Danielle Lewis Email & Phone Number

SENIOR RESEARCH SCIENTIST, Marker Diagnostics Ltd., Birmingham,Conducting advanced laboratory experiments, data analysis and evaluation, data presentation to external teams, liaising with customers and product sales teams, training staff and student volunteers, Research and development, product validation, trained by Thermofisher in qPCR with SYBR and TaqMan based products. Expert knowledge in lncRNA and sncRNA including; miRNA, tRNA, SNORA, snoRNA, piRNA. Experience with mRNA -GAPDH, RPL4. NDUFA1.Currently undergoing industrial PhD. MSc in Blood science. BSc in Biochemistry.FORMER LABORATORY MANAGER, Marker Diagnostics Ltd., Birmingham, 2022-2023 *Implementation and management of SOP’s, Risk assessments and COSHH, managed a small team, managed and trained BSc and MSc students, Implemented and managed a LIMS system, implemented a barcoding system for sample management, document control, Implemented and conducted auditing and quality management systems. Equipment management: servicing and maintenance, Stock control and ordering.FORMER RESEARCH TECHNICIAN, Inflammation and aging, University of Birmingham, 2019-2022*Laboratory techniques including: Flow cytometry on immune cells using multiple markers eg CD14/CD64, ELISA, Luminex multiplex, immunoassays, chemotaxis, cell isolation, migration assays with macrophages and neutrophils, confocal microscopy, cell cultures, ion chromatography, ICP spectrometry, photometry, SDS PAGE, thin layer chromatography, gel electrophoresis. Understanding immune cells to a deeper level in diseases of ageing, including stroke & frailty - expertise in cell experiments including flow cytometry, elisa, luminex(multiplex), confocal microscopy and chemotaxis chamber methods. Medical Laboratory technician, University Hospitals Birmingham, 2018-2019Industrial Laboratory technician, Eurofins, 2016-2017Chemistry Laboratory technician, HydroViron Water Treatment, Graduation-2016* References available upon requestLab/Technical skills•including aseptic practice, elisa, immunoassays, ion chromatography, ICP spectrometry, photometry, microscopy, SDS PAGE, PCR, SYBR and TAQMAN PROBE based qPCR, Poly(a) tailing, Reverse transcription/cDNA conversion, thin layer chromatography, DNA and RNA extraction, gel electrophoresis, immunoblots, Flow Cytometry, cell isolation, cell culture). I have worked closely with neutrophils and macrophages and supported BSc students with their dissertation projects.