David Pescetti Email & Phone Number

South San Francisco, California, US

South San Francisco, California, US

Forchheim, DE

• Programmer and tester of new workflows for updates to VERSANT kPCR Sample prep system (Hamilton Starlet)
• V&V of MiPLX 2.0 software for QuantStudio5
• Integrated newly acquired company into SIEMENS Healthineers Molecular Diagnostics platforms
• Design, verify, validate new and revamped Liquid Classes/TADMs

Brisbane, California, US

• Lead programmer on Hamilton Starlet LHIs for newly launched clinical sequencing laboratory
• Driver of continuous process improvements of methods and techniques within multiple automation workflows
• Create and train end users on SOPs for FDA, CLIA, and CAP cleared clinical laboratories
• Internal Auditor for compliance with FDA and internal quality standards
• Vendor and contract management

Brisbane, California, US

• Program, troubleshoot, repair, QC, and maintain Automation instruments for a FDA, CLIA, CAP certified Clinical lab, CE marked GMP Manufacturing facility, and R&D lab.
• Work with Beckman LHIs, ABI 7900HT RT-qPCR, BML XL20, SpectraMax platforms
• Write and train users on SOPs for an FDA, CLIA, CAP cleared clinical laboratory
• Design, implement, and maintain a camera monitoring system for LHIs
• Administrator, and coordinator for cold storage device and monitoring systems

San Luis Obispo, CA, US

• Design, fabrication, and validation of microfluidic, chemical separation device for integration into a point of care system. Development, optimization, and implementation of novel experimentation protocol.
• Device validation and analysis via COMSOL and SIMUL simulations
• Model design with CAD software including Solid Works and AutoCAD
• Fabrication of MEMS device in class 1000 clean room – soft lithography, rapid prototyping
• Surface chemistry and coating validations through goniometry and EOF calculations

• Worked within a multidisciplinary group tasked with validating target genomic integration. Implemented automation of genomic verification assays.
• Automation of genomic DNA extraction using the Tecan Genesis Freedom 200
• Critical evaluation and design of multiple automation protocols
• Tissue screening for validation of target gene integration
• Facilitated extensive, multi-location international trials for FDA submission

US

• Genotyping HLA alleles for the Type 1 Diabetics Global Consortium (T1DGC). Assessed allelic contribution to the risk of developing Type 1 Diabetes.
• Genotyping Class II HLA-DQ locus via reverse linear assay, utilizing Sequence Specific Oligonucleotide Probing (SSOP)
• Evaluation using Stripscan and Sequence Compilation and Rearrangement Evaluation (SCORE)
• Demonstrated of findings in a symposium presentation

Master'S Of Science, Biomedical Engineering

Bachelor Of Science, Biomedical Engineering

Education record