Daniel Selma Herrador Email and Phone Number

"Mechanical Stability of Multicellular Assemblies"Supervisor: Martial BallandCo-Supervisor 1: Thomas BoudouCo-Supervisor 2: Giovanni CappelloMentor: Vladimir Misiak

"Design of an In Vitro System to Study the Mechanical Stability of Cell Quadruplets."Supervisor: Martial Balland (UGA)Co-supervisor: Hans Van Oosterwyck (KUL)Mentor: Vladimir Misiak (UGA)

I did a summer internship simultaneously in 2 different research groups within the MeBioS division (Department of Biosystems, KU Leuven). I worked in the Biosensors group and in the Biomimetics group, on alternate days. I had the chance to do trainings in a clean room, in wide field and fluorescence microscopy, in micro- and nanopatterning with soft lithography and maskless UV exposure methods and in continuous and digital microfludic systems for sorting and multiplexing cells, respectively and among others.Biosensors group. Design and fabrication of microfluidic chips for B cell mining for monoclonal antibody discovery. Under supervision of PhD candidate Julie Van Lent, Dr. Karen Ven & Prof. Jeroen Lammertyn.Biomimetics group. Optimization of microfluidic chips for high-speed image-based erythrocytes sorting. Horizon Europe project SynEry. Under supervision of PhD candidate Max Wolf & Prof. Xavier Casadevall.

Event host in big fairs. Brand ambassador for Android/Google in the Mobile World Congress (MWC) 2018, 2019 & as a Supervisor of 90 hostesses in 2022. Staff at Il·lustre Col·legi de l'Advocacia de Barcelona (ICAB) convention in 2018. Staff at a Bosch convention at World Trade Center Barcelona in 2018. Brand ambassador at the Virtual Machine World (VMWorld) congress in 2017.

Summer teacher in a school with students in risk of social exclusion. My role was to help, guide and motivate students of different ages to pass their retakes whilst entertaining them and understanding all their different backgrounds.

Nanopatterning charaterization for cell-matrix interactions in chondrogenic differentiation of human msenchymal stem cells (hMSCs).ABSTRACTRegenerative medicine and cell therapy are truly promising fields with a great potential thanks to the huge development of nanoscience and nanotechnology. Cells can sense the extracellular matrix binding domains, as well as their density, distribution, stiffness and physicochemical properties. This physicochemical sensing ability allow them to adhere, proliferate and differentiate when some mechanical threshold forces are surpassed or some specific interactions are given, all driven by a mechanochemical transduction path with the extracellular matrix as main character.In this project, tailored RGD dendrimer-based uneven nanopatterns on PLLA films to direct chondrogenic commitment and differentiation are synthesized and characterized. Characterization of the different prepared nanopatterns is carried out by Atomic Force Microscopy (AFM) imaging. In addition, modelling of cell-matrix interactions is first performed by an adapted mathematical model which predicts force and position trends, simulating cell adhesion process. Then, a second characterization is performed by Lateral Force Microscopy (LFM) with and without integrin-on-tip (i-o-t) of the AFM, where surface friction coefficients of nanopatterns are obtained.

I worked as an assistant on a Doctoral Thesis in charge of Prof. Romà Tauler and Prof. Silvia Lacorte. Here I learned how to obtain representative soil and water samples in field works and how to process them from scratch to quantify the presence of drugs and pesticides using GC-MS & HPLC-MS/MS. I also started a project based on macroporous devices and adsorbents to remove pollutants from water. Here I had to deal and ask for free samples of different adsorbents to companies to perform some initial experiments.

I give private lessons to high school students, regardless they study science or letters. This job has been very useful to me in order to learn to explain and teach to students of different ages, putting patience into practice and forcing me to see different perspectives of a same subject. Since I begun I realised how teaching fullfills me.

First whole summer job in 2018 at Sports Bar Sitges, a restaurant with room for 300 people, coinciding with the Football World Cup 2018. I learned how to work fast, under pressure and within a team for long periods of time, as well as to assume responsibilities and main functions since the beginning.