Daniel Peterson Email & Phone Number

Chicago, Il, Us

Libertyville, Illinois, United States

Contributed to development, validation, and integration of internal bioinformatics pipelines for the Synthetic Biology department. Provided support and leadership for research projects/operations as SynBio leadership team member, and managed projects of direct report scientists. - Next Generation Sequencing (NGS) data processing and assembly (de novo and re-sequencing) for DNA of model and non-model organisms using reads from Sanger, PacBio, Illumina, and ONT technologies. - Gene prediction, annotation, alignment, and metabolic mapping.- RNAseq data processing and analysis for differentially expressed genes (DEG) and visualization of results in R/Python (ggplot2 and seaborn). - Public database mining/searches using both prebuilt and developed APIs and scripts.- Orthologous gene analysis, biosynthetic gene cluster (BGC) identification, soil metagenomics processing, 16S and multi-locus sequence identification (MLSA), and other pick-up tasks as needed. - Workstation/server administration and maintenance, user environment handling, package installation.- AWS Cloud resource administration and maintenance (S3, EC2, Lamda)- Script development and validation in R, Python, Shell, Perl and code versioning (GitHub).- Coordinated integration of new automated liquid handler instrumentation and operations for increased throughput of molecular lab based procedures. - Development of RT-qPCR protocols for gene expression. - ISO 9001:2015 Internal Auditor.

Libertyville, Illinois, United States

Contributed to development of procedures for the Synthetic Biology department. Supporting metabolic engineering projects, working on both model and non-model organisms.- Integrative/replicative plasmid design (BioPython, Geneious Prime, and SnapGene).- Plasmid assembly/construction (Gibson, golden-gate, ligation). - E. coli and yeast transformation (chemical, electroporation).- E. coil protein heterologous expression, purification, and SDS-PAGE analysis.- E. coli conjugation transformation of non-model organisms.- DNA/RNA isolation, purification, quantification.- PCR amplification, including difficult and high-GC targets.- PCR multiplex development for diagnostic needs. - RT-PCR for gene expression. - Sanger sequencing confirmation of strain engineering.- Growth, maintenance, and cultivation of model and non-model strains.- Communication/presentation of results.- Ordering and stocking supplies, equipment maintenance.- Guiding newly hired research associates.

Skokie, Illinois, United States

Conducted automated RNA extraction and qRT-PCR testing for COVID-19 infection in nasopharyngeal patient samples. -RNA/DNA extraction using Tecan Fluent 780 automated liquid handler.-qRT-PCR plate (384 well) setup using Tecan Fluent 480. -qPCR analysis of results using Applied Biosystems QuantStudio 12k. -Managed and trained multiple laboratory technicians and technologists for basic laboratory duties and procedures. -Wrote and optimized SOPs and workflows for completion of daily tasks. -Developed Windows batch scripts and Excel workbooks for tracking of workflow pipelines.

Dekalb, Il

Conducted wet-lab and computational (bioinformatics) research for the Northern Illinois University Department of Biological Sciences.-Wet-lab techniques for bacterial transformation, fungal transformation, toxin analysis using thin layer chromatography, polymerase chain reactions (PCR) for diagnosis and recombination, restriction enzyme digestion and ligation.-Computational bioinformatics analyses using large biological datasets with Linux/Unix, Perl/Python/R/Shell Scripting, NGS tools, sequence alignment tools, statistical analyses pipelines, and data visualization techniques.-Set-up, maintained, and provided hands-on troubleshooting of lab bioinformatics server.-Provided direct instruction for Bacteriology BIOS 213 laboratory sections for microscopic staining procedures, microbial physiology/morphology, culturing of aerobic and anaerobic bacteria, and identification of unknown bacteria through biochemical tests.

Dane County, Wi

Studied heavily in techniques and procedures for DNA analysis, DNA profiling, and forensic sciences.-Graduated 720 hour Law Enforcement Academy - Blackhawk Technical College-Processed persons booked into jail.-Maintained security of inmates and monitored health and welfare.-Observed, recognized and took appropriate actions on suspicious activity.-Responded to calls for emergency service.-Assisted with mental health crisis.-Used communication skills to de-escalate conflicts and potentially violent situations.

St. Charles, Il

Performed automated quantitative chemical analyses of specimen samples to provide information used in the diagnosis and treatment of disease. -ELISA stool calprotectin (fCAL) testing both manually and using a Hycor Hytec liquid handler, -IgA/IgG tTA/tTG dried blood spot and blood serum testing using an INOVA BioFlash chemiluminescent analyzer.-Microscopic analysis for stool fat content and testing for pH and occult blood. -Calibrated and maintained equipment, identified problems and enacted corrective action, and closely adhered to the quality control policies and procedures.

St. Charles, Il

Completed preparations of hair, blood, urine, and stool samples for chemical analysis.-Nitric acid digestion of specimen samples for heavy metal quantitation.-Hematocrit testing for red blood cell count. -Scheduled specimen tests, helped optimize workflows, prepared reagents, kept accurate documentation, and monitored inventory.

Master Of Science - Msc, Biological Sciences, Bioinformatics

Activities and Societies: Python Society Member

Bachelor Of Science - Bsc, Biological And Biomedical Sciences

Activities and Societies: Pre-Professional Association Member, West Campus Council Ambassador, Saluki Paintball Club President