Drug Development process, assisting in Human Clinical trials, Re-purposing FDA-approved drugs.Developing In vitro studies for screening FDA-approved drugs(Immunomodulators) for Stroke.Link- https://med.emory.edu/departments/medicine/divisions/infectious-diseases/studies-programs/mwccs/interested-participants/macswihs-ccs-substudies/gut-res/meet-the-team/index.html

• Understanding the physiology of cystic fibrosis by developing the patient-derived organoid model using Matrigel, fibroblast, and EpCAM+ve cell.• Understanding the gene signature of disease by preparing samples for 10X genomics single-cell RNA seq and analyzing through R-programming.• Optimizing the protocol for single nuclei ATAC-seq to understand the molecular patterns of sequences in open chromatin in cystic fibrotic patients.• Understanding the molecular targets in Amyotrophic Lateral Sclerosis by differentiating iPSC into i-motor neurons(i-MN) of ALS patients.• Understanding ALS -specific gene expression patterns via RT-qPCR from samples derived at different time points while differentiating in iMNs.• Screened H&E histological samples of ALS patients to understand the size difference between ChAT-positive motor neurons in ALS patients vs the Control through FlowJo software. Experience in oral presentations of scientific data and concepts with strong proficiency in the biotechnology field, as demonstrated by successfully delivered cross-departmental seminars.

Project: Transcriptome and interactome analyses identify the TP53 interacting gene RCCD1 as a candidate susceptibility gene at15p26.1 breast and ovarian risk locus• Developed functional-based assay to validate TP53 interacting genes with RCCD1 in mutant cell lines (12 mutant) derived from BCCR and OCCR regions of BRAC1 and BRAC2 genes.• Understanding the TP53 signaling via DNA damaging drugs (Etoposide) and the difference in P53 signaling mutant cell lines by cell proliferation assays (Real-time Glo), RT-qPCR, western blotting, Immunofluorescence staining, and analysis through confocal laser scanning microscope.

Project: Upregulation of canonical Wnt signaling may underlie aggressive disease course in androgen receptor-negative Breast Cancer.• Performed western blotting to understand the Wnt signaling in AR knockout cell lines.• Data mining for the receptor-negative tissue by understanding the clinical data of the TNBC patients at Dekalb Hospital

• Teaching 30 students weekly in biology class• Improved 65% of tutored student's test scores

Project - Inhibition of 12/15 LOX ameliorates cognitive and cholinergic dysfunction in a mouse model of hypobaric hypoxia via. attenuation of oxidative/nitrosative stress.- Help in developing a mouse model for hypobaric hypoxia

• Isolated DNA from leukocytes and performed spectrophotometric measurement of DNA.• Prepared cells for calcium measuring by Spectro fluorophotometer to check the levels of calcium during the desensitization of G-protein coupled receptor during the inflammation.• Measured RNA expression of specific genes by qPCR which was involved in the molecular pathway of inflammation and hands-on experience on Sanger sequencing.

Project: Role of Dermal stem cell in aiding re-pigmentation in Vitiligo patients• Standardized H&E protocol for tissue processing and histology of skin samples.• Cultured dermal stem cells for infusing them in affected areas

• Immunophenotyped and analyzed various blood malignancies of the patient sample via Coulter570.• Cultured and differentiated CD34 hematopoietic stem cells.• Isolated PBMNC from the bone marrow from patient samples.• Detected the functionality of Platelets for platelet Dysfunction.• Cryopreserved human stem cells and bone marrow for follow-up • Data Analysis via SPSS