Daniela Pontieri Email & Phone Number

Copenhagen, Capital Region Of Denmark, Denmark

Copenhagen, Capital Region Of Denmark, Denmark

I am currently developing affinity purification method of cancer exosome.

Copenhagen, Capital Region, Denmark

Providing on-demand assistance for laboratory industry

Copenhagen, Capital Region, Denmark

I played a role in the development of innovative circular RNA therapeutics for treatment of metabolic diseases. I obtained skills in work with in vitro models, protein quantitation and immunoassay, but also in establishing company´s laboratory set-ups and in Benchling implementation.I successfully set up and characterized indication-specific models.

Hørsholm, Capital Region, Denmark

I obtained hands-on skills in perfusion fermentation in order to optimize upscaling process of protein production in Drosophila cell line. I also developed transient and stable insect cell lines via transfections. In addition, I independently developed a pipeline for whole genome sequencing data (Oxford Nanopore Technologies) analysis.

Copenhagen, Capital Region, Denmark

Hørsholm, Capital Region, Denmark

I coordinated company´s administrative tasks and provided organizational and communication support in order to secure colleagues´ smooth daily workflow by daily communication with key suppliers, by securing company´s stock of laboratory materials and office supplies, by organizing computer equipment for new employees, by onboarding of new employees, all in.

I investigated sensory response to the bath applied neurotransmitters (synthetic neuropeptides) via extracellular electrophysiology and expression patterns of visual proteins in box jellyfish via immunohistochemistry.

Copenhagen, Capital Region, Denmark

Cologne, North Rhine-Westphalia, Germany

During my postdoc position I was responsible for RNA-sequencing data analysis of 30 transcriptomes of 4 developmental stages of bug species Oncopeltus fasciatus. I generated list of differentially expressed genes during important developmental events of the embryogenesis.

Cologne, North Rhine-Westphalia, Germany

During my PhD I investigated molecular changes underlying functional diversification of Hox3/zen paralogues in the beetle Tribolium castaneum. I described detailed spatial and temporal expression profiles of the paralogues on both protein and mRNA level. I performed RNA-sequencing after RNA-interference in both early and late embryogenesis. I analyzed.

Copenhagen, Capital Region, Denmark

During my research assistant position I described contrast-dependent visual response of box jellyfish via behavioral ecology assay. I also characterized proliferation rates and patterns in diverse developmental stages of the box jellyfish and metamorphosing polyps using EdU and TUNEL detection assays.

During my short lab technician position I was responsible for husbandry of various animal models: box jellyfish (Tripedalia cystophora), amphioxus (Branchiostoma floridae), medaka fish (Oryzia latipes), sea anemone (Nematostella vectensis), annelid worm (Platynereis dumerilii).

Prague, Czechia

I investigated Bmp signaling in axial cell fate determination of amphioxus using molecular cloning to isolate promoter sequences and genome editing to test functionality of the tissue specific promoters in developing fish embryo. To characterize expression profile of genes of interest I prepared fish embryos for in situ hybridization using microdissection.

Prague, Czechia

I investigated functionality of invertebrate promoters in vertebrate heterologous systems. Via molecular cloning I isolated regulatory regions of housekeeping genes from the amphioxus and cloned them into two sets of reporter vectors. I then tested the functionality of these invertebrate regulatory regions in mammalian cell lines via transfections and in.

Phd Degree, Developmental Biology

Master'S Degree, Cell And Developmental Biology

Bachelor'S Degree, Molecular Biology And Biochemistry Of Organisms