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Scientific leader with over 15 years of experience in early and translational development of biologics and genetic medicines. Effective, solution oriented leader able to push projects forward under tight deadlines.Bioanalytical: Develop strategy and lead method development and assay validation. Management of contract labs and solving operational issues. Development of assays to support biodistribution and toxicology studies: Analysis of proteins (LBAs - ELISA, MSD, multiplex IF/IHC), nucleic acids (quantitative/digital PCR, ISH), immunogenicity (anti-drug antibody assays, cytokine biomarkers). Authorship and review of relevant sections of IND/CTA submissions.Protein engineering/directed evolution: Phage and yeast surface display, recombinant protein production and purification, protein biochemical and biophysical characterization.
Recode Therapeutics
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Principal Scientist, Bioanalytical Assay DevelopmentRecode Therapeutics Apr 2022 - PresentMenlo Park, California & Durham, North Carolina, Us
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Senior Scientist Ii, Bioanalytical Assay DevelopmentRecode Therapeutics Jul 2020 - Mar 2022Menlo Park, California & Durham, North Carolina, Us
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Senior Scientist IRecode Therapeutics Feb 2016 - Jun 2020Menlo Park, California & Durham, North Carolina, Us
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Senior Scientist And Group Leader - Protein EngineeringProtelica Jan 2011 - Feb 2016Project lead for our Pronectin antibody mimetic platform since its inception. Responsible for all aspects of the program: From library design, construction and screening strategies to production and characterization of lead candidates.• Led from the bench project teams that successfully produced lead Pronectin candidates for a wide range of therapeutic and diagnostic applications.• Project manager for a collaborative research project with an industry partner. Project successfully generated Pronectin antagonists for a family of cell surface receptors and generated >$1M revenue.• Co-authored and managed successful Phase I and II NSF SBIR grants that produced >$750K in funding.
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Staff ScientistProtelica Mar 2007 - Jan 2011• Designed optimized phage and yeast display systems for a novel protein scaffold that allowed for rapid identification of high affinity binders after only 2 to 3 rounds of screening.• Developed purification methods for both high-throughput and large-scale production of candidate Pronectins. • Characterized Pronectin binding affinity, stability and activity to rapidly identify lead candidates.• Produced site-specific, chemically modified Pronectins (biotinylation, PEG, fluorescent tags) for in vitro and in vivo applications.
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Postdoctoral Research AssociateSalk Institute For Biological Studies Dec 2002 - Feb 2007La Jolla, Ca, UsInvestigated the mechanism by which TGF-beta signaling induces the growth control genes p15 and p21 and examined the epigenetic silencing of these genes in cancer.• Developed an in vitro system using recombinant and purified proteins to examine recruitment of DNA and histone methylation to a chromatin-assembled p15 promoter. Purified and characterized DNA and histone methyltransferase activities from HeLa cells. -
Postdoctoral FellowYale University School Of Medicine Jan 2001 - Nov 2002New Haven, Ct, UsExamined the role of the lysosomal calcium sensor Synaptotagmin VII (Syt VII) in endosome-lysosome fusion and survival of intracellular bacterial pathogens.• Demonstrated that Syt VII is not required for normal endocytic traffic to lysosomes but is required for a novel mechanism by which cells promote phagolysosomal fusion and limit the growth of intracellular pathogenic bacteria. -
Graduate Student / Postdoctoral FellowUcla Jun 1994 - Dec 2000Los Angeles, Ca, UsInvestigated gene regulation in an early-evolving protozoan human parasite.• Co-developed the first transfection methods and reporter gene assays for this parasite.• Characterized the first promoter sequences from this parasite and determined that a conserved Initiator element, similar to its mammalian counterpart, is required for transcription start-site selection.• Biochemically isolated and characterized the first DNA-binding transcription factor from this unique organism. -
Research AssociateSystemix Sep 1991 - Sep 1993As part of the assay development team, used PCR, ELISA and flow cytometry-based assays to evaluate anti-HIV compounds in the SCID-hu mouse model.
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Quality Control AnalystGenentech Dec 1990 - Sep 1991South San Francisco, California, UsPerformed quality control assays on final vial and production samples. Analyzed and reported data according to GLP guidelines.
David Liston Skills
David Liston Education Details
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UclaMicrobiology And Immunology -
Uc San DiegoMolecular Biology
Frequently Asked Questions about David Liston
What company does David Liston work for?
David Liston works for Recode Therapeutics
What is David Liston's role at the current company?
David Liston's current role is Principal Scientist, Bioanalytical Assay Development at ReCode Therapeutics.
What is David Liston's email address?
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What is David Liston's direct phone number?
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What schools did David Liston attend?
David Liston attended Ucla, Uc San Diego.
What skills is David Liston known for?
David Liston has skills like Protein Engineering, Directed Evolution, Phage Display, Yeast Display, Molecular Biology, Protein Expression, Protein Conjugation, Protein Protein Interactions, Hplc, Fplc, Chromatography, Cell Biology.
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