David Splan Email & Phone Number

Ann Arbor, Mi, Us

Ann Arbor, Mi, Us

Ann Arbor, Michigan, United States

Ann Arbor, Michigan

-Lead team of scientists in the transfer of customer bioprocesses, including protein, viral vaccine and stem cell production, to large scale in an expeditious and efficient manner, leading to an improved, lower-cost process. -Provide targeted optimization and development of scale-up methods using various bioreactor platforms, including the Allegro STR Bioreactor, iCELLis, Xpansion, PadReactor and XRS technologies, with demonstrated consistency as well as comprehensive protocols.-Proven ability to build a strong rapport with customers based on a foundation of respect and trust, leading to relationships that extend beyond the work done within the Process Development Contract.-Work with Project Managers to meet pre-determined scientific and financial milestones, collating and reporting relevant information to both the client and executive management on a monthly basis.-Demonstrated ability to develop a strong team, both inter- and intra-personally, enhancing and broadening skill levels, while achieving company objectives.

Ann Arbor, Michigan

Bioreactor Process Development -Install, configure and adapt multiple bioreactor technologies, including the Allegro STR Bioreactor, PadReactor, New Brunswick Celligen 310, CerCell and Millipore Cell Ready for use with microcarrier cultures for the purposes of large-scale expansion of various cell types, including hMSC, Vero, MRC-5 and HEK 293.-Served as scientific lead on effort toward the development and commercialization of an ancillary bioreactor system component designed to harvest and separate cells from large scale microcarrier cultures in a sterile and fully-closed fashion. -Developed device utilized for the large-scale concentration and simultaneous removal of unwanted products including dissociation enzymes from large volumes (25 L+) of cell suspensions harvested from bioreactors. This would allow for the direct transfer of cells from bioreactor-to-bioreactor in a fully-closed system, dramatically reducing the time, labor and cost associated with this critical step in the large-scale expansion of cells.-Created novel method designed for the measurement of particulate matter within cell suspensions, fully complying with the United States Pharmacopeia (USP) Chapter 788 Guidelines for particulate matter in injections, an absolute requirement for cell therapy applications.Novel Microcarrier Development Program-Interact with senior management to develop strategies necessary to achieve company objectives. -Lead team of scientists on the design and implementation of experiments aimed at development of novel microcarrier surface chemistries. -Developed a portfolio of several prototype microcarriers, each with differing attributes based around charge and/or attachment factor(s). -Fully-validated manufacturing processes and release assays necessary for the large-scale production of mutltiple microcarrier products. -Co-inventor on multiple patent applications for cell culture-related technologies.

Ann Arbor, Michigan

Develop and implement experiments designed to optimize growth of various cell types on microcarriers utilizing multiple bioreactor technologies.-Optimize culture conditions required for growth, harvest and serial passage of several cell types on microcarriers, allowing for expansion from small to large scale. -Discovered molecules required for vigorous growth of cells on one of SoloHill’s leading microcarrier products. -Developed method for detection of surface coating on select microcarrier products, providing an additional release assay toward manufacture of those products.

Ann Arbor, Michigan

-Efficiently managed a cancer research laboratory developing T cell receptor (TCR) based vaccines for the treatment of T cell malignancies. -Designed TCR vaccines that effectively stimulated tumor protection in mice. -Required technical expertise in cell culture, generation of bone marrow-derived dendritic cells, stable and transient transfection procedures and relevant reporter assays, basic cloning techniques, plasmid construction and preparation, PCR optimization, protein purification, quantification, FACS, ELISA, SDS PAGE and western blot analysis. Extensive animal handling experience.