Deepthi Kiran Email & Phone Number

Bengaluru, Ka, In

Bengaluru, Karnataka, India

Bengaluru, Karnataka, India

Bengaluru, Karnataka, India

My job responsibility includes teaching and training B.Sc and M.Sc (Microbiology and Biotechnology) students in the subjects assigned by Bangalore university. Guiding them in the lab, projects, designing the experiment for research activity and for paper publication.

Bengaluru, Karnataka, India

Research on transboundary and endemic animal diseases in North Eastern region , so as to develop core competence on rapid diagnosis and control of such diseases .Use of molecular diagnostic kits to detect diseases and sampling techniques. A recombinant Erns glycoprotein based ELISA for the detection of anti-CSFV antibodies was done. To this end, Erns gene amplified from a field isolate of Karnataka CSFV/ADMac/4651/16) is cloned into pET 32a vector for transfer into BL21(DE3) pLysS cell for… Show more Research on transboundary and endemic animal diseases in North Eastern region , so as to develop core competence on rapid diagnosis and control of such diseases .Use of molecular diagnostic kits to detect diseases and sampling techniques. A recombinant Erns glycoprotein based ELISA for the detection of anti-CSFV antibodies was done. To this end, Erns gene amplified from a field isolate of Karnataka CSFV/ADMac/4651/16) is cloned into pET 32a vector for transfer into BL21(DE3) pLysS cell for expression. The expressed protein is purified and hyperimmune sera was raised in Guinea pigs and confirmed by Westren blot. Further the protein used to detect the CSFV infection in pigs by ELISA Show less

Cell Culture: Cell culture maintenance (BHK 21, 293 cells), Cell counting, Scaling up of cells, aseptic handling of cell culture facility, media preparation and filtration.Virology:Bulk virus production of Ad5-FMD virus in 293cells and FMD viruses in BHK cell lines, Harvesting of viruses, Haemagglutination assays, Titration of viruses, Serum Neutralization tests, Transfection, BEI inactivation and PEG precipitation for FMDV antigen preparation. Purification of viruses by… Show more Cell Culture: Cell culture maintenance (BHK 21, 293 cells), Cell counting, Scaling up of cells, aseptic handling of cell culture facility, media preparation and filtration.Virology:Bulk virus production of Ad5-FMD virus in 293cells and FMD viruses in BHK cell lines, Harvesting of viruses, Haemagglutination assays, Titration of viruses, Serum Neutralization tests, Transfection, BEI inactivation and PEG precipitation for FMDV antigen preparation. Purification of viruses by CsCl ultracentrifugation & Dialysis.Molecular Biology: Plasmid Isolation, Transformation, Restriction Digestion, Agarose Gel electrophoresis, Gel elution, SDS PAGE, Cloning . Show less

Bangalore

I worked towards collection of milk samples from various organised and unorganised sectors, screening for subclinical mastitis milk, isolation and identification of organisms by staining and by biochemical methods. Molecular characterisation of organisms in to genus and species level by PCR method. In-vitro capsular typing and antibiotic sensitivity test for the screening of methicillin resistant S.aureus and was confirmed by PCR.1. Media preparation, staining, microscopy, sample… Show more I worked towards collection of milk samples from various organised and unorganised sectors, screening for subclinical mastitis milk, isolation and identification of organisms by staining and by biochemical methods. Molecular characterisation of organisms in to genus and species level by PCR method. In-vitro capsular typing and antibiotic sensitivity test for the screening of methicillin resistant S.aureus and was confirmed by PCR.1. Media preparation, staining, microscopy, sample collection and processing for isolation and identification, of various bacteria by biochemical and molecular method like PCR.2.Immunodiffusion test , Immunoelectrophoresis, agglutination and agglutination inhibition tests ElISA. Show less

Bangalore

Osteoporosis is a common problem at old age , the reason being mal absorption of calcium in the body and wearing out of bones and joints.The objective of this project is to formulate the fingermillet based bioactive peptide by using hydrolysed product obtain from the whey,soya and from milk protein i,e casein.From the experiments we have proved that caseinophosphopeptide obtain from the milk protein hydrolysis have the ability to bind the calcium which is present in the calcium rich… Show more Osteoporosis is a common problem at old age , the reason being mal absorption of calcium in the body and wearing out of bones and joints.The objective of this project is to formulate the fingermillet based bioactive peptide by using hydrolysed product obtain from the whey,soya and from milk protein i,e casein.From the experiments we have proved that caseinophosphopeptide obtain from the milk protein hydrolysis have the ability to bind the calcium which is present in the calcium rich fingermillet and will be absorbed in the interstine without further digestion . Further , it will be advantageous to provide easily digestable hydrolysed whey and soya protein which will help in reabsorbing of the bone and help to maintain the growth of body tissue. Analysed the influence of bioactive peptide enriched functional food on the growth and bone calcification on experimental animals. Show less

Bangalore

screening of diseases like Brucellosis, , PPR (Pestities de petites ruminants), Para tuberculosis by ELISA(competitive and sandwich), Immunogical test like Haemaggultination test, Agarose Gel Immunodeficiency.Isolation of mycoplasma by cultural method from the field samples and molecular confirmation by PCR method. Responsible for cultural maintainence. Standardisation of the long term viability of the stored Mycoplasma by freeze drying method. Dedecting the microbial titer by colony… Show more screening of diseases like Brucellosis, , PPR (Pestities de petites ruminants), Para tuberculosis by ELISA(competitive and sandwich), Immunogical test like Haemaggultination test, Agarose Gel Immunodeficiency.Isolation of mycoplasma by cultural method from the field samples and molecular confirmation by PCR method. Responsible for cultural maintainence. Standardisation of the long term viability of the stored Mycoplasma by freeze drying method. Dedecting the microbial titer by colony count. Rising of hyper immune sera in the animal against the same Show less

Master'S Degree, Microbiology, General, 1St

M.Sc DESSERTATION: “Bioprospecting of microbial endophytes and their metabolites” Endophytes are the microorganism residing in living.

Bachelor'S Degree, Botany, Zoology, Chemistry, 1St With Distinction