David Fine Email & Phone Number

Seattle, WA, US

Seattle, Washington, United States

Seattle, Washington, United States

Perform numerous molecular biology techniques PCR, qPCR, molecular cloning, DNA/RNA isolation, Southern blot, and Sanger sequence analysisDevelop and publish assays for PCR amplification and Sanger sequencing of gene-edited pluripotent stem cellsDesign primers and probes for PCR, qPCR, and Southern blot using BLAST/BLAT genome browsers and Primer3 Plus.

Pittsburgh, PA

 Perform stereotaxic surgery procedures in anesthetized rodents such as aseptic brain injury surgery and fast scan cyclic voltammetry for in vivo dopamine neurotransmission data collection Outline novel characterizations of functional impairments after ischemic brain injury as well as advance pharmacological treatments. Research findings are being used.

Pittsburgh, Pennsylvania

Maintained and ensured integrity of several studies on topics ranging from type 1 diabetes to late-life depression and anxiety Processed both structural and functional data acquired from MRI scans using a wide range of imaging processing software Wrote, ran and provided technical support for software scripts that automate imaging and neuropsychological.

Performed in vivo and in vitro data collection to characterize the cellular and molecular role of Pituitary adenylate cyclase activating protein (PACAP). Dr. May's team also coordinated efforts with behavioral neuroscientists in the Psychology department to explore the role of PACAP in stress and anxiety Performed radio-immunoassays and ELISA to quantify.

Aided in behavioral neuroscience experiments exploring the role of PACAP in stress and anxiety Collected real-time in vivo data in rats  Assisted with tissue perfusions and various surgical procedures such as PACAP peptide infusions Completed descriptive data analysis and statistical modeling Maintained general laboratory solutions stock

Master Of Science - Ms, Neuroscience

Biochemistry, Neuroscience

B.S. Biochemistry