Pediatric Leukemia - Non-coding sequence variation - Chromatin Biology

Epigenetics - Genome Editing - Single Cell RNA-seq - Hematopoiesis - Stem cell biologySeruggia D, Oti M, Tripathi P, Canver MC, LeBlanc L, Di Giammartino DC, Bullen MJ, Nefzger CM,Sun YBY, Farouni R, Polo JM, Pinello L, Apostolou E, Kim J, Orkin SH, Das PP. TAF5L and TAF6LMaintain Self-Renewal of Embryonic Stem Cells via the MYC Regulatory Network. Mol Cell. 2019 Jun 20;74(6):1148-1163.e7Sher F*, Hossain M*, Seruggia D*, Schoonenberg VAC, Yao Q, Cifani P, Dassama… Show more Epigenetics - Genome Editing - Single Cell RNA-seq - Hematopoiesis - Stem cell biologySeruggia D, Oti M, Tripathi P, Canver MC, LeBlanc L, Di Giammartino DC, Bullen MJ, Nefzger CM,Sun YBY, Farouni R, Polo JM, Pinello L, Apostolou E, Kim J, Orkin SH, Das PP. TAF5L and TAF6LMaintain Self-Renewal of Embryonic Stem Cells via the MYC Regulatory Network. Mol Cell. 2019 Jun 20;74(6):1148-1163.e7Sher F*, Hossain M*, Seruggia D*, Schoonenberg VAC, Yao Q, Cifani P, Dassama LMK, Cole MA,Ren C, Vinjamur DS, Macias-Trevino C, Luk K, McGuckin C, Schupp PG, Canver MC, Kurita R,Nakamura Y, Fujiwara Y, Wolfe SA, Pinello L, Maeda T, Kentsis A, Orkin SH, Bauer DE. Rationaltargeting of a NuRD subcomplex guided by comprehensive in situ mutagenesis. Nat Genet. 2019Jul;51(7):1149-1159 (* equal contribution). Di Pilato M, Kim EY, Cadilha BL, Prüßmann JN, Nasrallah MN, Seruggia D, Usmani SM, Misale S,Zappulli V, Carrizosa E, Mani V, Ligorio M, Warner RD, Medoff BD, Marangoni F, Villani AC, MempelTR. Targeting the CBM complex causes Treg cells to prime tumours for immune checkpoint therapy.Nature. 2019 Jun;570(7759):112-116Debruyne DN, Dries R, Sengupta S, Seruggia D, Day D, Gao Y, Sharma B, Huang H, Moreau L,McLane M, Marco E, Chen T, Gray NS, Wong K, Orkin SH, Yuan GC, Young RA, George RE. TheCTCF paralog, BORIS, promotes novel chromatin-based regulatory interactions in treatment-resistant cancer cells. Nature 2019 Aug;572(7771):676-680.LeBlanc L, Lee BK, Yu AC, Kim M, Kambhampati AV, Dupont SM, Seruggia D, Ryu BU, Orkin SH,Kim J. Yap1 safeguards mouse embryonic stem cells from excessive apoptosis during differentiation. Elife. 2018 Dec 18;7 Show less

3D chromatin organization - Epigenetics - CRISPR-Cas9 genome editingFunctional validation of mouse tyrosinase non-coding regulatory DNA elements by CRISPR–Cas9-mediated mutagenesisSeruggia D, Fernández A, Cantero M, Pelczar P, and Montoliu LNucleic Acids Research (2015)MIR retrotransposon sequences provide insulators to the human genome.Wang J, Vicente-García C, Seruggia D, Moltó E, Fernandez-Miñán A, Neto A, Lee E, Gómez-Skarmeta JL, Montoliu L, Lunyak VV… Show more 3D chromatin organization - Epigenetics - CRISPR-Cas9 genome editingFunctional validation of mouse tyrosinase non-coding regulatory DNA elements by CRISPR–Cas9-mediated mutagenesisSeruggia D, Fernández A, Cantero M, Pelczar P, and Montoliu LNucleic Acids Research (2015)MIR retrotransposon sequences provide insulators to the human genome.Wang J, Vicente-García C, Seruggia D, Moltó E, Fernandez-Miñán A, Neto A, Lee E, Gómez-Skarmeta JL, Montoliu L, Lunyak VV, Jordan IK.PNAS (2015) Show less

Chromatin & transcription. Genome engineering with TALENs and CRISPR/Cas9The new CRISPR–Cas system: RNA-guided genome engineering to efficiently produce any desired genetic alteration in animals.Seruggia D and Montoliu L Transgenic Research (2014) Mouse genome editing using CRISPR/Cas system.Harms D, Quadros R, Seruggia D, Ohtsuka M, Takahashi G, Montoliu L and Gurumurthy CB Current Protocols (2014)The PEG13-DMR and brain-specific enhancers dictate… Show more Chromatin & transcription. Genome engineering with TALENs and CRISPR/Cas9The new CRISPR–Cas system: RNA-guided genome engineering to efficiently produce any desired genetic alteration in animals.Seruggia D and Montoliu L Transgenic Research (2014) Mouse genome editing using CRISPR/Cas system.Harms D, Quadros R, Seruggia D, Ohtsuka M, Takahashi G, Montoliu L and Gurumurthy CB Current Protocols (2014)The PEG13-DMR and brain-specific enhancers dictate imprinted expression within the 8q24 intellectual disability risk locus. Court F, Camprubi C, Vicente-Garcia C, Guillaumet-Adkins A, Sparago A, Seruggia D, Sandoval J, Esteller M, Martin-Trujillo A, Riccio A, Montoliu L and Monk D Epigenetics and Chromatin (2014) Show less

Generation of transgenic mice, development of new recombinase systems.Binary recombinase systems for high-resolution conditional mutagenesis.Hermann M, Stillhard P, Wildner H, Seruggia D, Kapp V, Sánchez-Iranzo H, Mercader N, Montoliu L, Zeilhofer HU and Pelczar P.Nucleic Acids Research (2014)

Single Molecule Techniques, Chromatin in vitro reconstitution.Magnetic tweezers measurements of the nanomechanical properties of DNA in the presence of drugs.Salerno D, Brogioli D, Cassina V, Turchi D, Beretta GL, Seruggia D, Ziano R, Zunino F, Mantegazza F.Nucleic Acids Research (2010)Atomic force microscopy study of DNA conformation in the presence of drugs.Cassina V, Seruggia D, Beretta GL, Salerno D, Brogioli D, Manzini S, Zunino F, Mantegazza F.Eur… Show more Single Molecule Techniques, Chromatin in vitro reconstitution.Magnetic tweezers measurements of the nanomechanical properties of DNA in the presence of drugs.Salerno D, Brogioli D, Cassina V, Turchi D, Beretta GL, Seruggia D, Ziano R, Zunino F, Mantegazza F.Nucleic Acids Research (2010)Atomic force microscopy study of DNA conformation in the presence of drugs.Cassina V, Seruggia D, Beretta GL, Salerno D, Brogioli D, Manzini S, Zunino F, Mantegazza F.Eur Biophysics Journal (2011) Show less

Cell Biology, gene therapy, non-viral episomal vectors, inducible expression.Controlled removal of a nonviral episomal vector from transfected cells.Rupprecht S, Hagedorn C, Seruggia D, Magnusson T, Wagner E, Ogris M, Lipps HJ.Gene (2010)

Genotyping of transgenic animals, expression vectors design.