Daniel Nguyen Email and Phone Number

• Provided cell sort troubleshooting and optimization for mammalian display selection and B cell cloning for Antibody Discovery and Engineering team.• Performed MACS, FACS (analysis and sorting), 10X genomics, cell culture, plasmid cloning, molecular biology.• Collaborated across departments with sorting projects and stable cell line development.• Operation and maintenance of Aria III and Fusion sorter instruments.

• Manage core scheduling and billing responsibilities. Establish new working accounts with internal and external researchers for use of core facility.• Maintain BD FACS Aria II cell sorter equipment and perform routine instrument calibration for optimized performance.• Maintain Invitrogen™ Attune™ NxT flow cytometer equipment and perform routine instrument calibration for optimized performance.• Set up, optimize and run fluorescent-activated cell sorting (FACS) experiments as well as cell population analysis experiments.• Provide consultation for researchers with questions related to flow cytometry and experimental design, including multi-color/multi-parametric analysis.• Train researchers on principals of fluorescence, detection and flow cytometry application. Train researchers how to operate cytometer and software functions.• Communicate with cytometer manufacturers reporting bugs, issues, or new feature requests.• Arrange seminars at Forsyth to educate researchers on new and current flow applications, technologies, and data analysis techniques.• Collaborate with flow cytometry related online services to enhance researcher productivity.

• Investigate periodontal disease pathways, progression, and resolution. Bridging the gap between science and therapeutics through translational research. Routinely analyze clinical samples, cell signaling pathways, gene expression, DNA-DNA hybridization, perform immunoassays, process and analyze histology tissue samples, microbiology culture, and in vitro cell culture.• Manage laboratory practices, standard operating procedures, equipment operation training, maintain equipment servicing and calibration, etc.• Lab supply management and project reagent/material sourcing.• Lab safety representative, waste flow management, emergency preparedness, and emergency response. CPR certified.• Training of visiting scientists, clinicians, students, and research scholars on safe lab practices and experimental protocols.• Collaborate with external and internal scientists in sample analysis, training methodologies, and consultation.• Supervise lab operations, experiments, and provide assistance/consultations to researchers.• Collaborate with equipment manufacturers and lab supply vendors in testing new methods/products for laboratory research.• Presenter at American/International Association for Dental Research Conference (2010, 2012, 2013, 2014 and 2015)• Co-Author of "ERV1 Overexpression in Myeloid Cells Protects against High Fat Diet Induced Obesity and Glucose Intolerance." Sci Rep. 2017• Co-Author of "Strain-Specific Impact of Fusobacterium Nucleatum on Neutrophil Function." J Periodontol. 2017• Co-Author of "Dental plaque microbial profiles of children from Khartoum, Sudan, with congenital heart defects." J Oral Microbiol. 2017• Co-Author of "Endothelial Cell response to Fusobacterium nucleatum." Infect Immun. 2016 • Co-Author of "Maresin 1 Biosynthesis and Pro-resolving Anti-Infective Functions with Human Localized Aggressive Periodontitis Leukocytes." Infect Immun. 2015