Diana Carolina Chiluiza, Phd Email & Phone Number

Boston, Ma, Us

Hicksville, Ny, Us

Immune Cell Products: BioIVT provides the cell isolation expertise needed to deliver high-quality PBMCs and other immune cell subsets. With our rigorous QC testing, we ensure reproducible results and eliminate upstream variability. We opened a new cell isolation facility to serve our customers in the Greater Boston Area, United States and Europe.Business Process Development. I perform analytical analysis of immune cells production and quality control of isolated all immune cell type products. I established and manage the immune cell isolation facility. SOPs. Protocol reviews. Data capture. Customer support. Collaboration with Business Development team.Data strategies: I also leverage immune cell products data to help drive business decisions, optimize processes, and create strategic advantages. This includes the development of data strategies, analysis, implementation of data technologies, complete product specifications, and performance measurements to drive continuous improvements.

Boston, Ma, Us

Master Program in Analytics, College of Professional Studies.I teach the following courses: (a) Introduction to Analytics, (b) Introduction to Statistics and Probably Theory, and (c) Communication and Visualization for Data Analytics.I teach statistical concepts and practical applications in data capture, advanced analytics, hypothesis testing, and data-based decision making.https://cps.northeastern.edu/faculty/dee-chiluiza-reyes/• Two years in a row nominated to the University's Excellence in Teaching Award.

Boston, Ma, Us

The Association of Ecuadorians in New England (AENI) is a registered 501(C)(3) non-profit organization with the mission of delivering basic services to underprivileged children in Ecuador. AENI also serves as a forum for Ecuadorians based in New England in which they can unite and generate ideas for promoting the well-being of underprivileged Ecuadorian children.AENI also serves as a forum for Ecuadorians based in New England, in which they can unite and generate ideas for promoting the well-being of underprivileged Ecuadorian children. We also provide a common ground for volunteers and benefactors alike to foster the awareness of needs which are direct consequences of extreme poverty in Ecuador. Through its events and membership benefits, AENI fundraises for charitable initiatives in health, education, and other areas of importance.More information at: https://aeni.org/impact.php

Based of ExQor multi-patented bio-nanotechnology to develop novel treatments for Alzheimer's disease and other disorders of the CNS.Lab design/establishment: Responsible for the establishment of the molecular biology/biochemistry laboratory and cell culture facility. Production: Established the technologies for the production, purification, and validation of recombinant proteins. Genetic engineering: Designed and constructed expression vectors for bio nanoparticle production.Cell culture. Multi-layer culture of WT HEK293 for up-scale production of nanoparticles. cGMP/SOPs: Lead the development of processes and SOPs for transfer to Good Manufacturing Practice (GMP) Core Facility.GMP stable cell line construction: Master cell lines developed for up-scaled protein production - bacterial (DH5α) and mammalian (HEK293) cell lines -.Management: Trained technical personnel.

Belmont, Ma, Us

Brain Imaging Nanotechnology Group, McLean Research Programs. Visitor Scientist.

Boston, Ma, Us

Affiliations through: Nephrology Department, Beth Israel Deaconess Medical CenterImmunology/Dermatology Department, Brigham and Women's HospitalBrain Imaging Nanotechnology Group, McLean HospitalHarvard Institutes of MedicineBoston, MA 02115

Us

Ec

Scientist and Life Sciences Consultant; appointed by the Ecuadorian Government to foster advanced medical research nationwide through the Executive branch of Research, Innovation and Higher Education. I directly supported work at the National Institute of Research in Public Health, the School of Medicine of University of Guayaquil, Project Prometheus (Government initiative to bring highly trained scientist to the country), and SENESCYT (National Secretary of Education, Science, and Technology). To foster new frontiers in research, I brought and established concepts of Core Facilities and multi-purpose molecular labs (which was surprisingly initially rejected by many groups but finally accepted), organized multi-institutional research groups, goal-oriented research teams, and organized courses in molecular techniques, scientific methodology, seminars, and scientific journal clubs. I also worked with already established research teams and provide support in advanced technologies. I also established the methodologies for the detection and analysis of mRNA molecular markers in diabetic patients using peripheral blood mononuclear cells.

