Daniel Brink, Phd Email & Phone Number

Sweden

Sweden

I worked in two different groups for a joint research project: one group at Lund University and one group at Chalmers University, Sweden.At Chalmers, I worked with transcriptomic analysis (RNAseq) of the gene expression response to different levels of nitrogen and sugars in two novel lipid-accumulating yeast isolates. I was responsible for leading the bioinformatics side of the project, designed the work-flow, and performed the in silico work together with a PhD student that I supervised. I co-authored two scientific publications from this collaboration.At Lund University, I led two projects. In the first, I developed a transformation protocol that allowed successful genetic engineering of one of the yeast isolates. In the second, I demonstrated proof-of-concept production of a novel non-native product in the host cells. During this work, I supervised three master students. I also lent my expertise to other project at the division, which resulted in four co-authored scientific papers.I addition to this, I lectured in six different courses at Lund University, giving eight lectures per year.

Lund

1 year post-dissertation extension of my PhD-student projects in which I published the last manuscript from my PhD studies, and finalized a few experiments.

Lund, Sverige

During my doctoral studies I researched different aspects of microbial metabolic engineering for valorization of lignocellulosic biomass. I worked on three different aspects of the topic and co-authored eight scientific publications.One was an interdisciplinary research projects on microbial valorization of lignin - a major part of plant biomass that has historically not been considered as a feedstock in biotechnological applications. This collected microbiologists, chemical engineers, and analytical chemists. My role was to work with genomics, bioinformatics, and database design. I worked with whole-genome sequencing, assembly, and annotation of novel bacterial isolates, discovery of novel enzyme activities, and design, programming and curation of a web-database on the microbial metabolism of lignin and lignin-derived aromatic compounds.The second project concerned analysis of the response of the sugar signalling network in baker's yeasts to the non-native sugar xylose. I constructed fluorescent reporter cassettes that reported on the signal transduction of the network. This was quantified on a single-cell level using flow cytometry. This was a continuation of the work I started when I worked as a project assistant prior to my employment as a PhD student.The third project regarded the genetic analysis of yeast strains that had been evolutionary adapted to better tolerate the inhibitory compounds found in lignocellulosic hydrolysates. Using comparative genomics, I analyzed the changes in genotype that occured during the evolution process and correlated key genetic changes to the novel phenotype.During my doctoral studies I supervised three Master students, taught one laboratory course per year and gave two lectures per year.

Lund, Sverige

I developed in vivo fluorescent protein systems that reported on the state of the sugar signalling network in baker's yeast Saccharomyces cerevisiae. I evaluated the recombinant strains using flow cytometry.

Doctor Of Philosophy - Phd, Applied Microbiology

Doctoral thesis: Understanding and improving microbial cell factories through Large Scale Data-approaches During my doctoral research, I.

Master Of Science (Msc) In Engineering, Biotechnology

Activities and Societies: Secretary of the board of the Student Union of Chemical Engineering and Biotechnology 2011-2012 I specialized.