Wei Hsin(David) Cheng Email & Phone Number

My name is David Cheng, a student in the UAB Biotechnology Program. I graduate in Dec 2017 with an MS degree in biotechnology. During the education in UAB, I led a team in Cancer Drug Discovery focusing on the effect of RANK: Traf-2 interaction in breast cancer metastasis, and also had an internship with protein expression and purification in the end of the semester. I am in the process of finding a research position as part of my optional practical training, in the meantime, I am doing a temp specialist in a research to find out if early life stress is associated with depression and obesity in Department of Psychiatry.RESEARCH SKILLS• Bacterial Culture• Homology based Cloning• Plasmid Isolation• Transfection/ Gene Regulation• Drug Design• Mass Spec/ NMR• HPLC/TLC• Toxicology• Protein Purification• Preclinical StudiesPROFESSIONAL EXPERIENCE Cancer Drug Discovery Team Leader • Led a team that studied the effect of RANK: Traf-2 interaction in breast cancer metastasis • Generated deletion mutants in the cytosolic domain of RANK• Cloned deletion mutants into the mammalian expression vector, pcDNA3.1-His TOPO• Expressed deletion mutants in HEK-293 cells• Assessed RANK:Traf-2 interaction using immunofluorescence and co-immunoprecipitation• Used established protocols for data documentation and analysis• Presented findings in a seminar Original Biomedicals Co., Ltd

Listed skills include Laboratory Skills, Animal Models, Organic Chemistry, Molecular Biology, and 8 others.