David Fine Email & Phone Number

Seattle, Wa, Us

Seattle, Wa, Us

Seattle, Washington, United States

Seattle, Washington, United States

Perform numerous molecular biology techniques PCR, qPCR, molecular cloning, DNA/RNA isolation, Southern blot, and Sanger sequence analysisDevelop and publish assays for PCR amplification and Sanger sequencing of gene-edited pluripotent stem cellsDesign primers and probes for PCR, qPCR, and Southern blot using BLAST/BLAT genome browsers and Primer3 Plus bioinformatics softwareRoutinely carry out high throughput qPCR, including the use of liquid handling equipmentWrite and execute automated scripts (Shell, R, Python, VBA) to streamline data processingOrganize and manage genomic DNA repository and associated database

Pittsburgh, Pa

 Perform stereotaxic surgery procedures in anesthetized rodents such as aseptic brain injury surgery and fast scan cyclic voltammetry for in vivo dopamine neurotransmission data collection Outline novel characterizations of functional impairments after ischemic brain injury as well as advance pharmacological treatments. Research findings are being used to treat cardiac arrest patients in the Pittsburgh area Collaborate with clinical research team to corroborate data-driven, predictive statistical modeling of brain injury outcomes with preclinical studies, which aided in the acquisition of a patent for specialized immunotherapies to treat TBI Perform biochemistry, molecular biology, and histology techniques such as multicolor flow cytometry, immunohistochemistry, western blots, and ELISA Carry out dissections of animal tissue as well as tissue preservation practices including perfusions, embedding, and mounting Train and mentor over 20 student researchers in neuroscience, immunology, histology data collection and analysis, and scientific writing Write and troubleshoot MATLAB scripts for method automation including analysis of dopamine neurotransmission kinetics Optimize methods for running 12-15 behavioral assessments across two different animal models Complete descriptive data analysis and statistical modeling, and prepare materials for presentations, manuscripts, and grant proposals Organize and manage bio-sample repository and database for both storage and biomarker analysis  Contributed to writing and submitting of two research articles and four abstracts to various scientific conferences and academic journals including Journal of Neurochemistry, Journal of Neurotrama, and ACS Chemical Neuroscience Compile administrative materials and provide literature reviews and/or pilot data for grant applications Oversee general laboratory practices like writing/modifying experimental protocols, maintaining inventory, and preparing for inspections

Pittsburgh, Pennsylvania

Maintained and ensured integrity of several studies on topics ranging from type 1 diabetes to late-life depression and anxiety Processed both structural and functional data acquired from MRI scans using a wide range of imaging processing software Wrote, ran and provided technical support for software scripts that automate imaging and neuropsychological data processing Taught functional tasks to research subjects and conducted them in the MRI control room Co-authored two research articles for peer-reviewed journals including Journal of Geriatric Psychiatry and Neurology, and contributed to multiple abstracts and presentations Completed descriptive data analysis and statistical modeling, and prepared materials for presentations, manuscripts, and grants proposals

Performed in vivo and in vitro data collection to characterize the cellular and molecular role of Pituitary adenylate cyclase activating protein (PACAP). Dr. May's team also coordinated efforts with behavioral neuroscientists in the Psychology department to explore the role of PACAP in stress and anxiety Performed radio-immunoassays and ELISA to quantify pituitary adenylate cyclase activating protein (PACAP) concentrations in specific brain regions Assisted with primary and stable cell transfections to characterize PAC1 receptor signaling and pharmacokinetics Facilitated PCR and western blot experiments to identify neuronal substrates activated downstream of PAC1 receptor stimulations Studied cryostat histology techniques and applications Maintained basic laboratory upkeep

Aided in behavioral neuroscience experiments exploring the role of PACAP in stress and anxiety Collected real-time in vivo data in rats  Assisted with tissue perfusions and various surgical procedures such as PACAP peptide infusions Completed descriptive data analysis and statistical modeling Maintained general laboratory solutions stock

Master Of Science - Ms, Neuroscience

Biochemistry, Neuroscience

B.S. Biochemistry