David Driscoll Email and Phone Number
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David Driscoll personal email
David Driscoll is a Research Scientist I at Broad Institute of MIT and Harvard. He possess expertise in western blotting, mammalian cell culture, yeast culture techniques, polymerase chain reaction, transfection and 3 more skills.
Broad Institute Of Mit And Harvard
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Research Scientist IBroad Institute Of Mit And HarvardCambridge, Ma, Us
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Postdoctoral AssociateMassachusetts Institute Of Technology Apr 2024 - Present -
Phd CandidateMassachusetts Institute Of Technology Sep 2017 - Apr 2024Cambridge, Massachusetts, United States- Principal Investigator: Dr. Stephen P Bell- Studying mechanism of helicase loading using in vitro biochemical assays and in vivo yeast assays- Purify numerous proteins using AKTA FPLC system for use during in vitro DNA replication biochemical assays- Optimize complex in vitro assays involving many proteins in DNA replication- Use fluorescently labeled proteins in single molecule TIRF microscopy assay in collaboration with Gelles Lab at Brandeis- Analyze large datasets using MATLAB for single molecule experiments
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Graduate Research InternPfizer Sep 2022 - Dec 2022Cambridge, Massachusetts, United States- 10 hour per week internship to determine effect of inhibition of signaling pathway on downstream phosphorylation and gene expression using cell-based immunofluorescence assay- Extensive work with mammalian cell culture: endothelial (TIME) and myoblast cell lines (c2c12)- Maintained detailed records of experiments using online lab notebook system- Presented work to team, collaborating with mentors -
Research AssistantUniversity Of Rochester Medical Center Jan 2015 - May 2017- Principal Investigator: Dr. Eric Phizicky, PhD- Worked with S. cerevisiae strains containing suppressor mutations- Transformed yeast strains and assayed strains for complementation- Conducted mating test to confirm complementation results- Isolated genomic DNA of strains and amplified gene of interest by PCR to confirm mutations by sequencing- Presented data at weekly lab meetings -
Summer Intern - Cardiometabolic DiseaseMerck Jun 2016 - Aug 2016Boston, Massachusetts- Supervisors: Dr. Min Lu, MD PhD and Dr. Salvatore Iovino, PhD- Validated targets in a muscle phenotypic screen for inducers of fatty acid oxidation by siRNA transfection- Supported efforts of the lab in diabetes early discovery in the context of lipid metabolism and insulin sensitivity- work with immortalized myoblast cell line (c2c12) and primary human myoblasts- Quantified mRNA/protein levels of targets by qPCR/Western Blot -
Summer Research InternBoston University School Of Medicine May 2015 - Aug 2015- Principal Investigator: Dr. Paul Pilch, PhD- Conducted assay measuring adipokine secretion after transfection of novel ER oxidoreductase- Measured secretion levels by Western Blot- Generated mutant protein through site-directed mutagenesis- Presented data to lab at end of internship -
Teaching Assistant - BiologyUniversity Of Rochester Aug 2014 - Dec 2014- Led weekly workshops and held frequent office hours- Graded weekly assignments and exams
David Driscoll Skills
David Driscoll Education Details
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Biology/Biological Sciences, General
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Biochemistry
Frequently Asked Questions about David Driscoll
What company does David Driscoll work for?
David Driscoll works for Broad Institute Of Mit And Harvard
What is David Driscoll's role at the current company?
David Driscoll's current role is Research Scientist I.
What is David Driscoll's email address?
David Driscoll's email address is dd****@****mit.edu
What schools did David Driscoll attend?
David Driscoll attended Massachusetts Institute Of Technology, University Of Rochester.
What skills is David Driscoll known for?
David Driscoll has skills like Western Blotting, Mammalian Cell Culture, Yeast Culture Techniques, Polymerase Chain Reaction, Transfection, Bacterial Transformation, Yeast Transformation, Qpcr.
Who are David Driscoll's colleagues?
David Driscoll's colleagues are Isaac García Reyes, Akshaya Ravi, Kevin Bullock, Jade Moon, Allison Brookhart, Emily Leonard, Tianling Ou.
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David Driscoll
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