Boston, Ma, Us

1. Cell culture-based genome-wide siRNA High Throughput Screening assay for the discovery of novel regulators of mTORC1 in response to high glucose concentrations. In collaboration with Harvard Medical School ICCB-Longwood Screening Facility. • Analysis of PI3K/AKT/mTOR intracellular signaling pathway role in diabetic nephropathy for the early-stage discovery of diabetic nephropathy biomarkers and potential drug candidates.• Design and optimization of innovative screening concepts that efficiently developed a cell culture model of diabetic nephropathy using human podocytes. • Optimized High-Throughput Quantitative Immunofluorescence assays.• Optimized the use of high-resolution plate readers, robotics, automated liquid handlers.2. Cell culture-based analysis of molecular mechanisms altered by gain-of-function TRPC6 mutations and role in the development of focal and segmental glomerulosclerosis• Analysis of Ras/Raf/MAPK/ERK signaling pathway role in alterations of podocyte activity and loss of kidney function.

New Orleans, Louisiana, Us

Cell culture-based oncology model for the analysis of molecular mechanisms of mammary tumor development induced by gain of function mutations of eIF3e» Development a cell culture model that mimics expression of Mouse Mammary Tumor Virus (MMTV) insertion from a single allele. » Design and optimization of in vitro and biochemical assays to study mechanisms regulated by truncated eIF3e at the translation initiation level. » High-resolution isolation of light versus heavy polysomal complexes to analyze mRNA translation profiles that led to the discovery of mechanisms of malignant transformation based on a shift between cap-dependent to cap-independent recruitment of endogenous mRNAs to translation machinery. In vivo analysis of protein-protein interactions in translation initiation complexes by various isoforms of translation initiation factor 4E (eIF4E) in Caenorhabditis elegans » Overexpression of eIF4E is observed in several types of cancer. » C. elegans used as an oncology model to study mechanism of malignant transformation by eIF4E overexpression. » Optimization of up-scale protein production, affinity chromatography, high-performance liquid chromatography, and MALDI-TOF mass spectrometry assays that lead to the identification of protein interactions data that supported PI Grant proposal.

http://gcrl.usm.edu/» I worked in the laboratory of Dr. Williams E. Hawkins, PhD, in the marine aquaculture group conducting research related to the restocking of natural populations of red snapper in the Gulf of Mexico.» I made a highly detailed description of the morphological development of the digestive system of red snapper from hatch to day 30.» Red snapper is an important commercial fish species for several Southern U.S. States. Extensive fisheries affected the natural population while re-stocking efforts were affected by high mortalities during larval » I proposed and optimized a high‑resolution plastic‑embedding light microscopy technique for complete serial sagital and transversal sectioning of fish larvae.» I also optimized the use of digital photography to create 3D models of internal organs of fish larvae.» I optimized the use of scanning electron microscopy to study the external characteristics of fish larvae and demonstrated that early high mortality rates occurred simultaneously with rapid morphological changes while endogenous food supplies have been depleted and larvae were not ready to receive their first exogenous feeding.» Thesis published in World Catalog Libraries (Worldcat.org), OCLC Number: 56079222

Development of an innovative program in Ecuador to provide educational and animal assisted therapies to disadvantaged children, including orphans in state facilities, children living in the streets, children accompanying their mothers at the Women Correctional Facility, and children working to support their families. » Program supported by UNICEF (France) and the Government of Ecuador.» Led the development of the program.» Strategically formed interest and goal-based teams for the development of protocols.» Coordinated inter-institutional relationships and applied novel concepts in the country. » Developed, established, and managed educational center, educational farm, and field-environmental trips, overseeing and approving activities. » Proposed and led the inclusion of ancestral indigenous communities.» Coordinated budget management, staff and volunteers training and motivation, and preparation of reports and presentations to funding agencies.

Doctor Of Philosophy (Ph.D.), Biochemistry And Molecular Biology

Master Of Science (M.S.), Coastal Sciences

Bachelor Of Science (B.S.), Biology