David Hansen Email & Phone Number

Måløv

Master the Technology of mRNA Display in Novo Nordisk using the display platform to select and Discover new binders or peptides for Human disease.Expert in qPCR, , DNA purification, RNA purification, Transcription, Reverse transcription and in vitro translation.Expert in instruments such as miSEQ NGS, Kingfisher robots, qPCR machines and also in SPPS on Intavis robot for making 96 Well peptides synthetic.High-Through-Put using the IVVT Technology for expression peptides found in mRNA display.Acylation of fatty acid to IVTT express peptides and further testing with BLI-assay.Maintain the laboratory and been responsible for all lab-equipment and consumes used in the laboratory. Helping PhD’s, Master-students in the laboratory.Supervisor for lab-tech-students.

Denmark

96 well cloning and transformation in Prokaryotes and Eukaryotes , Robot and machine learning, primer and cloning design for automation. Cross functional learning cycles between WT, PE and Production Strain.

Copenhagen Area, Denmark

Microbiology - Gene Technology - DNA and Cloning in B.subtilis and B.licheniformis - PCR - CRISPR - Gene editing - purification - Production strains - Expression of different kind of enzymes. Automation (High througput, Colony picking, small fermentations) DNA sequence (NGS, Sanger).

Copenhagen Area, Denmark

Microbiology - Gene expression in Yeast - Hightroughput screening - Cloning of new variants GLP-1's and Insulin's - Southern blot and Western Blot - LCMS analyze and sample preparation plus DATA - Q-pix screening - Small scale fermentation.

Copenhagen Area, Denmark

Student for one year then permenent employed - DNA and Cloning work in B.subtilis and B.licheniformis - SDS-PAGE - Small scale fermentation - DNA Array - Production strains work - Gene expression systems. From 2010 new department BRASKEM - Metabolic engineering - expression systems in E.coli - Working with Lactos Bacillus - Synthetic genes.