Danielle D'Erminio Email & Phone Number

Cincinnati, OH, US

Cincinnati, Ohio, United States

New York, New York, United States

• PhD thesis research performed at Mount Sinai utilized a neonatal mouse model with intervertebral disc (IVD) puncture injury to identify cellular and matrix factors influencing IVD regeneration.
• Additional work Investigated the effects of a high-fat diet on the murine spine and the role of advanced glycation end products (AGEs) and AGE receptors including the receptor for advanced glycation end-products (RAGE).
• Gained experience with mouse models using both ex-vivo organ culture and in-vivo methods including performing orthopedic surgeries on live mice
• Confident communicator of data and technical information to internal colleagues and external audiences at national conferences resulting in 20+ oral presentations
• Biweekly meetings and collaboration with orthopedic spine surgeons at Mount Sinai
• Implemented structured inventory and sample management system and taught improved project organization skills to fellow lab members gained from previous industry experience

• Attended graduate-level classes to prepare for future PhD programs

200 Technology Square, Cambridge MA

• Managed research projects at a fast-paced and dynamic company within a core facility to advance R&D research
• Worked with cross-functional teams learned 15 new technical skills
• Delivered project-specific status reports to discuss characterization of various mRNA products
• Ensured compliance and maintain health and safety while leveraging principles of Good Laboratory Practices (GLP)
• Gained experience in RNA isolation and detection methods including fluorescence-based Ribogreen assays, RT-qPCR, nanodrop, and qBIT assays
• Worked on protein separation techniques including size exclusion chromatography using HPLC, sucrose-based gradients, and immunoprecipitations using FLAG tags

Wilmington, North Carolina

• Detailed record-keeping skills using electronic lab notebooks during compendial testing of active pharmaceutical ingredients with regulatory requirements set by the U.S., Japanese, and European pharmacopoeias
• Trained in the following techniques: heavy metal limits testing, arsenic limits testing, specific gravity, bulk density, melting point, pH, viscosity, optical rotation, Fourier transform infrared spectroscopy (FTIR).
• Extensive supply and inventory monitoring to ensure proper testing of pharmaceutical products

University Of Alabama

• Characterizing allosteric mechanisms in multi-domain enzymes
• Inserting genes into vectors, cloning, PCR, protein purification
• Compared sequence identity, protein similarity, kinetic and inhibition parameters, and Ki, Kcat, and Km values of IPMS isolated from Francisella novicida (FnIPMS) and from Mycobacterium novicida (MtIPMS)
• Presented a poster highlighting findings that are contained in a pending manuscript at the South Eastern Enzyme Conference in Atlanta, Georgia in April of 2013
• Familiar with micropipettes, balances, FPLC, pH meters, sonicator, UV spectrometer, autoclave

Boston, MA

• Researched a mitochondrial enzyme target for potential cancer treatment
• Performed and completed an assay development project (Resazurin-Diaphorase)
• Performed protease and ATPase assays as well as IC50 calculations and western blotting
• Gained experience working on a Biacore and plate readers
• Presented findings at weekly project meetings
• Worked in GMP environment and contributed to the creation of an SOP procedure for resazurin-diaphorase assay

Phd, Biomedical Engineering

Bs, Chemical Engineering