David Mcgarry Email & Phone Number

Cambridge, England, United Kingdom

Toronto, Canada Area

• Previous work undertaken at Cancer Research UK facilitated the opportunity to expand my project into drug development and transgenic mouse studies. As part of this project I produced recombinant protein using bacteria.
• SDS-PAGE, Western blotting, co-immunoprecipitation, enzyme kinetic assays, biotin ligase proximity ligation assays (bio-ID), cell proliferation, cell migration assays, cell invasion assays.

• My research project examined how reactive oxygen species affects cancer invasion through their influence on the actin cytoskeleton. Using mass spectrometry, NGS, CRISPR, and fluorescence microscopy, I was able to show.
• mass spectrometry, proteomics, isobaric labelling of proteins for mass spectrometry, cell culture, lentiviral transduction, siRNA and shRNA, cytotoxic screening, flow cytometry, mitochondrial respiration assays.

I used mass spectrometry to examine how protein bound glutathione can affect protein activity and influence cell toxicity.

My project was to examine posttranslational modifications in recruiting lysine-specific demethylase 1 (LSD1) to chromatin and other protein complexes in cancer. I established knock-in transgenic mouse models of LSD1 and developed biochemical assays to assess protein complex formation in vitro as well as in mammalian culture.

Doctor Of Philosophy (Ph.D.), Toxicology

Thesis title; "Catalytic and non-catalytic mechanisms involved in Glutathione S-transferase Pi mediated cytoprotection"

Bachelor Of Science (Bsc), Pharmacology