• Highly motivated cell biologist with 14 years of scientific research experience, including 4 years of postdoctoral training and 1.5 years of industry experience. In-depth knowledge of Natural Killer (NK) and T cell biology is evidenced by multiple first-author publications. • Substantial hands-on experience in methods of cellular and molecular biology, immunology, and biochemistry. • Able to work independently and in collaboration, design and perform experiments, analyze and present data, and write scientific grants and manuscripts as evidenced by NIH fellowships and other financial awards.TECHNICAL SKILLS• In vitro:Cell culture: generation, expansion, characterization, cryopreservation of cell lines and primary cellsImmune cell (T and NK cell) isolation from peripheral blood and organs, co-culture, labeling, depletion, phenotyping, functional assays (cytokine production, cytotoxicity, proliferation)Multi-color flow cytometry: surface and intracellular staining, sample acquisitionCell transfection: transduction, electroporation, lipofectionMetabolic flux assay: Seahorse• In vivo:Routes of administration: intraperitoneal (IP), intravenous (IV), intragastric (IG) and subcutaneous (SC)Mouse handling: care, breeding, genotypingTissue processing: spleen, lymph node, intestine, peritoneum, lung, liver, brain, blood• Molecular biology:DNA and RNA: extraction, molecular cloning techniques using bacterial expression vectors, PCR, qPCR, RT-PCR, transfection, electroporation, siRNAProtein: SDS-PAAG electrophoresis, western blot, ELISA, multiplex immunoassays, antibody purificationMicroscopy: light and confocal• Data analysis: FlowJo, GraphPad Prism, Excel, LEGENDPlex• Computer skills: Microsoft Office, Adobe Illustrator