Molecular And Enzymology Lab Technician
Waterloo, Ontario, Canada
- The Rose Lab researches human gut microbiota proteins, their roles in starch utilization, andas future diabetes treatment options. In this role, David has:
- Cloned genetic material encoding proteins using PCR techniques and created recombinant plasmids using restriction enzymes and DNA ligase.
- Transformed plasmids containing protein coding genes into both Pichia pastoris yeast and Escherichia coli bacteria using electroporation techniques.
- Created media, buffers, and used microbiology spread plate techniques to grow colonies of single celled organisms.
- Used Pichia pastoris yeast and Escherichia coli bacteria to express, purify and isolate MGAM, SI and BT_3302 enzymes for activity assays and crystallization.