Darrell Lockhart Email & Phone Number

United States

-Assisted in developing protocols for extracting nucleic acids from air and surfaces for subsequent pathogen detection using qPCR assays-Researched and optimized internal control standards for in-house DNA gel electrophoresis and qPCR assays-Led the development of in-house microbial qPCR assays for quantifying eukaryotic and prokaryotic genomic DNA in various biological sample types.-Evaluated nucleic acid extraction buffers, magnetic nanoparticle bead chemistry and led the validation of an in-house magnetic bead DNA size-selection kit.-Trained new employees on RNA/DNA nucleic acid extraction and DNA size-selection protocols-Assisted in troubleshooting general laboratory protocols and specialized in magnetic nanoparticle workflows

Seattle, Washington, Us

Seattle, Wa, Us

-Produced significant numbers of Adeno-Associated Virus preps for use in Seattle Children’s Research Institute’s Center for Immunity and Immunotherapies research-Corresponded with department collaborators to ensure the production of optimal AAV product-Developed and prepared complex cell co-cultures to establish cytotoxic avoidance in genetically engineered cells-Utilized lentiviral transduction to generate experimental/control cell lines-Researched and developed cloning strategies with bioinformatic software to established optimal vectors for B cell therapies-Performed immunoassays to analyze antibody secretion in-vivo mouse models-Performed mammalian and primary cell culture, while employing techniques such as CRISPR-Cas9 gene editing and lentiviral transduction for in-vivo and in-vitro experimentation-Conducted research data analysis, and communicated findings to department and collaborators

Principal Investigator: Stan Langevin, PhD.-Performed high-throughput nucleic acid extractions and qPCR analysis on sewage samples for a CARES grant COVID-19 pandemic research collaboration with the Pierce County Department of Health-Performed high-throughput nucleic acid extractions and qPCR analysis on human mucosal, nasopharyngeal, salivary samples -Assisted in developing a commercial multiplex qPCR assay for detection of SARS-CoV2 RNA in various biological sample types.-Designed and optimized primer/probe sets used for extraction controls in in-house qPCR assays-Trained new employees on aseptic technique, BSL-2 lab procedures, RNA/DNA nucleic acid extractions and qPCR protocols-Assisted in troubleshooting involving insufficiency with in-house assays

Principal Investigator: Ana Groat-Carmona, PhD.-Used molecular based techniques such as molecular cloning, PCR, and gel electrophoresis analysis to modify the Plasmodium BEM46-like protein in malarial parasites in order to identify and study proteins on the Plasmodium parasitic plasma membrane-Demonstrated competency in performing an array of experiments in BSL-1 and BSL-2 environments-Successfully performed numerous dissections of Anopheles mosquitoes and extractions of malarial parasites