Personalis is a genome-scale diagnostics company pioneering genome-guided medicine. Personalis Genome Services provides clinicians and researchers accurate comprehensive end-to-end human genome sequencing and interpretation solutions in Cancer, Immuno-oncology and Inherited Disease. • Responsible for for managing the marketing activities for the continued development and commercial support of the Personalis Services for the Cancer Immune Response portfolio. • Includes all aspects of the product life cycle from product development through end of life.

Led product management for Ingenuity’s clinical decision support software products and services that guide genomic variant interpretation and clinical reporting for somatic and hereditary cancer.• Launched QIAGEN Clinical Insight, exceeding revenue goals by 50%.• Defined customer and product requirements for software and content, guiding plan of record.• Specified key messages for marketing team, driving awareness and demand over 3 campaigns.• Managed clinical beta program, confirming product fit and acquiring testimonials for collateral.• Drove product adoption and benefit success through usage reports and customer feedback.

Ion Reporter is a suite of bioinformatics tools that streamline and simplify data analysis, annotation, and archiving of Ion semiconductor sequencing data.• Created and commercialized Ion Reporter Software and Server with targeted assays for solid tumors.• Specified customer-driven requirements for sequencing and analysis pipelines in cloud-based and locally-deployed environments for research and clinical applications, particularly in oncology.• Managed product roadmap and directed diverse R&D teams of 70 people over three continents through six product release cycles.• Developed beta and early access programs to gather customer feedback and develop interest.• Built outbound marketing campaign; trained technical staff, sales, and customer support; executed with team members in Sales, Market Development, Legal, Finance, and Operations.• Received two internal awards for product leadership and market launch.

• Directed development and integration of data standards: formats, annotations, and messaging.• Collected and summarized customer use cases and feedback through focus groups and customer interviews on common DNA and RNA sequencing applications to define core workflows and customer-specific customization• Generated customer and product requirements for LifeScope Software development and launch.• Elected Co-Chair for HL7 Clinical Genomics Work Group to initiate clinical sequencing standards.

DNAnexus provides cloud-based solutions for high throughput sequence data management, analysis, and visualization. This provides an end-to-end solution for biological researchers to extract information from sequence data without the bioinformatics and IT challenges.

Navigenics was a personal genomics company aimed at empowering individuals to reduce their disease risk by developing products to reveal genetic predisposition for important health conditions.• Development of the Genetic Composite Index (GCI) to estimate the association of a condition with the combined effect of a set of genetic variants.• Development of Affymetrix GW 6.0 and Applied Biosystems TaqMan Genotyping assays with validation against reference HapMap samples and customer saliva samples.• Analysis of complex genomic regions (e.g., HLA and APOE) using linkage disequilibrium to identify assayable variants across all populations.• Integration of disease-level data (heritability, prevalence, life time risk) with locus-specific details (risk/non-risk alleles, genotype frequencies, odds ratios) to support GCI calculations for all conditions in our products.• Creation and development of multiple reference databases and interfaces.• Built laboratory quality control metrics into the informatics pipeline for early identification of data generation issues and improve data accuracy.

Advisors: David Haussler, John Tamkun, and W. James KentDissertation Title: Using Variation and Constraint to Understand Functional Elements in the Human Genome

Advisors: David Haussler, Carol Rohl, and W. James KentThesis Title: Sequence Variation in the UCSC Genome Browser

Developed client/server tools to manage query sequences, perform genomic mapping, index results for fast retrieval, and display the results with related annotations in a database-driven GUI browser.• Curation and data cleaning of genomic annotations.• Developed a semi-automated pipeline for sequence processing, including acquisition, repeat masking, primer design, primer ordering, and primer tracking. (See patent.)

• Management and analysis of data generated by the Polymorphisms group.• Liaison to internal colleagues and to external clients and collaborators.• Analyze chip image data for SNP identification with custom algorithms.• Developed methods for improving data access and created tracking dashboards.• Coordinated ongoing and retrospective analyses of data quality and validation rates to identify inconsistencies in data quality and identify sources of variability.• Trained users on in-house software and worked with developers to make functional enhancements.

• Purified and characterized antigens for immunization and antibody screening.• Developed IgG quantitation ELISA to increase sensitivity and decrease cost.• Established tissue culture facility and selected optimum growth conditions for hybridoma cell lines by IgG quantitation ELISA and antigen binding ELISA. • Screened mammalian polyclonal antisera for antibody specificity and titer.

Used molecular biology and biochemistry techniques to explore questions in signal transduction and transcriptional regulation.• Biochemical purification and cloning of the transcription factor NF-ATc and paralogs.• Characterized NF-ATc clones by RNAse protection, Northern and Southern blot analysis, eukaryotic transient transfection, reporter gene assays, cytosolic and nuclear protein extraction, and electrophoretic mobility shift assays.• Curated and submitted nucleotide sequence data, searched databases for homologs and orthologs using BLAST, evaluated similarity using ALIGN.• Created monoclonal antibodies using recombinant protein purification, mouse immunization, antiserum characterization, and Western blots.

• Increased synthesis capacity three-fold to over 400 custom oligonucleotides per month.• Developed processes and protocols for new reagents and instrumentation.

Developed protocols for synthesis of Biotin-ON phosphoramidite and related reagents to incorporate chemical labels into synthetic oligonucleotides.