David Krolewski

David Krolewski Email and Phone Number

Lead Research Specialist @ University of Michigan Medical School
Ann Arbor, MI, US
David Krolewski's Location
Ann Arbor, Michigan, United States, United States
David Krolewski's Contact Details

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About David Krolewski

I am currently a systems neuroscientist at the University of Michigan Medical School with expertise in neuroanatomical function related to physiological stress and psychiatric disorders. Through this knowledge, I’ve been able to analyze proteins and mRNA molecules that affect brain function and dictate human behaviors. My inspiration for this line of work is directly related to receiving my B.S. from Grand Valley State University in Health Science where I became interested in understanding multiple aspects of human anatomy and physiology. Following this, I worked as a research technician at the Ann Arbor Veteran’s hospital where I attended autopsies, directed by the County Medical Examiner, and subsequently looked at neurotransmitter transporter proteins in postmortem brain of people who abused cocaine. Following this experience, I went on to receive my Ph.D. from Wayne State University Medical School, within the Department of Anatomy and Cell Biology, where my research focused on Parkinson’s disease models and the neurocircuitry that regulates motor activity. Somewhat switching gears, I was fortunate to do my postdoctoral fellowship at the University of Michigan Medical School and investigate neuronal gene expression in postmortem subjects previously diagnosed with psychiatric disorders including major depressive disorder, bipolar disorder, and schizophrenia. I obtained the status of a trusted human brain dissection member that is able to identify and isolate key postmortem brain regions relevant to human neuropathology. This work involves a large collaborative effort by the Pritzker Research Consortium (University of Michigan, University of California Irvine, Stanford University, Cornell University, and the Hudson Alpha Institute) which is dedicated to understanding the underpinnings of mental health. Advancing to a research faculty member at the University of Michigan Medical School, I co-instructed an undergraduate human neuroanatomy course and continue examining neurogenetic changes in psychiatric illness by incorporating advanced microscope-driven methods to visualize and quantify molecular changes using advanced histological techniques. Collectively, these experiences have given me the opportunity to regularly present and discuss experimental data at the annual international Society for Neuroscience conferences for over 20 years and mentor numerous undergraduate students seeking further postgraduate education.

David Krolewski's Current Company Details
University of Michigan Medical School

University Of Michigan Medical School

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Lead Research Specialist
Ann Arbor, MI, US
David Krolewski Work Experience Details
  • University Of Michigan Medical School
    Lead Research Specialist
    University Of Michigan Medical School
    Ann Arbor, Mi, Us
  • University Of Michigan Medical School
    Assistant Research Scientist
    University Of Michigan Medical School Aug 2018 - Present
    Ann Arbor, Mi
    Examinined the regulation of interneuron-localized molecules in the the cerebral cortex of postmortem subjects diagnosed with schizophrenia, bipolar disorder, major depression, and related animal models. In the of pursuit of understanding these conditions, I employ quantitative 3-dimensional neuroimaging techniques, fluorescent and radioactive-based in situ hybridization, and addional immunohistochemistry methods. I also continued to enjoy co-instructing Neuroscience 470, Human Neuroanatomy… Show more Examinined the regulation of interneuron-localized molecules in the the cerebral cortex of postmortem subjects diagnosed with schizophrenia, bipolar disorder, major depression, and related animal models. In the of pursuit of understanding these conditions, I employ quantitative 3-dimensional neuroimaging techniques, fluorescent and radioactive-based in situ hybridization, and addional immunohistochemistry methods. I also continued to enjoy co-instructing Neuroscience 470, Human Neuroanatomy (4 credit lecture/lab) at the University of Michigan and advising lab personnel and research assistants. Show less
  • University Of Michigan Medical School
    Research Investigator
    University Of Michigan Medical School Jan 2014 - Aug 2018
    Optimized brain transparency techniques including CLARITY, iDISCO, and CUBIC for neuroimaging specific circuity and neurovasculature. As part of the Pritzker Research Consortium, I've helped to regularly dissect multiple brain regions used for major mental health studies across several institutions. In addition, while also using animal models, I regularly mentored students and research assistants regarding scientific methods and time management . Also, I have helped co-instruct and develop… Show more Optimized brain transparency techniques including CLARITY, iDISCO, and CUBIC for neuroimaging specific circuity and neurovasculature. As part of the Pritzker Research Consortium, I've helped to regularly dissect multiple brain regions used for major mental health studies across several institutions. In addition, while also using animal models, I regularly mentored students and research assistants regarding scientific methods and time management . Also, I have helped co-instruct and develop Neuroscience 470, Human Neuroanatomy (4 credit lecture/lab) at the University of Michigan. I assisted in syllabus and exam design, delivered lecture material, constructed clinical problem sets, and provided hands-on laboratory instruction implementing human brain samples to undergraduate students. Students were assisted in further course material comprehension through regular office hours. I also enjoyed being an instructor for Molecular/Cell and Developmental Biology 300, Independent Research (4 credit lab), where I facilitated the the applications of molecular and histological methods and their relevance to neuroscience/neuroanatomical-based research projects. The goal was to achieve competence in laboratory techniques as well as principles of scientific writing/summarization of subsequent data destined for potential publication. Show less
  • University Of Michigan Medical School
    Research Fellow
    University Of Michigan Medical School Jan 2006 - Dec 2013
    Using in situ hybridization and histological techniques, I investigated the genetic differences in the human brain regions associated with the pathology of depression, bipolar disorder, and schizophrenia. More specifically, my focus was directed toward analysis of the human hypothalamus nuclei that regulate stress responses, feeding behaviors, and sleeping patterns.
  • Ann Arbor Veterans Hospital
    Research Assistant
    Ann Arbor Veterans Hospital 1999 - 2001
    Attended autopsies of those who passed away from cocaine abuse to obtain relevant brain tissue samples. I performed both western blot-driven protein analysis of the basal ganglia as well as histological examination of thinly sectioned brain slices. The results of these studies showed a reduction of VMAT2 protein which indicates cell death in cocaine abuse.

David Krolewski Skills

In Vivo Histology Animal Models Elisa Immunohistochemistry Genetics Confocal Microscopy Pathology Molecular Imaging Science Neuroscience Cell Biology Microscopy Fluorescence Microscopy

David Krolewski Education Details

Frequently Asked Questions about David Krolewski

What company does David Krolewski work for?

David Krolewski works for University Of Michigan Medical School

What is David Krolewski's role at the current company?

David Krolewski's current role is Lead Research Specialist.

What is David Krolewski's email address?

David Krolewski's email address is dk****@****ich.edu

What is David Krolewski's direct phone number?

David Krolewski's direct phone number is (734)-764*****

What schools did David Krolewski attend?

David Krolewski attended Wayne State University School Of Medicine, Grand Valley State University.

What skills is David Krolewski known for?

David Krolewski has skills like In Vivo, Histology, Animal Models, Elisa, Immunohistochemistry, Genetics, Confocal Microscopy, Pathology, Molecular Imaging, Science, Neuroscience, Cell Biology.

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