Laboratory Manager at Santa Cruz Biotechnology with worldwide distribution of over 23,000 specific monoclonal antibodies10+ years management 20+ years Biotechnology backgroundCRISPR knockout and activation (CRISPR / Cas9, HDR, Double Nickase, Activation, Lentiviral activation particles) studies and product developmentHigh utilization of: Excel, Word, PowerPoint,Access, Outlook, Mail, Photoshop, FileMaker Pro Advanced, Adobe Illustrator, Windows 10, IOS 14, GEN5, AxioVision, MAC, PCCOVID-19 SOP creation / implementation / oversight both domestically and overseesImport / export laboratory generated materials for research / production at oversees facilityStartup of Immunohistochemistry and Biosafety Level II Cell Culture laboratory overseesLaboratory equipment procurement and validationResearch & DevelopmentQuantitative/Qualitative AnalysisBiotech /Pharmaceutical ServicesProject Coordination & ManagementTraining & DevelopmentQuality ControlProduct /Protocol DevelopmentMaterials OversightCulture of mammalian cell lines and hybridomas Antibody purification from serum and cultured hybridoma cultures Enzyme-linked immunosorbent assays (ELISA) Hazardous waste and medical waste handling and disposal In vitro transfection of plasmid siRNA, shRNA, cDNA, and CRISPR Transformation of E. coli bacteria Antibody development, conjugation, and staining Flow Cytometry Bacterial (mycoplasma) culture infection detection and treatment / eradicationCarbon Dioxide application for culturing incubatorsWestern blot for protein and antibody analysis RT-PCR (Real Time-Polymerase Chain Reaction) Biosafety Level 1 and 2 materials handling Tissue sectioning using microtome / cryotome Cryogenic storage of cell banks Aseptic techniquesHPLC (High Performance Liquid Chromatography)Chemical induction of cell linesColumn chromatographyUV/Vis spectrophotometryMicroscopyFluorescent microscopyUltraviolet SpectroscopyMouse inoculation, organ harvestPolyacrylamide gel electrophoresis Cell line transfection / transductionSOP creation / implementation / oversight Protein isolation and characterizationDatabase creation Employee hiring / training / managementEstablished and implemented internal biosafety proceduressiRNA, shRNA, and cDNA transfection studiesMammalian cell line, hybridoma antibody, and human tissue extract developmentImmunofluorescent validation of monoclonal antibodies and publication of imagesVSV-G pseudoviral particles (shRNA / CRISP transformed) production and validationBoard of DirectorsContract pricing negotiations