Daniel Yu Email & Phone Number

Towson, MD, US

Baltimore, Maryland, United States

• Led interdisciplinary teams in groundbreaking gene editing and gene therapy projects targeting genetic disorders like CF and FCED, achieving mutation correction through innovative assay development and optimization
• Spearheaded the implementation of a standardized digital PCR quantification pipeline in cell-based gene expression assays, enhancing promoter strength validation and significantly advancing preclinical gene-editing.
• Fostered collaborative partnerships with Johns Hopkins Medical School, contributing digital PCR assays that precisely quantified CRISPR gene-editing efficiency, instrumental in securing funding for biotech startup

Baltimore, Maryland, United States

• Optimized ddPCR and RT-ddPCR assays, improving gene copy number quantification accuracy for CF and FCED studies, demonstrating advanced assay development skills, and contributing to critical diagnostic advancements
• Led the development and implementation of an automated liquid handling system for Luciferase assays across various human tissue cell cultures, enhancing the discovery of compact promoters, thereby broadening the.
• Invented a novel tool to prevent technical errors that commonly occur in ddPCR assays, showcasing innovation and problem-solving skills, and developed a universal, cost-efficient ddPCR solution, significantly reducing.

Baltimore, MD

• Advisor: Sarah Woodson, Ph.D., T.C. Jenkins professorThesis: RNA folding and the fitness landscape of the glmS ribozyme
• Spearheaded the construction of the glmS ribozyme fitness landscape using biochemical assays and NGS, elucidating key mutation tolerance hierarchies and enhancing the understanding of RNA structural domains
• Innovated an HTP sequencing strategy for glmS ribozyme activity, developing a comprehensive sequence-to-function map that underscored the impact of nucleobase alterations on catalytic RNA activity, significantly.
• Authored custom Python scripts for the meticulous analysis of NGS data, uncovering the pivotal roles of long-range tertiary motifs in glmS ribozyme self-cleavage, thereby demonstrating proficiency in Python programming.
• Acquired a Python certification from Coursera: Inferential statistical analysis with Python
• Taught in Protein Engineering and Biochemistry Laboratory for three semesters and mentored two rotation students, three undergraduate students, and one high school student in the lab

Framingham, MA

• Developed and validated a clinical assay for UGT1A1 genotyping, significantly advancing the Gilbert syndrome project by enhancing diagnostic accuracy and patient treatment strategies
• Executed NeoGAA pharmacokinetic assays based on enzymatic activity, contributing to the Pompe program's success by improving treatment efficacy through precise dosage adjustments
• Pioneered the use of ELISA and radio-immunoassay techniques for the quantification of asthma biomarkers and human serum biomarkers, respectively, driving forward clinical trials and research on segmental.

Doctor Of Philosophy (Ph.D.), Biochemistry, Biophysics And Molecular Biology

Cell, Molecular, Developmental Biology, and Biophysics (CMDB) program at Johns Hopkins University Krieger School of Art and Science

Master’S Degree, Cell/Cellular And Molecular Biology

Bachelor’S Degree, Biology/Biological Sciences

Activities and Societies: National Taiwan University Symphony Orchestra (NTUSO)