents, and cell lines in coordination with the postdocs and lab manager.• Plasmid isolations from bacterial culture for later use in cell culture transformation and animal studies.• Routinely perform quantification assays for protein and nucleic acid samples, typically BSA, Bradford method, and Nanodrop measurements.• Occasionally perform IHC staining on collected animal tissues.• Developed protocols, maintained various cell lines, and performed microinjection xenograft experiments on zebrafish embryos for our migration inhibitor study.• Trained new lab technicians and master’s students on lab techniques and lab operations.• Negotiating with vendors to acquire equipment and consumables to meet our lab’s need.• Contacting vendors for necessary services and repairs on lab equipment.• Perform a variety of other miscellaneous experiments, reagent preparation, and general lab duties in Paul Fisher’s lab. -
Vivarium AssistantWilliam & Mary Mar 2020 - Apr 2020Williamsburg, Virginia, United States• Maintained vivarium facilities by regularly cleaning enclosure rooms.• Disposed of waste appropriately and autoclaved vivarium equipment and animal enclosures.• Prepared clean enclosures for lab rats and mice.• Frequently checked on vivarium animals’ health, looked out for injuries or changes in behavior. -
Laboratory InternWilliam & Mary Jun 2019 - Aug 2019Williamsburg, Virginia, United States• Performed enrichments on outreach soil samples to obtain bacteriophages. • Routinely performed plaque purification on Mycobacterium achiense grown on agar plates to isolate bacteriophages. • Produced large volumes of high titer lysate by flooding M. achiense plates which had been plated with purified plaque lysates.• Regularly performed DNA extractions on high titer lysates.• Analyzed DNA samples via gel electrophoresis and nanodrop for size and purity before submitting samples for sequencing.• Occasionally assisted Saha Lab with Xenopus laevis embryo fixing.• Maintained Xenopus leavis colonies by changing tank water and appropriately feeding frogs according to size and age. -
CashierDelhaize America Jan 2016 - Aug 2016Yorktown, Virginia, United StatesWorked as a part-time cashier during high school at a Food Lion grocery store. -
CashierSupervalu Dec 2013 - Dec 2015Yorktown, Virginia, United StatesWorked as a part-time cashier during high school at a Farm Fresh grocery store.
Daniel Sanchez De La Rosa Education Details
Frequently Asked Questions about Daniel Sanchez De La Rosa
What company does Daniel Sanchez De La Rosa work for?
Daniel Sanchez De La Rosa works for York County Tourism Development
What is Daniel Sanchez De La Rosa's role at the current company?
Daniel Sanchez De La Rosa's current role is Laboratory and Research Specialist.
What schools did Daniel Sanchez De La Rosa attend?
Daniel Sanchez De La Rosa attended Virginia Commonwealth University, William & Mary, Thomas Nelson Community College.
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DANIEL SANCHEZ DE LA ROSA
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