Danielle Large Email & Phone Number

Boston, Ma, Us

Philadelphia, Pennsylvania, United States

Greater Boston Area

• Synthesize, functionalize, and characterize liposomes for therapeutic delivery applications• Dissection, dissociation, and culturing of rat dorsal root ganglia (DRG) neurons• Perform experiments using DRG neurons including: nanoparticle uptake, immunocytochemistry, and evaluate drug (paclitaxel, doxorubicin) effect on growth morphology & metabolism.

Cambridge, Ma

• Performed various assays to evaluate the safety of therapeutic antibodies • Optimized ELISA protocol to determine protein levels in mouse serum samples • Examined the effects of chemotherapy on the activity of immunotherapeutic antibodies

Knoxville, Tennessee Area

• Manufacture specified agar gels for cell culture and growth• Culture bacteria in an environment with selective pressure• Manufacture, electrophorese, and analyze acrylamide gels

Knoxville, Tennessee Area

• Performed calibrations on pressure, wind, and temperature transmitters to be within industry standard using NIST certified instruments.• Traveled to power generating stations conducting tests to verify that the client meets performance requirements per contractual agreements and emissions per EPA requirements. Performed field installation of instrumentation, hookup of Agilent Technologies data acquisition system and system checkouts. • Monitored and recorded instrumentation during tests normally performed in twelve hours shifts. Supported both day and night shifts depending on location.• Packed, weighed, and shipped necessary equipment for external projects.

Knoxville, Tennessee Area

• Performed cell culture techniques, including: passing cells from one generation to the next, resurrected cells stored in liquid nitrogen, and counting, diluting, or concentrating the cell density of a solution based upon need of a specific experiment.• Cultured various lines of human cancer cells, including: SW480, HCT116, and HT29.• Performed the application of micro-amperage to cells attached to the nano-circuitry apparatus.• Retained images of cell assay through use of IVIS imaging machine in order to compile pictures and data.• Trained and certified in BSL2 Lab Safety.

Phd, Bioengineering

Bs, Chemical Engineering

Activities and Societies: Society of Women Engineers- Mentor and Social Committee Chair

Bs, Biochemistry And Molecular Biology

Activities and Societies: Phi Eta Sigma Honor Society Relay for Life Exec Committee 2010-2011